In-chip fabrication of free-form 3D constructs for directed cell migration analysis

TY - JOUR

T1 - In-chip fabrication of free-form 3D constructs for directed cell migration analysis

AU - Olsen, Mark Holm

AU - Hjortø, Gertrud Malene

AU - Hansen, Morten

AU - Met, Özcan

AU - Svane, Inge Marie

AU - Larsen, Niels B.

PY - 2013/12/21

Y1 - 2013/12/21

N2 - Free-form constructs with three-dimensional (3D) microporosity were fabricated by two-photon polymerization inside the closed microchannel of an injection-molded, commercially available polymer chip for analysis of directed cell migration. Acrylate constructs were produced as woodpile topologies with a range of pore sizes from 5 × 5 μm to 15 × 15 μm and prefilled with fibrillar collagen. Dendritic cells seeded into the polymer chip in a concentration gradient of the chemoattractant CCL21 efficiently negotiated the microporous maze structure for pore sizes of 8 × 8 μm or larger. The cells migrating through smaller pore sizes made significantly more turns than those through larger pores. The introduction of additional defined barriers in the microporous structure resulted in dendritic cells making more turns while still being able to follow the chemoattractant concentration gradient.

AB - Free-form constructs with three-dimensional (3D) microporosity were fabricated by two-photon polymerization inside the closed microchannel of an injection-molded, commercially available polymer chip for analysis of directed cell migration. Acrylate constructs were produced as woodpile topologies with a range of pore sizes from 5 × 5 μm to 15 × 15 μm and prefilled with fibrillar collagen. Dendritic cells seeded into the polymer chip in a concentration gradient of the chemoattractant CCL21 efficiently negotiated the microporous maze structure for pore sizes of 8 × 8 μm or larger. The cells migrating through smaller pore sizes made significantly more turns than those through larger pores. The introduction of additional defined barriers in the microporous structure resulted in dendritic cells making more turns while still being able to follow the chemoattractant concentration gradient.

UR - http://www.scopus.com/inward/record.url?scp=84887516677&partnerID=8YFLogxK

U2 - 10.1039/c3lc50930c

DO - 10.1039/c3lc50930c

M3 - Journal article

C2 - 24153393

AN - SCOPUS:84887516677

SN - 1473-0197

VL - 13

SP - 4800

EP - 4809

JO - Lab On a Chip

JF - Lab On a Chip

IS - 24

ER -