Jens-Christian Navarro Poulsen

2019

Insights into an unusual Auxiliary Activity 9 family member lacking the histidine brace motif of lytic polysaccharide monooxygenases

Frandsen, K. E. H., Tovborg, M., Jørgensen, C. I., Spodsberg, N., Rosso, M-N., Hemsworth, G. R., Garman, E. F., Grime, G. W., Poulsen, J-C. N., Batth, T. S., Miyauchi, S., Lipzen, A., Daum, C., Grigoriev, I. V., Johansen, K. S., Henrissat, B., Berrin, J-G. & Lo Leggio, L., 8 Nov 2019, In: The Journal of Biological Chemistry. 294, p. 17117-17130 24 p.

Research output: Contribution to journal › Journal article › Research › peer-review

9 Citations (Scopus)

Structure and Dynamics of a Promiscuous Xanthan Lyase from Paenibacillus nanensis and the Design of Variants with Increased Stability and Activity

Jensen, P. F., Kadziola, A., Comamala, G., Segura, D. R., Anderson, L., Poulsen, J-C. N., Rasmussen, K. K., Agarwal, S., Sainathan, R. K., Monrad, R. N., Svendsen, A., Nielsen, J. E., Lo Leggio, L. & Rand, K. D., 21 Feb 2019, In: Cell Chemical Biology. 26, 2, p. 191-202.e1-e6 19 p.

Research output: Contribution to journal › Journal article › Research › peer-review

3 Citations (Scopus)

Structure–function analyses reveal that a glucuronoyl esterase from Teredinibacter turnerae interacts with carbohydrates and aromatic compounds

Arnling Bååth, J., Mazurkewich, S., Poulsen, J. N., Olsson, L., Lo Leggio, L. & Larsbrink, J., 19 Apr 2019, In: Journal of Biological Chemistry. 294, 16, p. 6635-6644 10 p.

Research output: Contribution to journal › Journal article › Research › peer-review

5 Citations (Scopus)

9 Downloads (Pure)

Structure of Aspergillus aculeatus β-1,4-galactanase in complex with galactobiose

Torpenholt, S., Poulsen, J. N., Muderspach, S. J., De Maria, L. & Lo Leggio, L., Jun 2019, In: Acta Crystallographica Section F: Structural Biology Communications. 75, 6, p. 399-404 6 p.

Research output: Contribution to journal › Journal article › Research › peer-review

1 Citation (Scopus)

14 Downloads (Pure)

2018

Biochemical and structural features of diverse bacterial glucuronoyl esterases facilitating recalcitrant biomass conversion

Baath, J. A., Mazurkewich, S., Knudsen, R. M., Poulsen, J-C. N., Olsson, L., Lo Leggio, L. & Larsbrink, J., 1 Aug 2018, In: Biotechnology for Biofuels. 11, 14 p., 213.

Research output: Contribution to journal › Journal article › Research › peer-review

13 Citations (Scopus)

65 Downloads (Pure)

Structure of a hyperthermostable carbonic anhydrase identified from an active hydrothermal vent chimney

Fredslund, F., Borchert, M. S., Poulsen, J-C. N., Mortensen, S. B., Perner, M., Streit, W. R. & Lo Leggio, L., Jul 2018, In: Enzyme and Microbial Technology. 114, p. 48-54 7 p.

Research output: Contribution to journal › Journal article › Research › peer-review

4 Citations (Scopus)

Structure of a lytic polysaccharide monooxygenase from Aspergillus fumigatus and an engineered thermostable variant

Lo Leggio, L., Weihe, C. D., Poulsen, J. N., Sweeney, M., Rasmussen, F., Lin, J., De Maria, L. & Wogulis, M., Nov 2018, In: Carbohydrate Research. 469, p. 55-59 5 p.

Research output: Contribution to journal › Journal article › Research › peer-review

16 Citations (Scopus)

2017

Determination of protonation states of iminosugar-enzyme complexes using photoinduced electron transfer

Wang, B., Olsen, J. I., Laursen, B. W., Poulsen, J. C. N. & Bols, M., 2017, In: Chemical Science. 8, 11, p. 7383-7393 11 p.

Research output: Contribution to journal › Journal article › Research › peer-review

7 Citations (Scopus)

78 Downloads (Pure)

Learning from oligosaccharide soaks of crystals of an AA13 lytic polysaccharide monooxygenase: crystal packing, ligand binding and active-site disorder

Frandsen, K. E. H., Poulsen, J-C. N., Tovborg, M., Johansen, K. S. & Lo Leggio, L., 1 Jan 2017, In: Acta crystallographica Section D: Structural biology . 73, p. 64-76 13 p.

Research output: Contribution to journal › Journal article › Research › peer-review

10 Citations (Scopus)

118 Downloads (Pure)

Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates

Simmons, T. J., Frandsen, K. E. H., Ciano, L., Tryfona, T., Lenfant, N., Poulsen, J. C., Wilson, L. F. L., Tandrup, T., Tovborg, M., Schnorr, K., Johansen, K. S., Henrissat, B., Walton, P. H., Lo Leggio, L. & Dupree, P., 1 Dec 2017, In: Nature Communications. 8, 12 p., 1064.

Research output: Contribution to journal › Journal article › Research › peer-review

69 Citations (Scopus)

82 Downloads (Pure)

Unliganded and substrate bound structures of the cellooligosaccharide active lytic polysaccharide monooxygenase LsAA9A at low pH

Frandsen, K. E. H., Poulsen, J-C. N., Tandrup, T. & Lo Leggio, L., 2017, In: Carbohydrate Research. 448, p. 187-190 4 p.

Research output: Contribution to journal › Journal article › Research › peer-review

15 Citations (Scopus)

2016

Structural characterization of the thermostable Bradyrhizobium japonicum D-sorbitol dehydrogenase

Fredslund, F., Otten, H., Gemperlein, S., Poulsen, J-C. N., Carius, Y., Kohring, G-W. & Lo Leggio, L., 1 Nov 2016, In: Acta Crystallographica Section F. 72, 11, p. 846-852 7 p.

Research output: Contribution to journal › Journal article › Research › peer-review

3 Citations (Scopus)

85 Downloads (Pure)

The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases

Frandsen, K. E. H., Simmons, T. J., Dupree, P., Poulsen, J-C. N., Hemsworth, G. R., Ciano, L., Johnston, E. M., Tovborg, M., Johansen, K. S., von Freiesleben, P., Marmuse, L., Fort, S., Cottaz, S., Driguez, H., Henrissat, B., Lenfant, N., Tuna, F., Baldansuren, A., Davies, G. J., Lo Leggio, L., & 1 othersWalton, P. H., 1 Apr 2016, In: Nature Chemical Biology. 12, 4, p. 298-303 6 p.

Research output: Contribution to journal › Journal article › Research › peer-review

160 Citations (Scopus)

2015

Adenine phosphoribosyltransferase from Sulfolobus solfataricus is an enzyme with unusual kinetic properties and a crystal structure that suggests it evolved from a 6-oxopurine phosphoribosyltransferase

Jensen, K. F., Hansen, M. R., Jensen, K. S., Christoffersen, S., Poulsen, J-C. N., Mølgaard, A. & Kadziola, A., 14 Apr 2015, In: Biochemistry. 54, 14, p. 2323-2334 12 p.

Research output: Contribution to journal › Journal article › Research › peer-review

6 Citations (Scopus)

A fluorescence study of isofagomine protonation in β-glucosidase

Lindbäck, E. A., Laursen, B. W., Poulsen, J-C. N., Kilså, K., Pedersen, C. M. & Bols, M., 21 Jun 2015, In: Organic & Biomolecular Chemistry. 13, 23, p. 6562-6566 5 p.

Research output: Contribution to journal › Journal article › Research › peer-review

7 Citations (Scopus)

Structural diversity of lytic polysaccharide monooxygenases

Lo Leggio, L., Frandsen, K. E. H., Poulsen, J-C. N., Tovborg, M., De Maria, L. & Johansen, K. S., 2015, Proceedings of "Mie Bioforum 2014 - Lignocellulose Degradation and Biorefinery". Sakka, K., Kimura, T., Tamaru, Y., Karita, S. & Sakka, M. (eds.). UNI Publishers Co., Ltd, p. 148-156 9 p.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase

Lo Leggio, L., Simmons, T. J., Poulsen, J-C. N., Frandsen, K. E. H., Hemsworth, G. R., Stringer, M. A., von Freiesleben, P., Tovborg, M., Johansen, K. S., De Maria, L., Harris, P. V., Soong, C-L., Dupree, P., Tryfona, T., Lenfant, N., Henrissat, B., Davies, G. J. & Walton, P. H., 22 Jan 2015, In: Nature Communications. 6, 9 p., 5961.

Research output: Contribution to journal › Journal article › Research › peer-review

167 Citations (Scopus)

131 Downloads (Pure)

2014

A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease: a crystallographic and computational study

Czene, A., Tóth, E., Németh, E., Otten, H., Poulsen, J-C. N., Christensen, H. E. M., Rulíšek, L., Nagata, K., Larsen, S. & Gyurcsik, B., 2014, In: Metallomics. 6, 11, p. 2090-2099 10 p.

Research output: Contribution to journal › Journal article › Research › peer-review

5 Citations (Scopus)

67 Downloads (Pure)

Purification, crystal structure determination and functional characterization of type III antifreeze proteins from the European eelpout Zoarces viviparus

Wilkens, C., Poulsen, J-C. N., Ramløv, H. & Lo Leggio, L., Aug 2014, In: Cryobiology. 69, 1, p. 163-168 6 p.

Research output: Contribution to journal › Journal article › Research › peer-review

12 Citations (Scopus)

1668 Downloads (Pure)

2013

Binding of the N-terminal domain of the lactococcal bacteriophage TP901-1 CI repressor to its target DNA: a crystallography, small angle scattering, and nuclear magnetic resonance study

Frandsen, K. E. H., Rasmussen, K. K., Jensen, M. R., Hammer, K., Pedersen, M., Poulsen, J-C. N., Arleth, L. & Lo Leggio, L., 1 Oct 2013, In: Biochemistry. 52, 39, p. 6892-6904 13 p.

Research output: Contribution to journal › Journal article › Research › peer-review

10 Citations (Scopus)

Camel and bovine chymosin: the relationship between their structures and cheese-making properties

Jensen, J. L., Mølgaard, A., Poulsen, J-C. N., Harboe, M. K., Simonsen, J. B., Lorentzen, A. M., Hjernø, K., van den Brink, J. M., Qvist, K. B. & Larsen, S., May 2013, In: Acta Crystallographica. Section D: Biological Crystallography. D69, p. 901-913 13 p.

Research output: Contribution to journal › Journal article › Research › peer-review

33 Citations (Scopus)

Crystallization and preliminary crystallographic analysis of an Escherichia coli-selected mutant of the nuclease domain of the metallonuclease colicin E7

Czene, A., Toth, E., Gyurcsik, B., Otten, H., Poulsen, J-C. N., Lo Leggio, L., Larsen, S., Christensen, H. E. M. & Nagata, K., May 2013, In: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online. 69, p. 551-554 4 p.

Research output: Contribution to journal › Journal article › Research › peer-review

2 Citations (Scopus)

2012

Enzymology and structure of the GH13_31 glucan 1,6-a-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM

Møller, M. S., Fredslund, F., Majumder, A., Nakai, H., Poulsen, J-C. N., Lo Leggio, L., Svensson, B. & Abou Hachem, M., Aug 2012, In: Journal of Bacteriology. 194, 16, p. 4249-4259 11 p.

Research output: Contribution to journal › Journal article › Research › peer-review

45 Citations (Scopus)

2011

Activity of three ß-1,4-galactanases on small chromogenic substrates

Torpenholt, S. K., Le Nours, J., Christensen, U., Jahn, M., Withers, S., Østergaard, P., Borchert, T. V., Poulsen, J-C. N. & Lo Leggio, L., Sept 2011, In: Carbohydrate Research. 346, p. 2028-2033

Research output: Contribution to journal › Journal article › Research › peer-review

13 Citations (Scopus)

From screen to structure with a harvestable microfluidic device

Stojanoff, V., Jakoncic, J., Oren, D. A., Nagarajan, V., Poulsen, J-C. N., Adams-Cioaba, M. A., Bergfors, T. & Sommer, M. O. A., Aug 2011, In: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online. 67, 8, p. 971-975

Research output: Contribution to journal › Journal article › Research › peer-review

18 Citations (Scopus)

Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components to cleave cellulose

Quinlan, R. J., Sweeney, M. D., Lo Leggio, L., Otten, H., Poulsen, J-C. N., Johansen, K. S., Krogh, K. B. R. M., Jørgensen, C. I., Tovborg, M., Anthonsen, A., Tryfona, T., Tryfona, T., Walter, C. P., Dupree, P., Xu, F., Davies, G. J. & Walton, P. H., 13 Sept 2011, In: Proceedings of the National Academy of Sciences of the United States of America. 108, p. 15079-15084

Research output: Contribution to journal › Journal article › Research › peer-review

561 Citations (Scopus)

Structure of a fungal dimeric a-type carbonic anhydrase

Cuesta Seijo, J. A., Borchert, M. S., Poulsen, J-C. N., Schnorr, K. M., Mortensen, S. B. & Lo Leggio, L., 2011, In: F E B S Letters. 585, p. 1042-1048

Research output: Contribution to journal › Journal article › Research › peer-review

29 Citations (Scopus)

2010

Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: Structure and function of a large, enigmatic family

Harris, P. V., Welner, D. H., McFarland, KC., Re, E., Poulsen, J-C. N., Brown, K., Salbo, R., Ding, H., Vlasenko, E., Marino, S., Xu, F., Cherry, J., Larsen, S. & Lo Leggio, L., 20 Apr 2010, In: Biochemistry. 49, p. 3305-3316

Research output: Contribution to journal › Journal article › Research › peer-review

517 Citations (Scopus)

2008

Crystal Structure of NCAM2 Ig1 (I4122 Cell Unit)

Kulahin, N., Rasmussen, K. K., Kristensen, O., Kastrup, J. S. J., Poulsen, J-C. N., Berezin, V., Bock, E. M., Walmod, P. S. & Gajhede, M., 2008

Research output: Non-textual form › Dataset › Research

1125 Downloads (Pure)

Crystal structure of the Ig1 domain of the neural cell adhesion molecule NCAM2 displays domain swapping.

Rasmussen, K. K., Kulahin, N., Kristensen, O., Poulsen, J-C. N., Sigurskjold, B. W., Kastrup, J. S., Berezin, V., Bock, E., Walmod, P. S. & Gajhede, M., 2008, In: Journal of Molecular Biology. 382, 5, p. 1113-20 7 p.

Research output: Contribution to journal › Journal article › Research › peer-review

7 Citations (Scopus)

Short strong hydrogen bonds in proteins: a case study of rhamnogalacturonan acetylesterase

Langkilde, A. E., Kristensen, S. M., Lo Leggio, L., Mølgaard, A., Jensen, J. H., Houk, A. R., Poulsen, J-C. N., Larsen, S. Y. & Kauppinen, S., 2008, In: Acta Crystallographica. Section D: Biological Crystallography. 64, p. 851-863

Research output: Contribution to journal › Journal article › Research › peer-review

31 Citations (Scopus)

Structure and function of enzymes in biomass degradation

Welner, D. H., Jensen, M. H., McFarland, K. C., Poulsen, J-C. N., Otten, H., Salbo, R., Christensen, U., Harris, P., Larsen, S., Borchert, T., Christensen, L. L. H., Friis, E. P. & Lo Leggio, L., 2008, Biotechnology of lignocellulose degradation and Biomass Utilization - Mie Bioforum 2008. p. 349-355

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research

2004

High-throughput physical characterization of protein solutions using microfluides

Sommer, M. O. A., Poulsen, J-C. N. & Larsen, S., 2004, In: Acta Crystallographica. A60, p. 206

Research output: Contribution to journal › Journal article › Research › peer-review

Rhamnogalacturonan lyase reveals a unique three-domain modular structure for polysaccharide lyase family 4

McDonough, M. A., Kadirvelraj, R., Harris, P. H., Poulsen, J-C. N. & Larsen, S., 2004, In: FEBS Letters. 565, p. 188-194

Research output: Contribution to journal › Journal article › Research › peer-review

36 Citations (Scopus)

2003

Impact of the physical and chemical environment on the molecular structure of Coprinus cinereus peroxidase

Houborg, K., Harris, P. H., Petersen, J. G. L., Rowland, P., Poulsen, J-C. N., Schneider, P., Vind, J. & Larsen, S., 2003, In: Acta Crystallographica. Section D: Biological Crystallography. D59, p. 989-996

Research output: Contribution to journal › Journal article › Research › peer-review

The Structure of a mutant enzyme of Coprinus cinereus peroxidase provides an understanding of its increased thermostability

Houborg, K., Harris, P. H., Poulsen, J-C. N., Schneider, P., Svendsen, A. & Larsen, S., 2003, In: Acta Crystallographica. Section D: Biological Crystallography. D59, p. 997-1003

Research output: Contribution to journal › Journal article › Research › peer-review

2002

A stepwise optimization of crystals of rhamnogalacturonan lyase from Aspergillus aculeatus

Kadirvelraj, R., Harris, P. H., Poulsen, J-C. N., Kauppinen, S. & Larsen, S., 2002, In: Acta Crystallographica D. 58, p. 1346-1349

Research output: Contribution to journal › Journal article › Research › peer-review

Substrate Binding Induces Domain Movements in Orotidine 5'-Monophosphate Decarboxylase

Harris, P. H., Poulsen, J-C. N., Jensen, K. F. & Larsen, S., 2002, In: Journal of Molecular Biology. 318, 4, p. 1019-1029

Research output: Contribution to journal › Journal article › Research › peer-review

20 Citations (Scopus)

2001

pH, conductivity and long-term stability in the Crystal Screen solutions

Bukrinski, J. T. & Poulsen, J-C. N., 2001, In: Journal of Applied Crystallography. 34, p. 533-534 2 p.

Research output: Contribution to journal › Journal article › Research

8 Citations (Scopus)

Selenomethionine substitution of orotidine-5-monophosphate decarboxylase causes a change in crystal contacts and space group

Poulsen, J-C. N., Harris, P., Jensen, K. F. & Larsen, S., 2001, In: Acta Crystallographica. Section D: Biological Crystallography. 57, 9, p. 1251-1259

Research output: Contribution to journal › Journal article › Research › peer-review

9 Citations (Scopus)

Selenomethionine substitution of orotidine-5'-monophosphate decarboxylase causes a change in crystal contacts and space group

Poulsen, J-C. N., Harris, P. H., Jensen, K. F. & Larsen, S., 2001, In: Acta Crystallographica. Section D: Biological Crystallography. 57, 9, p. 1251-1259

Research output: Contribution to journal › Journal article › Research › peer-review

9 Citations (Scopus)

Selenomethionine substitution of orotidine-5′-monophosphate decarboxylase causes a change in crystal contacts and space group

Poulsen, J-C. N., Harris, P. H., Jensen, K. F. & Larsen, S., 2001, In: Acta Crystallographica. Section D: Biological Crystallography. 57, 9, p. 1251-1259 9 p.

Research output: Contribution to journal › Journal article › Research › peer-review

9 Citations (Scopus)

2000

Structural basis for the catalytic mechanism of a proficient enzyme: Orotidine 5'-Monophosphate Decarboxylase

Harris, P. H., Poulsen, J-C. N., Jensen, K. F. & Larsen, S., 2000, In: Biochemistry. 39, 15, p. 4217-4224

Research output: Contribution to journal › Journal article › Research › peer-review

93 Citations (Scopus)

1999

Crystallization and preliminary X-ray studies of β-1,4-galactanase from Aspergillus aculeatus

Ryttersgaard, C., Poulsen, J-C. J., Christgau, S., Sandal, T., Dalbøge, H. & Larsen, S., 1999, In: Acta Cryst.. D55, p. 929-930

Research output: Contribution to journal › Journal article › Research › peer-review

1998

Crystallization and preliminary X-ray diffraction studies of the heterogeneously glycosylated enzyme rhamnogalacturonan acetylesterase from Aspergillus aculeatus

Mølgaard, A., Petersen, J. F. W., Kauppinen, S., Dalbøge, H., Johnsen, A. H., Poulsen, J-C. N. & Larsen, S., 1998, In: Acta Crystallographica. D54, p. 1026-1029

Research output: Contribution to journal › Journal article › Research › peer-review

1995

Thapsigargin-sensitive Ca²⁺-ATPases account for Ca²⁺ uptake to inositol 1,4,5-trisphosphate-sensitive and caffeine-sensitive Ca²⁺ stores in adrenal chromaffin cells

Poulsen, J-C. N., Caspersen, C., Mathiasen, D., East, J. M., Tunwell, R. E. A., Lai, F. A., Maedea, N., Mikoshiba, K. & Treiman, M., 1995, In: Biochem. J.. 307(3), p. 749-758

Research output: Contribution to journal › Journal article › Research

1994

Ca²⁺-ATPase inhibitory activity of a locked analog of thapsigargin

Andersen, A., Treiman, M., Poulsen, J-C. N., Cornett, C., Moldt, P., Olsen, C. E. & Christensen, S. B., 1994, In: Bioorg. Med. Chem. Lett.. 4(5), p. 657-660

Research output: Contribution to journal › Journal article › Research › peer-review

5 Citations (Scopus)

1993

Derivatives of thapsigargin as probes of its binding-site on endoplasmicreticulum Ca²⁺ ATPase stereoselectivity and important functional groups

Christensen, S. B., Andersen, A., Poulsen, J-C. N. & Treiman, M., 1993, In: FEBS Letters. 335(3), p. 345-348

Research output: Contribution to journal › Journal article › Research › peer-review

Jens-Christian Navarro Poulsen

Research output

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