Engineered CHO cells for production of diverse, homogeneous glycoproteins

TY - JOUR

T1 - Engineered CHO cells for production of diverse, homogeneous glycoproteins

AU - Yang, Zhang

AU - Wang, Shengjun

AU - Halim, Adnan

AU - Schulz, Morten Alder

AU - Frödin, Morten

AU - Rahman, Shamim H

AU - Vester-Christensen, Malene Bech

AU - Behrens, Carsten

AU - Kristensen, Claus

AU - Vakhrushev, Sergey Y

AU - Bennett, Eric Paul

AU - Wandall, Hans H

AU - Clausen, Henrik

PY - 2015/8/10

Y1 - 2015/8/10

N2 - Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.

AB - Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.

U2 - 10.1038/nbt.3280

DO - 10.1038/nbt.3280

M3 - Journal article

C2 - 26192319

SN - 1087-0156

VL - 33

SP - 842

EP - 844

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 8

ER -