CV

 CURRICULUM VITAE

Bishwanath Kumar Chourasia, Ph.D                                                                

Email: [email protected] 

Mobile: +4550221022                                                                       

[email protected]

RESEARCH EXPERIENCE

1. Post-Doctoral Fellow – Centre for Medical Parasitology at Department of International Health Immunology and Microbiology, Statens Serum Institut, University of Copenhagen, Denmark.

Tenure: November, 2016 - present

Project: Process development of recombinant fusion protein and formulation studies. 

2. Research Associate - International Center for Genetic Engineering and Biotechnology, New Delhi

Tenure: October 2012 – November 2016.

Project: Characterization of Plasmodium falciparum merozoite surface complex for the development of a multicomponent malaria vaccine.

3. ICMR Senior Research Fellowship – Department of Microbiology, All India Institute of Medical Sciences, New Delhi

Tenure: January 2009 - July 2012.

Project: Development of immunodiagnostic assay for field use for detection of Mycoplasma pneumoniae.

4. Senior Research Fellow - Department of Microbiology, All India Institute of Medical Sciences, New Delhi

Tenure:  October 2005 - July 2008.

Project: Cloning and expression of Mycoplasma pneumoniae P1 gene and its fragments to study their role in cytadherence.

5. Junior Research Fellow - Department of Ocular Microbiology, All India Institute of Medical Sciences, New Delhi

Tenure: May 2003 - September 2005.

Project: Development of novel molecular diagnostic test for eye disease.  

ACADEMIC QUALIFICATIONS:

Ph.D. (2007-2012)

Thesis Title: Cloning and characterization of cytadherence region (s) of Mycoplasma pneumoniae P1 gene.

Research Supervisor: Prof. Rama Chaudhry, Department of Microbiology, AIIMS, New Delhi.

Master of Science in Biotechnology (2000-2002), Bundelkhand University, Jhansi, UP; 70.14%

• M.Sc. dissertation: January 2002-June 2002

Studies on radioprotection of compounds using yeast, “Saccharomyces cerevisiae”.

Research Supervisor:  Prof. K Pasupathy (Scientist ‘G’), Radiation Biology Division; Bhabha Atomic Research Centre, Mumbai.  

Bachelor of Science in Biotechnology (1997-2000), Patna University, Patna, Bihar; 60.13%

• Project on Mycotoxin (2 months)  

   Mycotoxin isolation, extraction of toxin metabolites, purification and effect of culture filtrate on seed germination (maize or Gram).

   Research Supervisor: Choudhary Sharfuddin, Department of Microbiology and Plant Pathology, Patna University, Patna. 

• Training (one week)

   Production and Quality Control of the Bacterial & Viral Vaccine.

   Guide: Dr. G. M Kumar, Institute of Animal Health & Production, Patna.

• Training (one week)

   Demonstration of clinical laboratory techniques including automated procedures. 

   Guide: Dr.Sandeep Sen, Sen Medical Research Centre, Patna.    

• Training (one week)

   Quantitative detection of blood hormones 

   Guide:Dr. Akhouri R.S. Sinha, HOD, Department of Biochemistry, Patna Medical College, Patna. 

TECHNICAL SKILLS 

Microbiology

Preparation of suitable liquid/solid synthetic media, Isolation of aerobic and anaerobic bacteria from different sources, biochemical characterization of microbes, maintenance of bacterial strains, M. pneumoniae standard strain F.H lieu (NCTC London), Haploid strain of S. cerevisiae, Wild type ATCC 3177 and rad 52. Determination of cell survival after exposure of Gamma radiation to cell suspension.

Animal Cell Culture Technology

Primary cell culture (HEp-2 strains, ATCC, CCL-23), passaging, cell line maintenance, Trypsinization and basic operation in Inverted Microscope. 

Molecular Biology

DNA-RNA isolation from clinical sample, microbial and Plasmodium falciparum, PCR, cDNA preparation, Cloning, Protein expression & purification, Ultracentrifugation, Southern blotting, Immunofluorescence Microscopy, Enzyme assays, SDS PAGE, Gel electrophoresis, Gradient centrifugation, ELISA, Western blotting, Bioplex assay, protein-protein interaction by Surface Plasmon Resonance (SPR), Production of antibodies against recombinant proteins in Rabbit and Mice.

Instrumentation Skills

FPLC, Acta Express, Fermenter, UV spectrophotometer, Gel Documentation System and all other basic instruments.

Confocal Microscop   

Immunostaining of fixed and liquid cultures, Data analysis and image processing

PUBLICATIONS

1. Nemavarkar P, Chourasia B. K and Pasupathy K. Evaluation of radioprotective action of compounds using Saccharomyces cerevisiae. Journal of Environmental Pathology Toxicology & Oncology 2004; 23:145-151.
2. P. S. Nemavarkar, B. K. Chourasia and K. Pasupathy. Detection of gamma-irradiation induced DNA damage and radioprotection of compounds in Yeast using comet assay. Journal of Radiation Research2004; 45(2):169-174.
3. Irum Tabassum, Rama Chaudhry, Bishwanath Kumar Chourasia, Pawan Malhotra. Identification of an N-terminal 27 kDa fragment of Mycoplasma pneumoniae P116 protein as specific immunogen in M. pneumoniae infections. BMC Infect Dis. 2010 Dec 13;10:350. 
4. Rama Chaudhry, Anupam Das, Premlatha MM, Aashish Chaudhry, Bishwanath Kumar Chourasia, Dinesh S Chandel, A. B Dey. Serological and molecular approaches for diagnosis of leptospirosis in a Tertiary Care Hospital in North India: A 10-year study. Indian J Med Res 137, April 2013, pp 785-790.
5. Bishwanath Kumar Chourasia, Pawan Malhotra, Rama Chaudhry. Delineation of Immunodominant and Cytadherence Segment(s) of Mycoplasma pneumoniae P1 Gene. BMC Microbiol. 2014 Apr 28; 14(1):108.
6. Chaudhry R, Saigal K, Bahadur T, Kant K, Chourasia B, Gupta N. Varied presentations of leptospirosis: experience from a tertiary care hospital in north India. Trop Doct. 2017 Apr;47(2):128-132.
7. Susheel K. Singh, Will Roeffen, Ulrik H. Mistarz, Bishwanath Kumar Chourasia, Fen Yang, Kasper D. Rand, Robert W. Sauerwein, Michael Theisen.  Construct design, fermentation, and purification of Plasmodium falciparum 48/45 R0.6C subunit protein produced in Lactococcus lactis as candidate vaccine. Microbial Cell Factories (2017)16:97. 
8. Prem Prakash, Mohammad Zeeshan, Ekta Saini, Azhar Muneer, Sachin Khurana, Bishwanath Chourasia, Arunaditya Deshmukh, Inderjeet Kaur, Surabhi Dabral, Niharika Singh, Zille Anam, Ayushi Chaurasiya, Shikha Kaushik, Pradeep Dahiya, Md. Kalamuddin, Jitendra Thakur, Asif Mohmmed, Anand Ranganathan, and Pawan Malhotra. Human Cyclophilin B forms part of a multi-protein complex during erythrocyte invasion by Plasmodium falciparum. Nature Communication 8: 1548, November 2017; DOI: 10.1038/s41467-017-01638-6.
9. Gourab Paul, Arunaditya Deshmukh, Bishwanath Kumar Chourasia, Md. Kalamuddin, Ashutosh Panda, Susheel Kumar Singh, Pneet K Gupta, Asif Mohmmed, Virender S Chauhan, Michael Theisen, Pawan Malhotra. Protein-protein interaction studies reveal the Plasmodium falciparum Merozoite surface protein-1 region involved in complex formation that binds to human erythrocytes. Biochemical Journal; March 2018; DOI: 10.1042/BCJ20180017.
10. Arunaditya Deshmukh, Bishwanath Kumar Chourasia, Sonali Mehrotra,Ikhlaq Hussain, Gourab Paul, Ashutosh Panda, Inderjeet Kaur, Susheel Kumar Singh, Sumit Rathore, Aparup Das, Priya Gupta, Mohammad Kalamuddin, S.K. Ghakar, Asif Mohmmed, Michael Theisen, Pawan Malhotra. The Nterminal Plasmodium falciparum merozoite surface protein 3, forms a ligand complex on the merozoite surface and elicits natural antibodies relating to clinical protection (Manuscript accepted in Infection and Immunity, March 2018). 
11. Susheel K. Singh, Régis Wendpayangde Tiendrebeogo, Bishwanath  Kumar Chourasia, Ikhlaq Hussain Kana, Subhash Singh, Michael Theisen. Lactococcus lactis provides an efficient platform for production of disulfide-rich recombinant proteins from Plasmodium falciparum (Manuscript accepted in Microbial Cell Factories, March 2018)

PUBLICATIONS UNDER REVIEW/COMMUNICATED 

1. Shweta Sharma, Gautam Kumar, Sumit Rathore, Bishwanath Kumar Chourasia, Jhalak Singhal, Arunaditya Deshmukh, Mohmmed Kalamuddin, Shreyansh Tatiya, Khushboo Rawat, Mohit Vashitha, Rajan Pandey, Dinesh Gupta, Arvind Sahu, Asif Mohmmed, Krishnamurthy Natarajan, Pawan Malhotra. Plasmodium falciparum complement control protein 1 modulates innate immune responses and regulates the diseases progression at asexual blood stages (under Communication in Journal of Immunology).
2. Bishwanath Kumar Chourasia, Arunaditya Deshmukh, Sonali Mehrotra,  Sumit Rathore, Ashutosh Panda, Inderjeet Kaur, Michael Thiesen, Asif Mohmmed, Pawan Malhotra. PfCLAG9 associates with rhoptry proteins to form a complex and is involved in binding to human erythrocytes (under communication in PLOS ONE).
3. Bishwanath Kumar Chourasia, Arunaditya Deshmukh, Sonali Mehrotra, Akshay Kumar Ganguly, Sumit Rathore, shailesh Bajpai, Neel Sarovar Bhavesh, Ali Salanti, Asif Mohamad, Pawan Malhotra. The role of cytoadherence linked asexual gene 9(CLAG9) of Plasmodium falciparum in the trafficking of var, aPfEMP1s onto the IRBC surface (under communication in Journal of Molecular Biology).  

CHAPTER

1. Rama Chaudhry, Bishwanath Kumar Chourasia, Anupam Das. 2011. Molecular detection of Mycoplasma. In Taylor and Francis (ed), Molecular detection of Bacterial Pathogens. Don Liu university of Michigan USA.   

PATENT 

1. Rama Chaudhry, Pawan Malhotra, Bishwanath Kumar Chourasia. Development of immunoassay based on recombinant Mycoplasma pneumoniae P1 fragments (Patent application no. 770/DEL/2012).

WORKSHOP  

1. Bishwanath Kumar Chourasia and Sutikshan Sharma. Demonstration of PCR and cloning of PCR product into pGEM-T Easy vector. VIII Annual Conference-Cum-Workshop-CME on Mycoplasma InfectionsEconomic Impacts & New Challenges of Indian Association of Mycoplasmologists; Department of Microbiology, S. N Medical College, Agra, 5^th to 6^th October, 2007.

POSTER PRESENTED

1. B. K. Chourasia, R. Chaudhry, P. Malhotra, Cloning and expression of Mycoplasma pneumoniae P1 gene fragments and its role in cytadherence. Dr. R. Sengupta and Dr. A.M. Saoji Memorial Prize for Best paper in Immunology.  XXXIII National Conference of Indian Association of Medical Microbiologists, JSS Medical College, Mysore, India from 3^rd to 8^th November, 2009.
2. B. K. Chourasia, Chaudhry R, Malhotra P. Mapping of immunodominant and cytadherence segment (s) of Mycoplasma pneumoniae P1 gene which was awarded The Best Free Poster Award. MICRO-D-CON 2011, Indian Association of Medical Microbiologists-Delhi Chapter, 3^rd Annual Conference. AIIMS, New Delhi, India, 26^th March, 2011.
3. B. K. Chourasia, P. S. Nemavarkar and K. Pasupathy.  Radio protective action of compounds using “Saccharomyces cerevisiae”. International Conference on Radiation Damage and its Modification, INMAS, New Delhi, India from 13^th to 15^th November, 2002. 
4. Chourasia B. K, S. Premlatha M M, Varshney A. K, Dhawan B and Chaudhry R. Cloning of 16S rRNA fragment of Leptospira australis to be used as positive template in standardization and application of PCR. International conference on leptospirosis: A diagnostic enigma and the 6^th Scientific Meeting of the Indian Leptospirosis Society, School of Life sciences; University of Hyderabad, India from 20^th to 22^nd June, 2006.
5. B. K. Chourasia, Chaudhry R, Malhotra P. Scanning of Mycoplasma pneumoniae P1 protein for immunodominant and adhesive epitope(s). XXXIV National Conference of Indian Association of Medical Microbiologists MICROCON-2010, Kolkata, India from 24^th to 28^th November, 2010.

CONFERENCE AND SYMPOSIUMS ATTENDED

1. CME on Stem Cell Therapy. AIIMS, New Delhi, India, 2005.
2. Indian Society of Ocular Pathology-1^st Annual Meet-2006. AIIMS, New Delhi, India, 2006. 
3. Symposium on Stem Cell Applications-Promises and Potentials, Clinical and Experimental Aspects. Department of Pediatric Surgery, AIIMS, New Delhi, India, 2008.
4. ICTC Sensitization Training Programme-Cum-Workshop conducted by Delhi State AIDS Control Society (Delhi Government), AIIMS, New Delhi, 2009.
5. CME on “Emerging Trends in Mycoplasma” and meeting of Association of Mycoplasmologists of India, Indira Gandhi Medical College, Shimla, Himachal Pradesh, India, 2009.
6. 5^th Indo-Australian Conference on Medical Biotechnology. National Institute of Immunology, New Delhi, India, 2008.
7. National Conference on Medical Biotechnology-Vision 2020. Advanced Centre for Biotechnology, Maharshi Dayanand University, Rohtak, India, 2010.
8. IX National Conference-Cum-CME-Workshop of Indian Association of Mycoplasmologists. Government Medical College Hospital, Chandigarh, India, 2010. 

PRIZE/ACADEMIC ACHIEVEMENTS

• First prize in Science Model Exhibition (Beeta pleated sheet, a secondary structure of proteins), Patna University, Patna.
• Merit scholarship (2002) during M. Sc. Biotechnology, Bundelkhand University, Jhansi.
• Institute Fellowship award for Six Months (1^st May, 2008 -31^st October, 2008) from All India Institute of Medical Sciences (AIIMS), New Delhi, India.  
• Senior Research Fellowship award (January 2009- July 2012) from Indian Council of Medical Research (ICMR), New Delhi, India.
• Council of Scientific and Industrial Research (CSIR) and Department of Biotechnology, Government of India (DBT) travel grant award to attend the 18^th International Congress of IOM, held at Chianciano, Italy, 2010.

PERSONAL DETAILS

Nationality                   Indian

Permanent Address       Flat 5, 3^rd Floor, House No. 30, Vijay Block, Laxmi Nagar, Delhi110092, India.

Marital Status               Married

PROFESSIONAL REFERENCES will be provided on request.

Date:  

Place:                                                                                         (Bishwanath Kumar Chourasia)