Judith Margarete Gottwein

2019

Evolutionary Pathways to Persistence of Highly Fit and Resistant Hepatitis C Virus Protease Inhibitor Escape Variants

Jensen, S. B., Fahnøe, U., Pham, L. V., Serre, S. B. N., Tang, Q., Ghanem, L., Pedersen, M. S., Ramirez, S., Humes, D., Pihl, A. F., Filskov, J., Sølund, C. S., Dietz, J., Fourati, S., Pawlotsky, J. M., Sarrazin, C., Weis, N., Schønning, K., Krarup, H. & Bukh, J. & 1 others, Gottwein, J. M., Sept 2019, In: Hepatology. 70, 3, p. 771-787 17 p.

Research output: Contribution to journal › Journal article › Research › peer-review

12 Citations (Scopus)

19 Downloads (Pure)

HCV genotype 1-6 NS3 residue 80 substitutions impact protease inhibitor activity and promote viral escape

Pham, L. V., Jensen, S. B., Fahnøe, U., Pedersen, M. S., Tang, Q., Ghanem, L., Ramirez, S., Humes, D., Serre, S. B. N., Schønning, K., Bukh, J. & Gottwein, J. M., Mar 2019, In: Journal of Hepatology. 70, 3, p. 388-397

Research output: Contribution to journal › Journal article › Research › peer-review

17 Citations (Scopus)

2018

Antiviral Effect of Ribavirin against HCV Associated with Increased Frequency of G-to-A and C-to-U Transitions in Infectious Cell Culture Model

Galli, A., Mens, H., Gottwein, J. M., Gerstoft, J. & Bukh, J., 1 Dec 2018, In: Scientific Reports. 8, 1, 13 p., 4619.

Research output: Contribution to journal › Journal article › Research › peer-review

16 Citations (Scopus)

56 Downloads (Pure)

Efficacy of NS5A Inhibitors Against Hepatitis C Virus Genotypes 1–7 and Escape Variants

Gottwein, J. M., Pham, L. V., Mikkelsen, L. S., Ghanem, L., Ramirez, S., Scheel, T. K. H., Carlsen, T. H. R. & Bukh, J., Apr 2018, In: Gastroenterology. 154, 5, p. 1435-1448 14 p.

Research output: Contribution to journal › Journal article › Research › peer-review

55 Citations (Scopus)

HCV Genotype 6a Escape from and Resistance to Velpatasvir, Pibrentasvir, and Sofosbuvir in Robust Infectious Cell Culture Models

Pham, L., Ramirez Almeida, S., Gottwein, J. M., Fahnøe, U., Li, Y., Pedersen, J. & Bukh, J., Jun 2018, In: Gastroenterology. 154, 8, p. 2194-2208.e12

Research output: Contribution to journal › Journal article › Research › peer-review

27 Citations (Scopus)

High density Huh7.5 cell hollow fiber bioreactor culture for high-yield production of hepatitis C virus and studies of antivirals

Pihl, A. F., Offersgaard, A. F., Mathiesen, C. K., Prentoe, J., Fahnøe, U., Krarup, H., Bukh, J. & Gottwein, J. M., 2018, In: Scientific Reports. 8, 1, 13 p., 17505.

Research output: Contribution to journal › Journal article › Research › peer-review

2 Citations (Scopus)

53 Downloads (Pure)

Recombinant hepatitis C virus genotype 5a infectious cell culture systems expressing minimal JFH1 NS5B sequences permit polymerase inhibitor studies

Humes, D., Ramirez, S., Jensen, T. B., Li, Y. P., Gottwein, J. M. & Bukh, J., 2018, In: Virology. 522, p. 177-192 16 p.

Research output: Contribution to journal › Journal article › Research › peer-review

2 Citations (Scopus)

2017

Efficient hepatitis c virus genotype 1b core-NS5A recombinants permit efficacy testing of protease and NS5A inhibitors

Pham, L. V., Ramirez Almeida, S., Carlsen, T. H. R., Li, Y.-P., Gottwein, J. M. & Bukh, J., 2017, In: Antimicrobial Agents and Chemotherapy. 61, 6, 19 p., e00037-17.

Research output: Contribution to journal › Journal article › Research › peer-review

9 Citations (Scopus)

2016

Robust HCV Genotype 3a Infectious Cell Culture System Permits Identification of Escape Variants With Resistance to Sofosbuvir

Ramirez Almeida, S., Mikkelsen, L. S., Gottwein, J. M. & Bukh, J., Nov 2016, In: Gastroenterology. 151, 5, p. 973-985.e2

Research output: Contribution to journal › Journal article › Research › peer-review

52 Citations (Scopus)

2015

Adaptive Mutations Enhance Assembly and Cell-to-Cell Transmission of a High-Titer Hepatitis C Virus Genotype 5a Core-NS2 JFH1-Based Recombinant

Mathiesen, C. K., Prentoe, J., Meredith, L. W., Jensen, T. B., Krarup, H., McKeating, J. A., Gottwein, J. M. & Bukh, J., Aug 2015, In: Journal of Virology. 89, 15, p. 7758-7775 18 p.

Research output: Contribution to journal › Journal article › Research › peer-review

15 Citations (Scopus)

Cell culture system of a hepatitis C genotype 3a and 2a chimera: Patent #: 8,945,584

Gottwein, J. M. (Inventor), Scheel, T. K. H. (Inventor), Eugen-Olsen, J. (Inventor) & Bukh, J. (Inventor), 3 Feb 2015, IPC No. A61K 39/29 (20060101); C12N 15/06 (20060101); C12Q 1/02 (20060101); C07H 21/00 (20060101); C12N 7/02 (20060101); A61K 39/12 (20060101), Patent No. WO2008/125117, 23 Oct 2008, Priority date 11 Apr 2008, Priority No. PCT/DK2008/050083

Research output: Patent

Infectious hepatitis C viruses of genotype 3A and 4A and uses thereof: Patent #: 8,946,398

Gottwein, J. M. (Inventor), Scheel, T. K. H. (Inventor), Purcell, R. E. (Inventor) & Bukh, J. (Inventor), 3 Feb 2015, IPC No. C07H 21/04 (20060101); C07H 21/02 (20060101), Patent No. WO2011/038737, 26 Jul 2012, Priority date 16 Sept 2010, Priority No. PCT/DK2010/050236

Research output: Patent

Substitutions at NS3 Residue 155, 156, or 168 of Hepatitis C Virus Genotypes 2 to 6 Induce Complex Patterns of Protease Inhibitor Resistance

Jensen, S. B., Serre, S. B. N., Humes, D. G., Ramirez Almeida, S., Li, Y.-P., Bukh, J. & Gottwein, J. M., 1 Dec 2015, In: Antimicrobial Agents and Chemotherapy. 59, 12, p. 7426-7436 11 p.

Research output: Contribution to journal › Journal article › Research › peer-review

29 Citations (Scopus)

2014

Differential sensitivity of 5'UTR-NS5A recombinants of hepatitis C virus genotypes 1-6 to protease and NS5A inhibitors

Li, Y.-P., Ramirez, S., Humes, D., Jensen, S. B., Gottwein, J. M. & Bukh, J., Mar 2014, In: Gastroenterology. 146, 3, p. 812-821.e4 14 p.

Research output: Contribution to journal › Journal article › Research › peer-review

48 Citations (Scopus)

Efficient cell culture system for hepatitis C virus genotype 2B: Patent #: 8,663,653

Gottwein, J. M. (Inventor), Knudsen, M. L. (Inventor), Scheel, T. K. H. (Inventor) & Bukh, J. (Inventor), 31 Mar 2014, IPC No. A61K 39/12 (20060101); A61K 39/29 (20060101); C12N 1/20 (20060101); C12N 5/071 (20100101); C12N 15/00 (20060101); C12Q 1/70 (20060101), Patent No. WO2010/017818, 18 Feb 2010, Priority date 24 Jul 2009, Priority No. PCT/DK2009/050189

Research output: Patent

Hepatitis C virus expressing reporter tagged NS5A protein: Patent #: 8,772,022

Gottwein, J. M. (Inventor), Scheel, T. K. H. (Inventor), Bukh, J. (Inventor), Prentø, J. C. (Inventor) & Jensen, T. B. (Inventor), 8 Jul 2014, IPC No. C12N 15/00 (20060101); C12N 5/00 (20060101); C12N 7/00 (20060101); C12N 15/64 (20060101); A61K 39/29 (20060101); C12N 7/01 (20060101), Patent No. WO2010/037403, 8 Apr 2010, Priority date 5 Oct 2009, Priority No. PCT/DK2009/050263

Research output: Patent

Highly efficient infectious cell culture of three hepatitis C virus genotype 2b strains and sensitivity to lead protease, nonstructural protein 5A, and polymerase inhibitors

Ramirez, S., Li, Y.-P., Jensen, S. B., Pedersen, J., Gottwein, J. M. & Bukh, J., Feb 2014, In: Hepatology. 59, 2, p. 395-407 13 p.

Research output: Contribution to journal › Journal article › Research › peer-review

53 Citations (Scopus)

Infectious genotype 1a, 1b, 2a, 2b, 3a, 5a, 6a and 7a hepatitis C virus lacking the hypervariable region 1 (HVR1): Patent #: 8,846,891

Prentø, J. C. (Inventor), Gottwein, J. M. (Inventor), Scheel, T. K. H. (Inventor), Bukh, J. (Inventor) & Jensen, T. B. (Inventor), 30 Sept 2014, IPC No. C07H 21/04 (20060101); C07H 21/02 (20060101), Patent No. WO2010/022727, 4 Mar 2010, Priority date 7 Aug 2009, Priority No. PCT/DK2009/050197

Research output: Patent

Production and characterization of high-titer serum-free cell culture grown hepatitis C virus particles of genotype 1-6

Mathiesen, C. K., Jensen, T. B., Prentoe, J., Krarup, H., Nicosia, A., Law, M., Bukh, J. & Gottwein, J. M., Jun 2014, In: Virology. 458-459, p. 190-208 19 p.

Research output: Contribution to journal › Journal article › Research › peer-review

10 Citations (Scopus)

2013

Adapted J6/JFH1-based Hepatitis C virus recombinants with genotype-specific NS4A show similar efficacies against lead protease inhibitors, alpha interferon, and a putative NS4A inhibitor

Gottwein, J. M., Jensen, S. B., Serre, S. B. N., Ghanem, L., Scheel, T. K. H., Jensen, T. B., Krarup, H., Uzcategui, N., Mikkelsen, L. S. & Bukh, J., Dec 2013, In: Antimicrobial Agents and Chemotherapy. 57, 12, p. 6034-49 16 p.

Research output: Contribution to journal › Journal article › Research › peer-review

12 Citations (Scopus)

Adaptive mutations allow establishment of JFH1-based cell culture systems for hepatitis C virus genotype 4A: Patent #: 8,454,974

Scheel, T. K. H. (Inventor), Gottwein, J. M. (Inventor), Eugen-Olsen, J. (Inventor) & Bukh, J. (Inventor), 4 Jun 2013, IPC No. A61K 39/29 (20060101); C07H 21/00 (20060101); C12N 15/06 (20060101); C12N 7/02 (20060101); C12Q 1/02 (20060101), Patent No. WO2008/125119, 23 Oct 2008, Priority date 11 Apr 2008, Priority No. PCT/DK2008/050085

Research output: Patent

Analysis of hepatitis C virus core/NS5A protein co-localization using novel cell culture systems expressing core-NS2 and NS5A of genotypes 1-7

Galli, A., Scheel, T. K. H., Prentoe, J. C., Mikkelsen, L. S., Gottwein, J. M. & Bukh, J., Oct 2013, In: The Journal of general virology. 94, Pt 10, p. 2221-35 15 p.

Research output: Contribution to journal › Journal article › Research › peer-review

14 Citations (Scopus)

Combination treatment with hepatitis C virus protease and NS5A inhibitors is effective against recombinant genotype 1a, 2a, and 3a viruses

Gottwein, J. M., Jensen, S. B., Li, Y.-P., Ghanem, L., Scheel, T. K. H., Serre, S. B. N., Mikkelsen, L. & Bukh, J., Mar 2013, In: Antimicrobial Agents and Chemotherapy. 57, 3, p. 1291-303 13 p.

Research output: Contribution to journal › Journal article › Research › peer-review

29 Citations (Scopus)

Efficient cell culture system for hepatitis C virus genotype 1a and 1b: Patent #: 8,563,706

Scheel, T. K. H. (Inventor), Gottwein, J. M. (Inventor), Prentø, J. C. (Inventor), Jensen, T. B. (Inventor) & Bukh, J. (Inventor), 22 Oct 2013, IPC No. C07H 21/00 (20060101); A61K 39/29 (20060101); A61K 39/12 (20060101); A61K 39/295 (20060101); C12N 7/00 (20060101), Patent No. WO2009/080053, 2 Jul 2009, Priority date 19 Dec 2008, Priority No. PCT/DK2008/050333

Research output: Patent

Efficient cell culture system for hepatitis C virus genotype 5A: Patent #: 8,618,275

Jensen, T. B. (Inventor), Gottwein, J. M. (Inventor), Scheel, T. K. H. (Inventor), Eugen-Olsen, J. (Inventor) & Bukh, J. (Inventor), 31 Dec 2013, IPC No. C07H 21/00 (20060101); C12N 7/00 (20060101); A61K 39/295 (20060101); A61K 39/12 (20060101); A61K 39/29 (20060101), Patent No. WO2008/141651, 27 Nov 2008, Priority date 19 May 2008, Priority No. PCT/DK2008/050113

Research output: Patent

Efficient cell culture system for hepatitis C virus genotype 6A: Patent #: 8,569,472

Gottwein, J. M. (Inventor), Scheel, T. K. H. (Inventor), Jensen, T. B. (Inventor) & Bukh, J. (Inventor), 29 Oct 2013, IPC No. C07H 21/00 (20060101); C12N 7/00 (20060101); A61K 39/295 (20060101); A61K 39/12 (20060101); A61K 39/29 (20060101), Patent No. WO2009/080052, 2 Jul 2009, Priority date 19 Dec 2008, Priority No. PCT/DK2008/050332

Research output: Patent

Efficient cell culture system for hepatitis C virus genotype 7a: Patent #: 8,506,969

Gottwein, J. M. (Inventor), Scheel, T. K. H. (Inventor), Jensen, T. B. (Inventor) & Bukh, J. (Inventor), 13 Aug 2013, IPC No. A61K 39/12 (20060101); A61K 39/29 (20060101); C12Q 1/70 (20060101); C12N 15/00 (20060101); C12N 5/071 (20100101); C12N 1/20 (20060101), Patent No. WO2010/017819, 18 Feb 2010, Priority date 31 Jul 2009, Priority No. PCT/DK2009/050193

Research output: Patent

Identification of alpha interferon-induced envelope mutations of hepatitis C virus in vitro associated with increased viral fitness and interferon resistance

Serre, S. B. N., Krarup, H. B., Bukh, J. & Gottwein, J. M., Dec 2013, In: Journal of Virology. 87, 23, p. 12776-93 18 p.

Research output: Contribution to journal › Journal article › Research › peer-review

19 Citations (Scopus)

Neutralization resistance of hepatitis C virus can be overcome by recombinant human monoclonal antibodies

Pedersen, J. L., Carlsen, T. H. R., Prentoe, J., Ramirez, S., Jensen, T. B., Forns, X., Alter, H., Foung, S. K. H., Law, M., Gottwein, J., Weis, N. & Bukh, J., Nov 2013, In: Hepatology. 58, 5, p. 1587-1597 11 p.

Research output: Contribution to journal › Journal article › Research › peer-review

31 Citations (Scopus)

Productive homologous and non-homologous recombination of hepatitis C virus in cell culture

Scheel, T. K. H., Galli, A., Li, Y.-P., Mikkelsen, L. S., Gottwein, J. M. & Bukh, J., Mar 2013, In: P L o S Pathogens. 9, 3, p. 1-12 13 p., e1003228.

Research output: Contribution to journal › Journal article › Research › peer-review

28 Citations (Scopus)

Viral hepatitis: Cell-culture-derived HCV--a promising vaccine antigen

Gottwein, J. M. & Bukh, J., 1 Sept 2013, In: Nature Reviews. Gastroenterology & Hepatology. 10, 9, p. 508-9 2 p.

Research output: Contribution to journal › Journal article › Research › peer-review

9 Citations (Scopus)

2012

Analysis of functional differences between hepatitis C virus NS5A of genotypes 1-7 in infectious cell culture systems

Scheel, T. K. H., Prentoe, J., Carlsen, T. H. R., Mikkelsen, L. S., Gottwein, J. M. & Bukh, J., May 2012, In: P L o S Pathogens. 8, 5, p. 1-16 17 p., e1002696.

Research output: Contribution to journal › Journal article › Research › peer-review

33 Citations (Scopus)

Efficient replication of genotype 3a and 4a hepatitis C virus replicons in human hepatoma cells

Saeed, M., Scheel, T. K. H., Gottwein, J. M., Marukian, S., Dustin, L. B., Bukh, J. & Rice, C. M., Oct 2012, In: Antimicrobial Agents and Chemotherapy. 56, 10, p. 5365-73 9 p.

Research output: Contribution to journal › Journal article › Research › peer-review

99 Citations (Scopus)

Highly efficient full-length hepatitis C virus genotype 1 (strain TN) infectious culture system

Li, Y.-P., Ramirez, S., Jensen, S. B., Purcell, R. H., Gottwein, J. M. & Bukh, J., 27 Nov 2012, In: Proceedings of the National Academy of Sciences of the United States of America. 109, 48, p. 19757-62 6 p.

Research output: Contribution to journal › Journal article › Research › peer-review

88 Citations (Scopus)

Robust full-length hepatitis C virus genotype 2a and 2b infectious cultures using mutations identified by a systematic approach applicable to patient strains

Li, Y.-P., Ramirez, S., Gottwein, J. M., Scheel, T. K. H., Mikkelsen, L., Purcell, R. H. & Bukh, J., 1 May 2012, In: Proceedings of the National Academy of Sciences USA (PNAS). 109, 18, p. E1101-E1110 10 p.

Research output: Contribution to journal › Journal article › Research › peer-review

69 Citations (Scopus)

2010

Novel infectious cDNA clones of hepatitis C virus genotype 3a (strain S52) and 4a (strain ED43): genetic analyses and in vivo pathogenesis studies

Gottwein, J., Scheel, T., Callendret, B., Li, Y., Eccleston, H. B., Engle, R. E., Govindarajan, S., Satterfield, W., Purcell, R. H., Walker, C. M. & Bukh, J., May 2010, In: Journal of Virology. 84, 10, p. 5277-93 17 p.

Research output: Contribution to journal › Journal article › Research › peer-review

100 Citations (Scopus)

2009

DELETION OF HYPERVARIABLE REGION 1 (HVR1) OF E2 FROM HCV GENOTYPES 1-6 HAS DIFFERENTIAL EFFECT ON INFECTIVITY IN VITRO AND CAUSES INCREASED SUSCEPTIBILITY TO NEUTRALIZATION

Prentoe, J., Gottwein, J. M., Scheel, T. K. H., Jensen, T. B., Serre, S. & Bukh, J., 2009, In: Journal of Hepatology. 50, p. S34-S34

Research output: Contribution to journal › Journal article › Research › peer-review

Development and characterization of hepatitis C virus genotype 1-7 cell culture systems: role of CD81 and scavenger receptor class B type I and effect of antiviral drugs

Gottwein, J. M., Scheel, T. K. H., Jensen, T. B., Lademann, J. B., Prentoe, J. C., Knudsen, M. L., Hoegh, A. M. & Bukh, J., 2009, In: Hepatology. 49, 2, p. 364-77 13 p.

Research output: Contribution to journal › Journal article › Research › peer-review

281 Citations (Scopus)

MONOCISTRONIC HEPATITIS C REPORTER VIRUS RECOMBINANTS OF ALL MAJOR GENOTYPES EXPRESSING ENHANCED GREEN FLUORESCENT PROTEIN TAGGED NS5A PROTEIN

Gottwein, J. M., Lademann, J. B., Scheel, T. K. H., Jensen, T. B., Prentoe, J. C. & Bukh, J., 2009, In: Journal of Hepatology. 50, p. S33-S33

Research output: Contribution to journal › Journal article › Research › peer-review

2008

Cutting the gordian knot-development and biological relevance of hepatitis C virus cell culture systems.

Gottwein, J. M. & Bukh, J., 2008, In: Advances in Virus Research. 71, p. 51-133 82 p.

Research output: Contribution to journal › Journal article › Research › peer-review

83 Citations (Scopus)

Development of JFH1-based cell culture systems for hepatitis C virus genotype 4a and evidence for cross-genotype neutralization

Scheel, T. K. H., Gottwein, J. M., Jensen, T. B., Prentø, J. C., Høegh, A. M., Alter, H. J., Eugen-Olsen, J. & Bukh, J., 2008, In: Proceedings of the National Academy of Science of the United States of America. 105, 3, p. 997-1002 5 p.

Research output: Contribution to journal › Journal article › Research › peer-review

149 Citations (Scopus)

Highly Efficient JFH1-Based Cell-Culture System for Hepatitis C Virus Genotype 5a: Failure of Homologous Neutralizing-Antibody Treatment to Control Infection

Jensen, T. B., Gottwein, J. M., Scheel, T. K. H., Høgh, A. M., Eugen-Olsen, J. & Bukh, J., 2008, In: Journal of Infectious Diseases. 198, 12, p. 1756-1765 9 p.

Research output: Contribution to journal › Journal article › Research › peer-review

87 Citations (Scopus)

Judith Margarete Gottwein

Research output

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