Imaging of DNA Ultrafine Bridges in Budding Yeast

Oliver Quevedo Rodriguez, Michael Lisby

Abstract

DNA ultrafine bridges (UFBs) are a type of chromatin-free DNA bridges that connect sister chromatids in anaphase and pose a threat to genome stability. However, little is known about the origin of these structures, and how they are sensed and resolved by the cell. In this chapter, we review tools and methods for studying UFBs by fluorescence microscopy including chemical and genetic approaches to induce UFBs in the model organism Saccharomyces cerevisiae.

OriginalsprogEngelsk
TitelGenome Instability : Methods and Protocols
RedaktørerMarco Muzi-Falconi, Grant W. Brown
Antal sider11
ForlagHumana Press
Publikationsdato2018
Sider483-493
ISBN (Trykt)978-1-4939-7305-7
ISBN (Elektronisk)978-1-4939-7306-4
DOI
StatusUdgivet - 2018
NavnMethods in Molecular Biology
Vol/bind1672
ISSN1064-3745

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