Jette Sandholm Jensen Kastrup

Primary fields of research

Jette Sandholm Kastrup function as group leader on a Biostructural Research core project on ion channel receptors (ionotropic glutamate receptors and Cys-loop receptors).

The group is one of the leading groups in this field of ionotropic glutamate receptors and has demonstrated its expertise in analyzing the mechanisms of compound action at iGluRs, using X-ray crystallography and various methods of protein analysis. Moreover, the group was the first to determine the structure of the LBD of the AMPA receptor GluA4 (Kasper et al., 2008), the kainate receptor GluK1 (Naur et al., 2005), the iGluR orphan receptor GluD2 (Naur et al., 2007) and the kainate receptor GluK3 (Venskutonyte et al., 2011).

CV

CV: Jette Sandholm Kastrup                                              

Work address:    Department of Drug Design and Pharmacology

                           Biostructural Research

                           Faculty of Health and Medical Sciences

                           University of Copenhagen

                           Universitetsparken 2, DK-2100 CPH Ø, Denmark

                           Phone: +45 3533 6486; E-mail: [email protected]

 Academic Degrees

1986: M.Sc. (pharm.), The Royal Danish School of Pharmacy (RDSP)

1988: PhD, RDSP & Cheminova Agro A/S

Considered professorable 2002 (”well qualified, broad crystallographic expertise, experience in leading a successful research group, research activities on a broad spectrum of biologically important problems”). After application of professorship within protein crystallography at the Danish University of Pharmaceutical Sciences

Employments

1981-1982: 11 Months work experience at Tårnby Apotek

1982-1986: Periodic substitute at Tårnby Apotek

1986-1988: Industrial PhD student (Erhvervsforsker). RDSP & Cheminova Agro

1988: Teaching assistant. Dept. of Organic Chemistry, RDSP (3 months)

1989-1992: Post doc. Dept. of Medicinal Chemistry, RDSP

1993-1994: Senior scientist. Dept. of Medicinal Chemistry, RDSP

1995-2008: Associate professor. Dept. of Medicinal Chemistry, The Danish University of Pharmaceutical Sciences (DUPS)

2008-present: Professor (MSO) in structure-based drug design. Dept. of Drug Design and Pharmacology, The Faculty of Health and Medical Sciences, University of Copenhagen

Stays as visiting research fellow

1989-1991: Novo Nordisk A/S, dr. Hans Flodgaard. Protein purification (in total 2 months)

1990: University of Oregon, USA, Professor Brian W. Matthews. Proteinmcrystallography (1 month)

1991: University of Oregon, USA, Professor Brian W. Matthews. Protein crystallography (2 weeks)

1991: Uppsala University, Sweden, Dr. Elisabeth S. Eriksson. Protein crystallography (1 week)

1992: DAKO A/S, Dr. Inge Clemmensen. Protein purification (3 months)

Maternity leaves

1991: 6 months (January-June)

1997-1998: 7 months (November 1997 – May 1998)

Research prizes

45.000 kr. from ”Valborg and Edith Larsens Foundation" 2005

20.000 kr from the Faculty of Pharmaceuitcal Sciences, University of Copenhagen 2007    

Major Administrative Responsibilities

1999-2002: Member of Board, the Dansync Center for synchrotron radiation

2002-2008: Member of departmental board, Dept. of Medicinal Chemistry, DUPS

2004-2008: Deputy Head of Dept. of Medicinal Chemistry. DUPS and the Faculty of Pharmaceutical Sciences, University of Copenhagen

2004-2006: Member of Academic Council, DUPS

2004-2006: Member of committee for defining central and prioritized research areas, DUPS

2004-2006: Member of committee for evaluation of VIP assessment committees, DUPS

2005-2007: Member of scientific committee, Dept. of Medicinal Chemistry, DUPS

2007-2011: Member of Academic Council, Faculty of Pharmaceutical Sciences, University of Copenhagen

2007: Appointed director of Drug Research Academy, an industry-oriented research education programme at the Faculty of Pharmaceutical Sciences, University of Copenhagen (August-October)

2007: Member of committee for preparation of strategy plan 2007-2010, Faculty of Pharmaceutical Sciences, University of Copenhagen

2007-2008: Participant in project on competence development, The Ministry of Science, Technology and Innovation (3 days)

2007-2012: Director of Drug Research Academy

2008-present: Head of GluTarget, project within the Programme of Excellence at the University of Copenhagen (2008-2013). 24.5 mio. kr. Project title: Ionotropic glutamate receptors as targets for structure-based drug research: A multidisciplinary programme in structural biology, chemical biology, medicinal chemistry, molecular pharmacology and ex vivo/in vivo pharmacology

2010-2012: Member of board, SHARE's Cross Faculty PhD Initiative, University of Copenhagen

2010-2012: Coordinator of mobility PhD grant on project “Ion channel receptors in brain diseases”

2012: Member of Academic Council, Faculty of Health and Medical Sciences, University of Copenhagen

2012-present: Member of Research and Innovation Committee, Dept. of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen

Organizer of symposia

Co-organizer of Alfred Benzon Symposium no. 39, 1995: Structure and Function of 7TM Receptors

Co-Organizer of 9. CoLuAa-meeting, Royal Danish School of Pharmacy, 16-17 Nov. 2000 (between protein crystallographers at Lund, Copenhagen and Aarhus Universities and Novo Nordisk A/S, Carlsberg Laboratorium and Royal Danish School of Pharmacy)

Organizer of symposium on Receptor Structure and Function, Royal Danish School of Pharmacy 2001

Organizer of DANSYNC annual meeting, Royal Danish School of Pharmacy, 23. May 2001

Organizer of symposium on Receptor Structure and Function, Danish University of Pharmaceutical Sciences 2002

Organizer of mini-symposium on Protein Crystallography in Structural Biology, Danish University of Pharmaceutical Sciences, 18. June 2002

Organizer of symposium on Receptor Structure and Function, Danish University of Pharmaceutical Sciences 2003

Co-organizer of the 33. Danske Krystallograf/5. DANSYNC meeting, Danish University of Pharmaceutical Sciences, May 27-28 2003

Organizer of symposium on Receptor Structure and Function, Danish University of Pharmaceutical Sciences 2004

Organizer of symposium on Receptor Structure and Function, Danish University of Pharmaceutical Sciences 2005

Organizer of symposium on Receptor Structure and Function, Danish University of Pharmaceutical Sciences 2006

Co-organizer of Academic Council strategy seminar, Danish University of Pharmaceutical Sciences 2006

Co-Organizer of the 15. CoLuAa meeting, Danish University of Pharmaceutical Sciences, Nov. 9-10 2006

Organizer of symposium on Receptor Structure and Function, Faculty of Pharmaceutical Sciences, University of Copenhagen 2007

Organizer of symposium on Receptor Structure and Function, Faculty of Pharmaceutical Sciences, University of Copenhagen 2008

Organizer of Symposium on iGluRs, Faculty of Pharmaceutical Sciences, University of Copenhagen, November 2008

Organizer of GluTarget General Meeting for members, May 2009

Organizer of symposium on Receptor Structure and Function, Faculty of Pharmaceutical Sciences, University of Copenhagen 2009

Organizer of symposium on Receptor Structure and Function, Faculty of Pharmaceutical Sciences, University of Copenhagen 2010

Organizer of GluTarget Day of Research, April 2010

Organizer of symposium with presentations by the members of the Advisory Board and GluTarget members, June 2010

Organizer of symposium on Receptor Structure and Function, Faculty of Pharmaceutical Sciences, University of Copenhagen 2011

Organizer of GluTarget symposium on ionotropic glutamate receptors, February 2012

Organizer of symposium on Receptor Structure and Function, Faculty of Health and Medical Sciences, University of Copenhagen 2012

Organizer of symposium with presentations by the members of the Advisory Board and GluTarget members, October 2012

Organizer of symposium on Receptor Structure and Function, Faculty of Health and Medical Sciences, University of Copenhagen 2013

Reviewer at several international journals and of international grant applications

Conferences and symposia

Participation in numerous conferences and meetings. Numerous poster presentations with abstracts. Examples of invited speaker within recent years at international conferences:

Gordon Conference on Excitatory synapses & brain function, Colby-Sawyer College, New London, NH, June 10-15, 2007

Physiology 2010 Conference, University Place, Manchester, UK, June 30-July 2, 2010

20th Neuropharmacology Conference - High Resolution Neuropharmacology: Structure Changes the Paradigm, San Diego, USA, November 10-12, 2010

Membrane Proteins:  Structure & Function,Lady Margaret Hall, Oxford, April 6-8, 2011

Glutamatergic Synapse ISN Satelite meeting, Crete Island, Greece, September 2-5, 2011

Present central research projects

Group leader on BR core project on ion channel receptors (ionotropic glutamate receptors and Cys-loop receptors)

Active in BR core project on Chromatin modifying enzymes

Expertise

Structural biology, protein crystallography

National and international collaborations on iGluRs

Emory University School of Medicine, Georgia, USA

Northwestern Univ. Feinberg School of Medicine, Illinois, USA     

University of Sienna, Italy

University of Jyväskylä, Finland

Oregon Health and Science University, Oregon, USA

Dartmouth Medical School, New Hampshire, USA

Yokohama City University, Japan

University of Liege, Belgium

Neurosearch A/S, Denmark

H. Lundbeck A/S, Denmark

The Faculty of Science, University of Copenhagen, Denmark

National and international collaborations on Cys-loop receptors

University of Sydney, Australia

University of Milano, Italy

Aniona, Denmark

Grants – as main applicant (excl. internal and industrial PhD stipends)

Alfred Benzon's Fond; Augustinus Foundation; Carlsberg Foundation; Danish Agency for Science Technology and Innovation; Danish Council for Independent Research, Medical Sciences; Danish Council for Independent Research, Natural Sciences; Danish Research Academy;Hede Nielsen Foundation; Lundbeck Foundation; Novo Nordisk A/S; Programme of Excellence at the University of Copenhagen

Grants – as co-applicant

Apotekerfonden of 1991; Copenhagen 2016, University of Copenhagen; Danish Agency for Science, Technology and Innovation; Danish Medical Research Council; Danish Natural Science Research Council; Ib Henriksens Fond; Lundbeck Foundation, Novo Nordisk Fond

Publications 1988-2013

Peer-reviewed: 93

            1990-1994: 4

         1995-1999: 12

         2000-2004: 17

         2005-2009: 37

         2010-May 2013: 23

Books (editor, chapters): 7

Patents: 2

Other publications: 28

PhD thesis: 1

Structures deposited in the Protein Data Bank: 106

Current research

The current research of Jette Sandholm Kastrup focuses on:
Ion channel receptors in the central nervous system (main project)

• Ionotropic glutamate receptors
• Cys-loop receptors

Chromatin modifying enzymes

Teaching

Teaching CV: Jette Sandholm Kastrup                                             

Work address:    Department of Drug Design and Pharmacology

                           Biostructural Research

                           Faculty of Health and Medical Sciences

                           University of Copenhagen

                           Universitetsparken 2, DK-2100 CPH Ø, Denmark

                           Phone: +45 3533 6486; E-mail: [email protected]

Teaching at undergraduate courses 1988-2013

Introduction to study (1. semester: lectures, lab courses, project work, planning and evaluation)

Organic chemistry (1.-3. semester: teaching in class-room, lab courses, planning and evaluation)

Biochemistry (2. semester: lectures)

Bioorganic chemistry (3. semester: lectures)

Interdisciplinary course (5. semester: project work)

Basic drug design and discovery (6. or 7. semester: lectures, exams, planning and evaluation)

Bioinformatics and structural biology (7. semester: lectures, computer workshops, exams, planning and evaluation)

Medicinal and biostructural chemistry (7. semester: lectures, computer workshops, exams, planning and evaluation)

Structural chemistry (9. semester: lectures, computer workshops, project supervisions, exams, planning and evaluation)

Structural and computational medicinal chemistry (9. semester: lectures, computer workshops, project supervisions, planning and evaluation)

Teaching at PhD courses 1994-2013

Advanced Medicinal Chemistry and Drug Design, Royal Danish School of Pharmacy, 1994-1998 (lectures and workshop)

Cross-Institutional PhD Course on Molecular Biophysics, Royal Danish School of Pharmacy, 2000 (lecture)

Drug Design and Discovery, Royal Danish School of Pharmacy, 1999-2000 (lectures).

Protein Structure and Interaction, Panum Institute, 1995 (lecture)

Advanced Structural Chemistry and Molecular Modelling, Royal Danish School of Pharmacy, 1994-2001 and Danish University of Pharmaceutical Sciences, 2002-2007 (lectures, computer workshops, planning and evaluation)

Analytical Challenges in the Formulation Design of Pharmaceutical Proteins, Royal Danish School of Pharmacy, 2002 and Faculty of Pharmaceutical Sciences, University of Copenhagen 2007, 2009 and 2011 (lectures)

Biostructures and Molecular Modeling in Drug Research, Faculty of Pharmaceutical Sciences, University of Copenhagen, 2008, 2009 and 2011 (lectures, computer workshops, planning and evaluation)

Receptor Structure and Function, Royal Danish School of Pharmacy, 1999-2002, Danish University of Pharmaceutical Sciences, 2002-2006, Faculty of Pharmaceutical Sciences, University of Copenhagen, 2007-2011 and Faculty of Health and Medical Sciences, University of Copenhagen 2012-2013 (course director, lectures, workshops, exams, planning and evaluation)

Biostructures and Molecular Modeling in Drug Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2012 and 2013 (lectures)

Organizer and teaching at Summer Schools 1997-2009

Third ULLA Summer School (LACDR) 1997, TEC4: Purification of proteins, including antibodies (lecture, workshop, planning and evaluation)

Fourth ULLA Summer School (Copenhagen) 1999, course no. 21:  Purification of proteins, including antibodies and recombinant proteins (lecture, workshop, planning and evaluation)

Fifth ULLA Summer School (London) 2001, course no. 17: Applied protein purification and characterisation techniques for production, crystallisation and proteomics (lectures, workshop, planning and evaluation)

Sixth ULLA Summer School for Postgraduate Training and Research (Paris) 2003, course no. 17: Applied Protein Purification and Characterisation (lectures, workshop, planning and evaluation)

DRA Summer School 2008: Research planning and individual project management (organizer)

Ninth ULLA Summer School for Postgraduate Training and Research (Copenhagen) 2009: Course on “Three-dimensional protein structures in drug research” (lecture, workshop, planning and evaluation)

Ninth ULLA Summer School for Postgraduate Training and Research (Copenhagen) 2009: Lecture on “Structure-based drug design and virtual screening of drug libraries” (lecture)

DRA Summer School 2009: Career opportunities and new trends (organizer)

DRA Summer School 2010: Drug development and discovery (organizer)

DRA Summer School 2011: Biomarkers and regulatory affairs (organizer)

DRA Summer School 2012: Biologics, ethics and presentation skills (organizer)

Training of high school teachers in biotechnology 2010-2012

Course on Biostructure and Biofunction, 2010, 2011 and 2012 (in total 8 one-day courses including lectures, lab exercises, computer exercises, planning and evaluation – more than 200 teachers)

Research dissemination

”Lægemidler mod hjernens sygdomme”. Lectures offered to high school students.

Examiner

- at several national and international MSc project work and Ph.D. defenses, 1997-2013.

Production of candidates (MSc thesis work) 1993-2013

1993. Project: Røntgenkrystallografi. Krystallisation og structuropkalring af småmolekyler. Krystallisation af recombinant humant væksthormon

1995. Project: Project in X-ray crystallography

2004. Project: Structural and pharmacological study of the partial selective agonist CPW-399

2004: Project: B cell linker protein. Cloning and expression in E. coli and Drosophila S2 cells

2005. Project: X-ray crystallography studies of ionotropic glutamate receptor type 2 – An important player in the CNS

2006. Project: Kloning og homologibygning af kainatreceptorer

2007. Project: Membrane peptide transporter – YgdR. Expression and purification of YdgR in E. coli

2008. Project: Krystallization af GluR6’s ligandbindende domæne og cloning af GluR7’s ligandbindende domæne

2008. Project: Expression and purification of PDZ domains for biophysical and structural studies

2008. Project: Double mutant of the ligand-binding domain of iGluR2, Leu483Tyr and Asn754Ser

2009. Project: Structure determination of a double mutant of the ionotropic glutamate receptor iGluR2 in order to characterize the binding of positive allosteric modulators Diazoxide and Cyclothiazide

2009. Project: Structure determination of the iGluR2 and iGluR5 crystallized under the presence of L-aspartate and kainate respectively

2009. Project: Cloning, expression and purification of the extracellular part of the cys-loop receptors GABA rho-2 and acetylcholine binding protein in the pichia pastoris expression system and using the baculovirus expression vector system and insect cells

2009. Project: Expression, purification and crystallization of Jumonji C domain containing histone demethylases JMJD2A and JMJD2C

2010. Project: Cloning, expression and purification of the N-terminal domain of the ionotropic glutamate receptor subunit, GluN1-1a

2010. Project: Cloning, expression, purification and crystallization of the histone demethylase JMJD2C

2010: Project: Structure determination of PSD95-PDZ12

2010. Project: Preparation of the ligand binding domain of the NMDA receptor subunit GluN1 for structural studies

2011: Project: Structural studies of the N-terminal domain of ionotropic glutamate receptors

2012. Project: Studies of the Function of the Plant Homeo Domains of the Histone Demethylase KDM5B

2012. Project: Screening, characterization and crystallization of histone demethylase inhibitors with ccKDM4A and ccKDM4C

2012. Project: Structural studies of the kainate receptor GluK3 ligand-binding domain in complex with two ligands – ES284 and ZA302

2013: Project: Structural studies of AMPA and kainate receptor ligand-binding domains in complex with new antagonists

2013: Project: Structural basis for understanding the functional role of the ligand-binding domain of the GluD2 receptor

PhD supervisor functions 1994-2013

PhD scholarship 1994-1997. Project: Heparin binding protein

PhD scholarship 1996-2000. Project: Structure-function studies of heparin-binding protein (HBP/CAP37/azurocidin) (main supervisor (MS))

PhD scholarship 1998-2001. Project: From gene to crystal structure: a study of the ligand binding domain of AMPA and kainate receptors (MS)

PhD scholarship 1999-2002. Project: Studies on receptor-ligand interactions of ionotropic glutamate receptors by X-ray crystallography and molecular modelling

PhD scholarship 2000-2003. Project: DNA recognition of peptide nucleic acids (PNA): Structural studies by X-ray crystallography

PhD scholarship 2000-2003. Project: Structural studies of protein tyrosine phosphatases by X-ray crystallography (MS)

PhD scholarship 2002-2005. Project: Mechanisms for activation and antagonism of ionotropic glutamate receptors: Studies of protein-ligand interactions by protein crystallography and electrophysiology (MS)

PhD scholarship 2003-2006. Project: Characterization of the role of Vav1 in the mast cell signalosome

PhD scholarship 2005-2007. Project: Structural studies of cellular signaling (MS)

Industrial PhD scholarship 2005-2008. Project: Modulation of AMPA receptor deactivation: Structural and electrophysiological studies (MS)

PhD scholarship 2007-2009. Project: Structural and functional studies of the neuronal D-serine transporter asc-1 (MS)

Industrial PhD scholarship 2008-2010. Project: Structural and electrophysiological studies of Cys-loop receptors (MS)

PhD scholarship 2008-2011: Project: The C-terminal domain of ionotropic glutamate receptors as targets for modulation of protein-protein interactions

PhD scholarship 2008-2011: Project: The structural basis of histone demethylases as cancer drug targets

PhD scholarship 2008-2011: Project: Pharmacological and structural characterization of kainate receptors

PhD scholarship 2009-2012: Project: Ionotropic glutamate receptors as drug targets. Structural studies of the N-terminal domain

PhD scholarship 2009-2012: Project: Cys-loop receptors: structural studies of GABA rho receptors and the Acetylcholine binding protein

PhD scholarship 2010-2013: Project: Structural and functional studies of histone lysine demethylase subfamilies KDM6 and KDM4

PhD scholarship 2010-2013: Project: Structural basis for understanding the role of the ligand binding core of the orphan delta2 receptor in function and development of ligands (MS)

PhD scholarship 2010-2013: Project: Structure-activity relationships of allosteric modulators of GluA2: Studies using X-ray crystallography, isothermal titration calorimetry, and molecular modeling

PhD scholarship 2010-2013: Project: Acetylcholine binding protein as surrogate for the nicotinic acetylcholine alpha4beta2 receptor

Industrial PhD scholarship 2011-2013: Project: Structural and electrophysiological studies of nicotinic acetylcholine receptors with focus on allosteric modulators (MS)

PhD scholarship 2012-2015: Project: Molecular basis of selective modulation of ionotropic glutamate receptors (MS)

Post docs 2005-2013

In total 7

Courses on pedagogy

Pedagogic Course, 1993

Mentor seminar, 2001

Course on supervision of PhD students, University of Copenhagen, 2010-2011

Course for academic advisors at LIFE, FARMA, SCIENCE AND HEALTH, Department of Science Education (DSE), Faculty of Science, University of Copenhagen, April 15, 2011

English language competence assessment for lecturers at FARMA, course at the Faculty of Pharmaceutical Sciences, University of Copenhagen, December 2011

Participation in seminars on pedagogy arranged by the Royal Danish School of Pharmacy and the Danish University of Pharmaceutical Sciences

Committees

Member of committee for pedagogy/education, Royal Danish School of Pharmacy: 1996-1999

Member of IT political contact organ for university institutions: 1998

Member of mentor committee for pedagogy/education, Royal Danish School of Pharmacy: 2001-2002; Danish University of Pharmaceutical Sciences: 2002-2006; Faculty of Pharmaceutical Sciences, University of Copenhagen: 2007-2011; and Faculty of Health and Medical Sciences: 2012-2013 (training on teaching as part of course for post docs/assistant professors)

Member of committee concerning toxicology and work environment, Royal Danish School of Pharmacy: 2000-2002

Member of committee concerning internship period at a community (or hospital) pharmacy, Danish University of Pharmaceutical Sciences: 2003

Introductory remarks on publicationslist

Publications 1988-2013 by Jette Sandholm Kastrup

Peer-reviewed: 93

    1990-1994: 4
    1995-1999: 12
    2000-2004: 17
    2005-2009: 37
    2010-May 2013: 23

Books (editor, chapters): 7
Patents: 2
Other publications: 28
PhD thesis: 1
Structures deposited in the Protein Data Bank: 106