TY - JOUR
T1 - Cereal β-glucan immune modulating activity depends on the polymer fine structure
AU - Mikkelsen, Mette Skau
AU - Jespersen, Birthe P Møller
AU - Mehlsen, Anni
AU - Engelsen, Søren Balling
AU - Frøkiær, Hanne
PY - 2014/8
Y1 - 2014/8
N2 - Scope: The relationship between the immune modulatory properties of different mixed linkage β-glucans from plant and microbial origin and their physico-chemical properties is elusive. By the use of murine dendritic cells and multivariate data analysis, this study examines the importance of the physico-chemical characteristics of β-glucans for their capacity to modulate immune responses. Methods and results: A total of 23 different β-glucan samples characterised with respect to compositional, molecular, structural and rheological properties were investigated for their immune modulatory properties. Most of the β-glucans showed a decrease in the LPS induced IL-12 production and an increase in the LPS induced IL-10 levels in dendritic cells. When the dendritic cells were stimulated with the probiotic Lactobacillus acidophilus NCFM, the β-glucans up-regulate the IL-12 production but showed no effect on the IL-10 levels. The modulation of the cytokine responses was shown to depend on the β-glucan block structure as expressed by the ratio of cellotriosyl to cellotetraosyl units in the molecule as well as the polymer solubility and aggregation in solution. Conclusion: This comparative study reveals that the β-glucan fine structure and in turn the intermolecular organisation in solution are key factors that determine their immune modulatory capacity.
AB - Scope: The relationship between the immune modulatory properties of different mixed linkage β-glucans from plant and microbial origin and their physico-chemical properties is elusive. By the use of murine dendritic cells and multivariate data analysis, this study examines the importance of the physico-chemical characteristics of β-glucans for their capacity to modulate immune responses. Methods and results: A total of 23 different β-glucan samples characterised with respect to compositional, molecular, structural and rheological properties were investigated for their immune modulatory properties. Most of the β-glucans showed a decrease in the LPS induced IL-12 production and an increase in the LPS induced IL-10 levels in dendritic cells. When the dendritic cells were stimulated with the probiotic Lactobacillus acidophilus NCFM, the β-glucans up-regulate the IL-12 production but showed no effect on the IL-10 levels. The modulation of the cytokine responses was shown to depend on the β-glucan block structure as expressed by the ratio of cellotriosyl to cellotetraosyl units in the molecule as well as the polymer solubility and aggregation in solution. Conclusion: This comparative study reveals that the β-glucan fine structure and in turn the intermolecular organisation in solution are key factors that determine their immune modulatory capacity.
U2 - 10.1016/j.foodres.2014.04.021
DO - 10.1016/j.foodres.2014.04.021
M3 - Journal article
SN - 0963-9969
VL - 62
SP - 829
EP - 836
JO - Food Research International
JF - Food Research International
ER -