Abstract
Dendritic cells are known to be the most potent antigen presenting cell in the immune system and are used as cellular adjuvants in therapeutic anticancer vaccines using various tumor-associated antigens or their derivatives. One way of loading antigen into the dendritic cells is by mRNA electroporation, ensuring presentation of antigen through major histocompatibility complex I and potentially activating T cells, enabling them to kill the tumor cells. Despite extensive research in the field, only one dendritic cell-based vaccine has been approved. There is therefore a great need to elucidate and understand the immunological impact of dendritic cell vaccination in order to improve clinical benefit. In this chapter, we describe a method for performing immune monitoring using peripheral blood mononuclear cells and autologous dendritic cells transfected with tumor-associated antigen-encoding mRNA.
| Originalsprog | Engelsk |
|---|---|
| Titel | Synthetic mRNA : Production, Introduction Into Cells, and Physiological Consequences |
| Antal sider | 15 |
| Vol/bind | 1428 |
| Forlag | Humana Press |
| Publikationsdato | 2016 |
| Sider | 245-259 |
| ISBN (Trykt) | 978-1-4939-3623-6 |
| ISBN (Elektronisk) | 978-1-4939-3625-0 |
| DOI | |
| Status | Udgivet - 2016 |
| Navn | Methods in Molecular Biology |
|---|---|
| Vol/bind | 1428 |
| ISSN | 1064-3745 |
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