@inbook{33174340008b4a4d991e09c807d5d930,
title = "Immune monitoring using mRNA-transfected dendritic cells",
abstract = "Dendritic cells are known to be the most potent antigen presenting cell in the immune system and are used as cellular adjuvants in therapeutic anticancer vaccines using various tumor-associated antigens or their derivatives. One way of loading antigen into the dendritic cells is by mRNA electroporation, ensuring presentation of antigen through major histocompatibility complex I and potentially activating T cells, enabling them to kill the tumor cells. Despite extensive research in the field, only one dendritic cell-based vaccine has been approved. There is therefore a great need to elucidate and understand the immunological impact of dendritic cell vaccination in order to improve clinical benefit. In this chapter, we describe a method for performing immune monitoring using peripheral blood mononuclear cells and autologous dendritic cells transfected with tumor-associated antigen-encoding mRNA.",
keywords = "Cancer immunotherapy, Dendritic cell, Electroporation, Immune monitoring, mRNA transfection, Vaccination",
author = "Borch, {Troels Holz} and Svane, {Inge Marie} and {\"O}zcan Met",
year = "2016",
doi = "10.1007/978-1-4939-3625-0_16",
language = "English",
isbn = "978-1-4939-3623-6",
volume = "1428",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "245--259",
booktitle = "Synthetic mRNA",
address = "United States",
}
