Efficient cell culture system for hepatitis C virus genotype 7a: Patent #: 8,506,969

Judith Margarete Gottwein; Troels Kasper Hoyer  Scheel; Tanja Bertelsen Jensen; Jens Bukh

Efficient cell culture system for hepatitis C virus genotype 7a: Patent #: 8,506,969

Judith Margarete Gottwein (Inventor), Troels Kasper Hoyer Scheel (Inventor), Tanja Bertelsen Jensen (Inventor), Jens Bukh (Inventor)

Department of Immunology and Microbiology

Abstract

Genotype 7a has been identified recently, thus not much is known about the biology of this new, major HCV genotype. The present inventors developed hepatitis C virus 7a/2a intergenotypic recombinants in which the JFH1 structural genes (Core, E1 and E2), p7 and the complete NS2 were replaced by the corresponding genes of the genotype 7a strain QC69 and characterized them in Huh7.5 cells. Sequence analysis of 7a/JFH1 recombinants recovered after viral passage in Huh7.5 cells following 4 independent transfection experiments revealed adaptive mutations in Core, E2, NS2, NS5A and NS5B. In reverse genetic studies the importance of these mutations for improved growth kinetics was shown. Adapted 7a/JFH1 viruses showed growth kinetics, infectivity and RNA titers comparable to a previously developed 3a/JFH1 reference virus. Conclusion: The developed 7a/JFH1 viruses provide a robust in vitro tool for research in HCV genotype 7, including vaccine studies and functional analyses

Original language	English
IPC	A61K 39/12 (20060101); A61K 39/29 (20060101); C12Q 1/70 (20060101); C12N 15/00 (20060101); C12N 5/071 (20100101); C12N 1/20 (20060101)
Patent number	WO2010/017819
Filing date	18/02/2010
Country/Territory	Denmark
Priority date	31/07/2009
Priority number	PCT/DK2009/050193
Publication status	Published - 13 Aug 2013

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title = "Efficient cell culture system for hepatitis C virus genotype 7a: Patent #: 8,506,969 ",

abstract = "Genotype 7a has been identified recently, thus not much is known about the biology of this new, major HCV genotype. The present inventors developed hepatitis C virus 7a/2a intergenotypic recombinants in which the JFH1 structural genes (Core, E1 and E2), p7 and the complete NS2 were replaced by the corresponding genes of the genotype 7a strain QC69 and characterized them in Huh7.5 cells. Sequence analysis of 7a/JFH1 recombinants recovered after viral passage in Huh7.5 cells following 4 independent transfection experiments revealed adaptive mutations in Core, E2, NS2, NS5A and NS5B. In reverse genetic studies the importance of these mutations for improved growth kinetics was shown. Adapted 7a/JFH1 viruses showed growth kinetics, infectivity and RNA titers comparable to a previously developed 3a/JFH1 reference virus. Conclusion: The developed 7a/JFH1 viruses provide a robust in vitro tool for research in HCV genotype 7, including vaccine studies and functional analyses",

author = "Gottwein, {Judith Margarete} and Scheel, {Troels Kasper Hoyer} and Jensen, {Tanja Bertelsen} and Jens Bukh",

year = "2013",

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language = "English",

type = "Patent",

note = "WO2010/017819; A61K 39/12 (20060101); A61K 39/29 (20060101); C12Q 1/70 (20060101); C12N 15/00 (20060101); C12N 5/071 (20100101); C12N 1/20 (20060101)",

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TY - PAT

T1 - Efficient cell culture system for hepatitis C virus genotype 7a

T2 - Patent #: 8,506,969

AU - Gottwein, Judith Margarete

AU - Scheel, Troels Kasper Hoyer

AU - Jensen, Tanja Bertelsen

AU - Bukh, Jens

PY - 2013/8/13

Y1 - 2013/8/13

N2 - Genotype 7a has been identified recently, thus not much is known about the biology of this new, major HCV genotype. The present inventors developed hepatitis C virus 7a/2a intergenotypic recombinants in which the JFH1 structural genes (Core, E1 and E2), p7 and the complete NS2 were replaced by the corresponding genes of the genotype 7a strain QC69 and characterized them in Huh7.5 cells. Sequence analysis of 7a/JFH1 recombinants recovered after viral passage in Huh7.5 cells following 4 independent transfection experiments revealed adaptive mutations in Core, E2, NS2, NS5A and NS5B. In reverse genetic studies the importance of these mutations for improved growth kinetics was shown. Adapted 7a/JFH1 viruses showed growth kinetics, infectivity and RNA titers comparable to a previously developed 3a/JFH1 reference virus. Conclusion: The developed 7a/JFH1 viruses provide a robust in vitro tool for research in HCV genotype 7, including vaccine studies and functional analyses

AB - Genotype 7a has been identified recently, thus not much is known about the biology of this new, major HCV genotype. The present inventors developed hepatitis C virus 7a/2a intergenotypic recombinants in which the JFH1 structural genes (Core, E1 and E2), p7 and the complete NS2 were replaced by the corresponding genes of the genotype 7a strain QC69 and characterized them in Huh7.5 cells. Sequence analysis of 7a/JFH1 recombinants recovered after viral passage in Huh7.5 cells following 4 independent transfection experiments revealed adaptive mutations in Core, E2, NS2, NS5A and NS5B. In reverse genetic studies the importance of these mutations for improved growth kinetics was shown. Adapted 7a/JFH1 viruses showed growth kinetics, infectivity and RNA titers comparable to a previously developed 3a/JFH1 reference virus. Conclusion: The developed 7a/JFH1 viruses provide a robust in vitro tool for research in HCV genotype 7, including vaccine studies and functional analyses

M3 - Patent

M1 - WO2010/017819

Y2 - 2010/02/18

ER -

Efficient cell culture system for hepatitis C virus genotype 7a: Patent #: 8,506,969

Abstract

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