Efficient cell culture system for hepatitis C virus genotype 2B: Patent #: 8,663,653

Judith Margarete Gottwein; Maria Lisa Knudsen; Troels Kasper Hoyer  Scheel; Jens Bukh

Efficient cell culture system for hepatitis C virus genotype 2B: Patent #: 8,663,653

Judith Margarete Gottwein (Inventor), Maria Lisa Knudsen (Inventor), Troels Kasper Hoyer Scheel (Inventor), Jens Bukh (Inventor)

Abstract

The present inventors developed hepatitis C virus 2b/2a intergenotypic recombinants in which the JFH1 structural genes (Core, E1 and E2), p7 and the complete NS2 were replaced by the corresponding genes of the genotype 2b reference strain J8. Sequence analysis of recovered 2b/2a recombinants from 2 transfection experiments revealed that 2b/2a was genetically stable. Conclusion: The developed 2b/2a viruses provide a robust in vitro tool for research in HCV genotype 2b, including vaccine studies and functional analysis.

Original language	English
IPC	A61K 39/12 (20060101); A61K 39/29 (20060101); C12N 1/20 (20060101); C12N 5/071 (20100101); C12N 15/00 (20060101); C12Q 1/70 (20060101)
Patent number	WO2010/017818
Filing date	18/02/2010
Country/Territory	Denmark
Priority date	24/07/2009
Priority number	PCT/DK2009/050189
Publication status	Published - 31 Mar 2014

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title = "Efficient cell culture system for hepatitis C virus genotype 2B: Patent #: 8,663,653 ",

abstract = "The present inventors developed hepatitis C virus 2b/2a intergenotypic recombinants in which the JFH1 structural genes (Core, E1 and E2), p7 and the complete NS2 were replaced by the corresponding genes of the genotype 2b reference strain J8. Sequence analysis of recovered 2b/2a recombinants from 2 transfection experiments revealed that 2b/2a was genetically stable. Conclusion: The developed 2b/2a viruses provide a robust in vitro tool for research in HCV genotype 2b, including vaccine studies and functional analysis.",

author = "Gottwein, {Judith Margarete} and Knudsen, {Maria Lisa} and Scheel, {Troels Kasper Hoyer} and Jens Bukh",

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language = "English",

type = "Patent",

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T1 - Efficient cell culture system for hepatitis C virus genotype 2B

T2 - Patent #: 8,663,653

AU - Gottwein, Judith Margarete

AU - Knudsen, Maria Lisa

AU - Scheel, Troels Kasper Hoyer

AU - Bukh, Jens

PY - 2014/3/31

Y1 - 2014/3/31

N2 - The present inventors developed hepatitis C virus 2b/2a intergenotypic recombinants in which the JFH1 structural genes (Core, E1 and E2), p7 and the complete NS2 were replaced by the corresponding genes of the genotype 2b reference strain J8. Sequence analysis of recovered 2b/2a recombinants from 2 transfection experiments revealed that 2b/2a was genetically stable. Conclusion: The developed 2b/2a viruses provide a robust in vitro tool for research in HCV genotype 2b, including vaccine studies and functional analysis.

AB - The present inventors developed hepatitis C virus 2b/2a intergenotypic recombinants in which the JFH1 structural genes (Core, E1 and E2), p7 and the complete NS2 were replaced by the corresponding genes of the genotype 2b reference strain J8. Sequence analysis of recovered 2b/2a recombinants from 2 transfection experiments revealed that 2b/2a was genetically stable. Conclusion: The developed 2b/2a viruses provide a robust in vitro tool for research in HCV genotype 2b, including vaccine studies and functional analysis.

M3 - Patent

M1 - WO2010/017818

Y2 - 2010/02/18

ER -

Efficient cell culture system for hepatitis C virus genotype 2B: Patent #: 8,663,653

Abstract

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