In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines

Martin Roursgaard; Kristina Bram Knudsen; Helle Northeved; Mikael Persson; Tina Christensen; Pramod E. K. Kumar; Anders Permin; Thomas L. Andresen; Torben Gjetting; Jens Lykkesfeldt; Lise K. Vesterdal; Steffen Loft; Peter Møller

doi:10.1016/j.tiv.2016.08.002

In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines

Martin Roursgaard, Kristina Bram Knudsen, Helle Northeved, Mikael Persson, Tina Christensen, Pramod E. K. Kumar, Anders Permin, Thomas L. Andresen, Torben Gjetting, Jens Lykkesfeldt, Lise K. Vesterdal, Steffen Loft, Peter Møller

29 Citations (Scopus)

Abstract

The aim of this study was to compare the effects of cationic micelle and liposome drug delivery systems on liver and lung cells in a toxicological in vitro screening model, with observations on cytotoxicity and genotoxicity. A screening battery was established for assessment of a broad range of parameters related to adverse effects. Clear concentration response effects were observed related to impairment of mitochondrial function, membrane integrity and oxidative stress markers, but no effect was observed on genotoxicity. The adverse effects were highest for the liposomes. The High Content Screening seems optimal for initial screening of adverse effects, and combined with standard cytotoxicity measurements initial screening can be performed for predictive toxicological screening.

Original language	English
Journal	Toxicology in Vitro
Volume	36
Pages (from-to)	164-171
Number of pages	8
ISSN	0887-2333
DOIs	https://doi.org/10.1016/j.tiv.2016.08.002
Publication status	Published - 1 Oct 2016

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Roursgaard, M., Knudsen, K. B., Northeved, H., Persson, M., Christensen, T., Kumar, P. E. K., Permin, A., Andresen, T. L., Gjetting, T., Lykkesfeldt, J., Vesterdal, L. K., Loft, S., & Møller, P. (2016). In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines. Toxicology in Vitro, 36, 164-171. https://doi.org/10.1016/j.tiv.2016.08.002

Roursgaard, M, Knudsen, KB, Northeved, H, Persson, M, Christensen, T, Kumar, PEK, Permin, A, Andresen, TL, Gjetting, T, Lykkesfeldt, J, Vesterdal, LK, Loft, S & Møller, P 2016, 'In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines', Toxicology in Vitro, vol. 36, pp. 164-171. https://doi.org/10.1016/j.tiv.2016.08.002

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AU - Christensen, Tina

AU - Kumar, Pramod E. K.

AU - Permin, Anders

AU - Andresen, Thomas L.

AU - Gjetting, Torben

AU - Lykkesfeldt, Jens

AU - Vesterdal, Lise K.

AU - Loft, Steffen

AU - Møller, Peter

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AB - The aim of this study was to compare the effects of cationic micelle and liposome drug delivery systems on liver and lung cells in a toxicological in vitro screening model, with observations on cytotoxicity and genotoxicity. A screening battery was established for assessment of a broad range of parameters related to adverse effects. Clear concentration response effects were observed related to impairment of mitochondrial function, membrane integrity and oxidative stress markers, but no effect was observed on genotoxicity. The adverse effects were highest for the liposomes. The High Content Screening seems optimal for initial screening of adverse effects, and combined with standard cytotoxicity measurements initial screening can be performed for predictive toxicological screening.

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ER -

In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines

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