Zhang Yang

Research output

Research output per year 2009 2014 2018 2019 2019

• 23 Journal article
• 1 Ph.D. thesis
• 1 Book chapter
• 1 Conference abstract in journal

Research output per year

Research output per year

Search results

• 2019

  Activity of N-acylneuraminate-9-phosphatase (NANP) is not essential for de novo sialic acid biosynthesis

  Willems, A. P., Sun, L., Schulz, M. A., Tian, W., Ashikov, A., van Scherpenzeel, M., Hermans, E., Clausen, H., Yang, Z. & Lefeber, D. J., 2019, In: Biochimica et Biophysica Acta - General Subjects. 1863, 10, p. 1471-1479 9 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • N-acylneuraminate-9-phosphatase 100%
  • N-Acetylneuraminic Acid 46%
  • Sialic Acid 44%
  • Biosynthesis 33%
  • Cytidine Monophosphate N-Acetylneuraminic Acid 19%
  5 Citations (Scopus)

• An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells

  Narimatsu, Y., Joshi, H. J., Nason, R., Van Coillie, J., Karlsson, R., Sun, L., Ye, Z., Chen, Y-H., Schjoldager, K. T., Steentoft, C., Furukawa, S., Bensing, B. A., Sullam, P. M., Thompson, A. J., Paulson, J. C., Büll, C., Adema, G. J., Mandel, U., Hansen, L., Bennett, E. P., & 4 othersVarki, A., Vakhrushev, S. Y., Yang, Z. & Clausen, H., 25 Jul 2019, In: Molecular Cell. 75, 2, p. 394-407, e1-e5

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Atlases 100%
  • Glycosylation 95%
  • Polysaccharides 87%
  • Genes 37%
  • Glycosyltransferases 12%
  50 Citations (Scopus)

• A validated collection of mouse monoclonal antibodies to human glycosyltransferases functioning in mucin-type O-glycosylation.

  Steentoft, C., Yang, Z., Wang, S., Ju, T., Vester-Christensen, M. B. & Hansen, L., 1 Sept 2019, In: Glycobiology. 29, p. 645–656 11 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  3 Citations (Scopus)

• Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA

  König, S., Yang, Z., Wandall, H. H., Mussolino, C. & Bennett, E. P., 2019, CRISPR Gene Editing. Luo, Y. (ed.). Humana Press, p. 45-66 22 p. (Methods in Molecular Biology, Vol. 1961).

  Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

  • Guide RNA 100%
  • Clustered Regularly Interspaced Short Palindromic Repeats 62%
  • Genome 49%
  • Frameshift Mutation 22%
  • Gene Knockout Techniques 21%
  1 Citation (Scopus)

• Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts

  Petersen, B. L., Möller, S. R., Mravec, J., Jørgensen, B., Christensen, M., Liu, Y., Wandall, H. H., Bennett, E. P. & Yang, Z., 17 Jun 2019, In: BMC Biotechnology. 19, p. 1-12 36.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Protoplasts 100%
  • Gene Editing 86%
  • Clustered Regularly Interspaced Short Palindromic Repeats 81%
  • Fluorescence 57%
  • Flow Cytometry 54%
  3 Citations (Scopus)

  49 Downloads (Pure)

• Targeted analysis of lysosomal directed proteins and their sites of mannose-6-phosphate modification

  Caval, T., Zhu, J., Tian, W., Remmelzwaal, S., Yang, Z., Clausen, H. & Heck, A. J. R., 2019, In: Molecular and Cellular Proteomics. 18, 1, p. 16-27 12 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Mannose 6-Phosphate 100%
  • mannose-6-phosphate 98%
  • lysosomal proteins 95%
  • Modification 36%
  • Glycoprotein 35%
  17 Citations (Scopus)

• The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells

  Tian, W., Ye, Z., Wang, S., Schulz, M. A., Van Coillie, J., Sun, L., Chen, Y-H., Narimatsu, Y., Hansen, L., Kristensen, C., Mandel, U., Bennett, E. P., Jabbarzadeh-Tabrizi, S., Schiffmann, R., Shen, J-S., Vakhrushev, S. Y., Clausen, H. & Yang, Z., 1 Dec 2019, In: Nature Communications. 10, 13 p., 1785.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • CHO Cells 100%
  • enzymes 88%
  • Glycosylation 85%
  • Polysaccharides 65%
  • Enzymes 45%
  21 Citations (Scopus)

  10 Downloads (Pure)
• 2018

  Direct quality control of glycoengineered erythropoietin variants

  Čaval, T., Tian, W., Yang, Z., Clausen, H. & Heck, A. J. R., 2018, In: Nature Communications. 9, 8 p., 3342.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Erythropoietin 100%
  • quality control 78%
  • Glycosylation 72%
  • Quality Control 68%
  • profiles 37%
  27 Citations (Scopus)

  49 Downloads (Pure)

• EDEM1’s mannosidase-like domain binds ERAD client proteins in a redox-sensitive manner and possesses catalytic activity

  Lamriben, L., Oster, M. E., Tamura, T., Tian, W., Yang, Z., Clausen, H. & Hebert, D. N., 7 Sept 2018, In: Journal of Biological Chemistry. 293, 36, p. 13932-13945 14 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Mannosidases 100%
  • Oxidation-Reduction 57%
  • Proteolysis 56%
  • Protein 24%
  • Proteins 22%
  15 Citations (Scopus)

• Galectin binding to cells and glycoproteins with genetically modified glycosylation reveals galectin–glycan specificities in a natural context

  Nielsen, M. I., Stegmayr, J., Grant, O. C., Yang, Z., Nilsson, U. J., Boos, I., Carlsson, M. C., Woods, R. J., Unverzagt, C., Leffler, H. & Wandall, H. H., 28 Dec 2018, In: Journal of Biological Chemistry. 293, 52, p. 20249-20262 14 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Galectins 100%
  • Glycosylation 74%
  • Polysaccharides 67%
  • Glycoprotein 66%
  • Glycoproteins 61%
  19 Citations (Scopus)

• Glycoengineering design options for IgG1 in CHO cells using precise gene editing

  Schulz, M. A., Tian, W., Mao, Y., Van Coillie, J., Sun, L., Larsen, J. S., Chen, Y-H., Kristensen, C., Vakhrushev, S. Y., Clausen, H. & Yang, Z., 1 Jul 2018, In: Glycobiology. 28, 7, p. 542–549

  Research output: Contribution to journal › Journal article › Research › peer-review

  • N-Glycan 100%
  • Gene Editing 96%
  • CHO Cells 94%
  • Polysaccharides 73%
  • Immunoglobulin G 59%
  16 Citations (Scopus)

• The GAGOme: a cell-based library of displayed glycosaminoglycans

  Chen, Y-H., Narimatsu, Y., Clausen, T. M., Gomes, C., Karlsson, R., Steentoft, C., Spliid, C. B., Gustavsson, T., Salanti, A., Persson, A., Malmström, A., Willén, D., Ellervik, U., Bennett, E. P., Mao, Y., Clausen, H. & Yang, Z., 1 Nov 2018, In: Nature Methods. 15, 11, p. 881-888

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Glycosaminoglycan 100%
  • Glycosaminoglycans 91%
  • Libraries 76%
  • Polysaccharides 16%
  • Gene Knock-In Techniques 14%
  36 Citations (Scopus)
• 2017

  Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis

  Lonowski, L. A., Narimatsu, Y., Riaz, A., Delay, C. M. E., Yang, Z., Niola, F., Duda, K., Ober, E. A., Clausen, H., Wandall, H. H., Hansen, S. H., Bennett, E. P. & Frödin, M., 1 Mar 2017, In: Nature Protocols. 12, 3, p. 581-603 23 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Gene Editing 100%
  • Pipeline 94%
  • Clustered Regularly Interspaced Short Palindromic Repeats 62%
  • Cell Line 48%
  • Effector 44%
  54 Citations (Scopus)

• Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD

  Möller, S. R., Yi, X., Vel?squez, S. M., Gille, S., Hansen, P., Poulsen, C. P., Olsen, C. E., Rejzek, M., Parsons, H. T., Yang, Z., Wandall, H. H., Clausen, H., Field, R. A., Pauly, M., Estevez, J. M., Harholt, J., Ulvskov, P. & Petersen, B. L., 30 Mar 2017, In: Scientific Reports. 7, 16 p., 45341.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Plant Cells 100%
  • Charophyceae 77%
  • Transferases 75%
  • Cell Wall 74%
  • Viridiplantae 65%
  20 Citations (Scopus)

  116 Downloads (Pure)

• Mammalian O-mannosylation of Cadherins and Plexins is Independent of Protein O-mannosyltransferase 1 and 2

  Larsen, I. S. B., Narimatsu, Y., Joshi, H. J., Yang, Z., Harrison, O. J., Brasch, J., Shapiro, L., Honig, B., Vakhrushev, S. Y., Clausen, H. & Halim, A., 7 Jul 2017, In: The Journal of Biological Chemistry. 292, p. 11586-11598

  Research output: Contribution to journal › Journal article › Research › peer-review

  • protein O-mannosyltransferase 100%
  • plexin 83%
  • Mannose 61%
  • Cadherins 53%
  • Dystroglycans 34%
  20 Citations (Scopus)
• 2015

  Engineered CHO cells for production of diverse, homogeneous glycoproteins

  Yang, Z., Wang, S., Halim, A., Schulz, M. A., Frödin, M., Rahman, S. H., Vester-Christensen, M. B., Behrens, C., Kristensen, C., Vakhrushev, S. Y., Bennett, E. P., Wandall, H. H. & Clausen, H., 10 Aug 2015, In: Nature Biotechnology. 33, 8, p. 842-844 3 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Glycosylation 100%
  • Glycoproteins 97%
  • Cricetulus 60%
  • Glycoprotein 52%
  • Ovary 49%
  129 Citations (Scopus)

• Fast and sensitive detection of indels induced by precise gene targeting

  Yang, Z., Steentoft, C., Hauge, C., Hansen, L., Thomsen, A. L., Niola, F., Vester-Christensen, M. B., Frödin, M., Clausen, H., Wandall, H. H. & Bennett, E. P., 19 May 2015, In: Nucleic Acids Research. 43, 9, p. 1-8 8 p., e59.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Gene Targeting 100%
  • Gene Editing 78%
  • Capillary Electrophoresis 27%
  • Double-Stranded DNA Breaks 24%
  • DNA 23%
  83 Citations (Scopus)

• Shaping Glycans by DNA Scissors: Turning Glycosciences Into Lego Toying

  Clausen, H., Schjoldager, K., Yang, Z., Steentoft, C., Halim, A., Bennett, E. & Vakhrushev, S., Nov 2015, In: Glycobiology. 25, 11, p. 1233-1233 1 p.

  Research output: Contribution to journal › Conference abstract in journal › Research › peer-review
• 2014

  Characterization of Binding Epitopes of CA125 Monoclonal Antibodies

  da Silva, L. P. M., Narimatsu, Y., Halim, A., Vieira Campos, D. A., Yang, Z., Tarp, M. P. A., Pereira, P. J. B., Mandel, U., Bennett, E. P., Vakhrushev, S. Y., Levery, S. B., David, L. & Clausen, H., 3 Jul 2014, In: Journal of Proteome Research. 13, 7, p. 3349-59 11 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Glycosylation 100%
  • Epitope 99%
  • Tandem Repeat Sequences 99%
  • Epitopes 83%
  • Monoclonal Antibodies 71%
  25 Citations (Scopus)

• High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs

  Duda, K., Lonowski, L. A., Kofoed-Nielsen, M., Ibarra, A., Delay, C. M., Kang, Q., Yang, Z., Pruett-Miller, S. M., Bennett, E. P., Wandall, H. H., Davis, G. D., Hansen, S. H. & Frödin, M., 2 Jun 2014, In: Nucleic Acids Research. 42, 10, p. 1-16 16 p., e84.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Zinc Finger Nucleases 100%
  • Gene Editing 79%
  • Deoxyribonuclease I 77%
  • Clustered Regularly Interspaced Short Palindromic Repeats 74%
  • Mutagenesis 63%
  56 Citations (Scopus)

• Low Density Lipoprotein Receptor Class A Repeats Are O-Glycosylated in Linker Regions

  Pedersen, N. B., Wang, S., Narimatsu, Y., Yang, Z., Halim, A., Schjoldager, K. T-B. G., Madsen, T. D., Seidah, N. G., Bennett, E. P., Levery, S. B. & Clausen, H., 20 Jun 2014, In: The Journal of Biological Chemistry. 289, 25, p. 17312-17324 13 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Low-Density Lipoprotein 100%
  • LDL Receptors 99%
  • Glycosylation 58%
  • CHO Cells 17%
  • polypeptide N-acetylgalactosaminyltransferase 11%
  29 Citations (Scopus)

• The GalNAc-type O-glycoproteome of CHO cells characterized by the SimpleCell strategy

  Yang, Z., Halim, A., Narimatsu, Y., Joshi, H. J., Steentoft, C., Schjoldager, K. T-B. G., Schulz, M. A., Sealover, N. R., Kayser, K. J., Bennett, E. P., Levery, S. B., Vakhrushev, S. Y. & Clausen, H., 1 Dec 2014, In: Molecular & Cellular Proteomics. 13, 12, p. 3224-3235 12 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • O-Glycoprotein 100%
  • Cricetulus 59%
  • Ovary 48%
  • O-Glycan 40%
  • Glycosylation 27%
  53 Citations (Scopus)
• 2012

  Engineering Mammalian Mucin-type O-Glycosylation in Plants

  Yang, Z., Drew, D. P., Jørgensen, B., Mandel, U., Bach, S. S., Ulvskov, P., Levery, S. B., Bennett, E. P., Clausen, H. & Petersen, B. L., 6 Apr 2012, In: Journal of Biological Chemistry. 287, 15, p. 11911-23 13 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • O-Glycoprotein 100%
  • Mucins 89%
  • Glycosylation 85%
  • Hydroxyproline 73%
  • polypeptide N-acetylgalactosaminyltransferase 62%
  46 Citations (Scopus)

• Trans-species Engineering of Glycosylated Therapeutic Proteins

  Yang, Z., 15 May 2012

  Research output: Book/Report › Ph.D. thesis
• 2010

  A combined zinc/cadmium sensor and zinc/cadmium export regulator in a heavy metal pump

  Bækgaard, L., Mikkelsen, M. D., Sørensen, D. M., Hegelund, J. N., Persson, D. O., Mills, R. F., Yang, Z., Husted, S., Andersen, J. P., Buch-Pedersen, M. J., Schjørring, J. K., Williams, L. E. & Palmgren, M. B., 8 Oct 2010, In: Journal of Biological Chemistry. 285, 41, p. 31243-31252 10 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  • Heavy Metals 100%
  • Cadmium 95%
  • Pump 81%
  • Zinc 80%
  • Heavy Metal 70%
  51 Citations (Scopus)
• 2009

  Type f Haemophilus influenzae cellulitis in the upper extremities of an immunocompetent adult

  Zhang, Y. & Engelhart, M., 2009, In: Clinical and Experimental Rheumatology. 27, 1, p. 163 1 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  3 Citations (Scopus)