Engineered CHO cells for production of diverse, homogeneous glycoproteins

Zhang Yang; Shengjun Wang; Adnan Halim; Morten Alder Schulz; Morten Frödin; Shamim H Rahman; Malene Bech Vester-Christensen; Carsten Behrens; Claus Kristensen; Sergey Y Vakhrushev; Eric Paul Bennett; Hans H Wandall; Henrik Clausen

doi:10.1038/nbt.3280

Engineered CHO cells for production of diverse, homogeneous glycoproteins

Zhang Yang, Shengjun Wang, Adnan Halim, Morten Alder Schulz, Morten Frödin, Shamim H Rahman, Malene Bech Vester-Christensen, Carsten Behrens, Claus Kristensen, Sergey Y Vakhrushev, Eric Paul Bennett, Hans H Wandall, Henrik Clausen

129 Citations (Scopus)

Abstract

Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.

Original language	English
Journal	Nature Biotechnology
Volume	33
Issue number	8
Pages (from-to)	842-844
Number of pages	3
ISSN	1087-0156
DOIs	https://doi.org/10.1038/nbt.3280
Publication status	Published - 10 Aug 2015

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10.1038/nbt.3280

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title = "Engineered CHO cells for production of diverse, homogeneous glycoproteins",

abstract = "Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.",

author = "Zhang Yang and Shengjun Wang and Adnan Halim and Schulz, {Morten Alder} and Morten Fr{\"o}din and Rahman, {Shamim H} and Vester-Christensen, {Malene Bech} and Carsten Behrens and Claus Kristensen and Vakhrushev, {Sergey Y} and Bennett, {Eric Paul} and Wandall, {Hans H} and Henrik Clausen",

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doi = "10.1038/nbt.3280",

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T1 - Engineered CHO cells for production of diverse, homogeneous glycoproteins

AU - Yang, Zhang

AU - Wang, Shengjun

AU - Halim, Adnan

AU - Schulz, Morten Alder

AU - Frödin, Morten

AU - Rahman, Shamim H

AU - Vester-Christensen, Malene Bech

AU - Behrens, Carsten

AU - Kristensen, Claus

AU - Vakhrushev, Sergey Y

AU - Bennett, Eric Paul

AU - Wandall, Hans H

AU - Clausen, Henrik

PY - 2015/8/10

Y1 - 2015/8/10

N2 - Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.

AB - Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.

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DO - 10.1038/nbt.3280

M3 - Journal article

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VL - 33

SP - 842

EP - 844

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 8

ER -

Engineered CHO cells for production of diverse, homogeneous glycoproteins

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