Hepatitis C virus expressing reporter tagged NS5A protein: Patent #: 8,772,022

Judith Margarete Gottwein; Troels Kasper Hoyer  Scheel; Jens Bukh; Jannick Cornelius Prentø; Tanja Bertelsen Jensen

Hepatitis C virus expressing reporter tagged NS5A protein: Patent #: 8,772,022

Judith Margarete Gottwein (Inventor), Troels Kasper Hoyer Scheel (Inventor), Jens Bukh (Inventor), Jannick Cornelius Prentø (Inventor), Tanja Bertelsen Jensen (Inventor)

Abstract

Hepatitis C reporter viruses containing Core through NS2 of prototype isolates of all major HCV genotypes and the remaining genes of isolate JFH1, by insertion of reporter genes in domain III of HCV NS5A were developed. A deletion upstream of the inserted reporter gene sequence conferred favorable growth kinetics in Huh7.5 cells to these viruses. These reporter viruses can be used for high throughput analysis of drug and vaccine candidates as well as patient samples. JFH1-based intergenotypic recombinants with genotype specific homotypic 5'UTR, or heterotypic 5'UTR (either of genotype 1a (strain H77) or of genotype 3a (strain S52)) were also developed. The present inventors additionally developed J6/JFH1 recombinants with the 5'UTR of genotypes 1-6. These recombinants with different 5'UTRs are a useful to study the function of the 5'UTR in a genotype specific manner

Original language	English
IPC	C12N 15/00 (20060101); C12N 5/00 (20060101); C12N 7/00 (20060101); C12N 15/64 (20060101); A61K 39/29 (20060101); C12N 7/01 (20060101)
Patent number	WO2010/037403
Filing date	08/04/2010
Country/Territory	Denmark
Priority date	05/10/2009
Priority number	PCT/DK2009/050263
Publication status	Published - 8 Jul 2014

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title = "Hepatitis C virus expressing reporter tagged NS5A protein: Patent #: 8,772,022 ",

abstract = "Hepatitis C reporter viruses containing Core through NS2 of prototype isolates of all major HCV genotypes and the remaining genes of isolate JFH1, by insertion of reporter genes in domain III of HCV NS5A were developed. A deletion upstream of the inserted reporter gene sequence conferred favorable growth kinetics in Huh7.5 cells to these viruses. These reporter viruses can be used for high throughput analysis of drug and vaccine candidates as well as patient samples. JFH1-based intergenotypic recombinants with genotype specific homotypic 5'UTR, or heterotypic 5'UTR (either of genotype 1a (strain H77) or of genotype 3a (strain S52)) were also developed. The present inventors additionally developed J6/JFH1 recombinants with the 5'UTR of genotypes 1-6. These recombinants with different 5'UTRs are a useful to study the function of the 5'UTR in a genotype specific manner",

author = "Gottwein, {Judith Margarete} and Scheel, {Troels Kasper Hoyer} and Jens Bukh and Prent{\o}, {Jannick Cornelius} and Jensen, {Tanja Bertelsen}",

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type = "Patent",

note = "WO2010/037403; C12N 15/00 (20060101); C12N 5/00 (20060101); C12N 7/00 (20060101); C12N 15/64 (20060101); A61K 39/29 (20060101); C12N 7/01 (20060101)",

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T1 - Hepatitis C virus expressing reporter tagged NS5A protein

T2 - Patent #: 8,772,022

AU - Gottwein, Judith Margarete

AU - Scheel, Troels Kasper Hoyer

AU - Bukh, Jens

AU - Prentø, Jannick Cornelius

AU - Jensen, Tanja Bertelsen

PY - 2014/7/8

Y1 - 2014/7/8

N2 - Hepatitis C reporter viruses containing Core through NS2 of prototype isolates of all major HCV genotypes and the remaining genes of isolate JFH1, by insertion of reporter genes in domain III of HCV NS5A were developed. A deletion upstream of the inserted reporter gene sequence conferred favorable growth kinetics in Huh7.5 cells to these viruses. These reporter viruses can be used for high throughput analysis of drug and vaccine candidates as well as patient samples. JFH1-based intergenotypic recombinants with genotype specific homotypic 5'UTR, or heterotypic 5'UTR (either of genotype 1a (strain H77) or of genotype 3a (strain S52)) were also developed. The present inventors additionally developed J6/JFH1 recombinants with the 5'UTR of genotypes 1-6. These recombinants with different 5'UTRs are a useful to study the function of the 5'UTR in a genotype specific manner

AB - Hepatitis C reporter viruses containing Core through NS2 of prototype isolates of all major HCV genotypes and the remaining genes of isolate JFH1, by insertion of reporter genes in domain III of HCV NS5A were developed. A deletion upstream of the inserted reporter gene sequence conferred favorable growth kinetics in Huh7.5 cells to these viruses. These reporter viruses can be used for high throughput analysis of drug and vaccine candidates as well as patient samples. JFH1-based intergenotypic recombinants with genotype specific homotypic 5'UTR, or heterotypic 5'UTR (either of genotype 1a (strain H77) or of genotype 3a (strain S52)) were also developed. The present inventors additionally developed J6/JFH1 recombinants with the 5'UTR of genotypes 1-6. These recombinants with different 5'UTRs are a useful to study the function of the 5'UTR in a genotype specific manner

M3 - Patent

M1 - WO2010/037403

Y2 - 2010/04/08

ER -

Hepatitis C virus expressing reporter tagged NS5A protein: Patent #: 8,772,022

Abstract

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