Imaging of DNA Ultrafine Bridges in Budding Yeast

Oliver Quevedo Rodriguez, Michael Lisby

Abstract

DNA ultrafine bridges (UFBs) are a type of chromatin-free DNA bridges that connect sister chromatids in anaphase and pose a threat to genome stability. However, little is known about the origin of these structures, and how they are sensed and resolved by the cell. In this chapter, we review tools and methods for studying UFBs by fluorescence microscopy including chemical and genetic approaches to induce UFBs in the model organism Saccharomyces cerevisiae.

Original languageEnglish
Title of host publicationGenome Instability : Methods and Protocols
EditorsMarco Muzi-Falconi, Grant W. Brown
Number of pages11
PublisherHumana Press
Publication date2018
Pages483-493
ISBN (Print)978-1-4939-7305-7
ISBN (Electronic)978-1-4939-7306-4
DOIs
Publication statusPublished - 2018
SeriesMethods in Molecular Biology
Volume1672
ISSN1064-3745

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