In-chip fabrication of free-form 3D constructs for directed cell migration analysis

Mark Holm Olsen; Gertrud Malene Hjortø; Morten Hansen; Özcan Met; Inge Marie Svane; Niels B. Larsen

doi:10.1039/c3lc50930c

In-chip fabrication of free-form 3D constructs for directed cell migration analysis

Mark Holm Olsen, Gertrud Malene Hjortø, Morten Hansen, Özcan Met, Inge Marie Svane, Niels B. Larsen^*

^*Corresponding author for this work

Department of Political Science

41 Citations (Scopus)

Abstract

Free-form constructs with three-dimensional (3D) microporosity were fabricated by two-photon polymerization inside the closed microchannel of an injection-molded, commercially available polymer chip for analysis of directed cell migration. Acrylate constructs were produced as woodpile topologies with a range of pore sizes from 5 × 5 μm to 15 × 15 μm and prefilled with fibrillar collagen. Dendritic cells seeded into the polymer chip in a concentration gradient of the chemoattractant CCL21 efficiently negotiated the microporous maze structure for pore sizes of 8 × 8 μm or larger. The cells migrating through smaller pore sizes made significantly more turns than those through larger pores. The introduction of additional defined barriers in the microporous structure resulted in dendritic cells making more turns while still being able to follow the chemoattractant concentration gradient.

Original language	English
Journal	Lab On a Chip
Volume	13
Issue number	24
Pages (from-to)	4800-4809
Number of pages	10
ISSN	1473-0197
DOIs	https://doi.org/10.1039/c3lc50930c
Publication status	Published - 21 Dec 2013

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AU - Olsen, Mark Holm

AU - Hjortø, Gertrud Malene

AU - Hansen, Morten

AU - Met, Özcan

AU - Svane, Inge Marie

AU - Larsen, Niels B.

PY - 2013/12/21

Y1 - 2013/12/21

N2 - Free-form constructs with three-dimensional (3D) microporosity were fabricated by two-photon polymerization inside the closed microchannel of an injection-molded, commercially available polymer chip for analysis of directed cell migration. Acrylate constructs were produced as woodpile topologies with a range of pore sizes from 5 × 5 μm to 15 × 15 μm and prefilled with fibrillar collagen. Dendritic cells seeded into the polymer chip in a concentration gradient of the chemoattractant CCL21 efficiently negotiated the microporous maze structure for pore sizes of 8 × 8 μm or larger. The cells migrating through smaller pore sizes made significantly more turns than those through larger pores. The introduction of additional defined barriers in the microporous structure resulted in dendritic cells making more turns while still being able to follow the chemoattractant concentration gradient.

AB - Free-form constructs with three-dimensional (3D) microporosity were fabricated by two-photon polymerization inside the closed microchannel of an injection-molded, commercially available polymer chip for analysis of directed cell migration. Acrylate constructs were produced as woodpile topologies with a range of pore sizes from 5 × 5 μm to 15 × 15 μm and prefilled with fibrillar collagen. Dendritic cells seeded into the polymer chip in a concentration gradient of the chemoattractant CCL21 efficiently negotiated the microporous maze structure for pore sizes of 8 × 8 μm or larger. The cells migrating through smaller pore sizes made significantly more turns than those through larger pores. The introduction of additional defined barriers in the microporous structure resulted in dendritic cells making more turns while still being able to follow the chemoattractant concentration gradient.

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In-chip fabrication of free-form 3D constructs for directed cell migration analysis

Abstract

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