Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract

Mette Skau Mikkelsen; Sebastian Meier; Morten G Jensen; Fen Qin; Iuliana-Madalina Stoica; Helle Juel Martens; Andreas Blennow; Birthe P Møller Jespersen

doi:10.1016/j.jcs.2017.02.003

Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract

Mette Skau Mikkelsen^*, Sebastian Meier, Morten G Jensen, Fen Qin, Iuliana-Madalina Stoica, Helle Juel Martens, Andreas Blennow, Birthe P Møller Jespersen

^*Corresponding author af dette arbejde

6 Citationer (Scopus)

51 Downloads (Pure)

Abstract

β-Glucan dietary fiber rich food systems hold a great potential for improving human health. Here, we evaluate the extractability and quality of β-glucan liquid formulations from eight different barley genotypes varying in β-glucan content and molecular structure using Termamyl^® SC (T), Attenuzyme^® (A) and Attenuzyme^® Flex (AF) amylolytic enzymes in combinations. Extracts from barley lines Lys5f, KVL408, KVL1104 and CDC Fibar exceeded 4 g β-glucan/l, providing European Food Safety Authority (EFSA) and U.S. Food and Drug Administration (FDA) recommended amounts (3 g β-glucan/day) from three portions. TAF extracts of Lys5f and KVL408 grains reached extraordinary high concentrations of 8–9 g β-glucan/l. The β-glucan molecular mass decreased with enzyme treatment T < TA < TAF due to minor lichenase side activity. Extractability was generally higher and molecular mass lower for barley lines low in triosyl/tetraosyl (DP3/DP4) ratios than for those high in DP3/DP4 ratios (Lys5f, KVL408 and KVL1104). Overall, the higher β-glucan content and structural robustness in Lys5f and KVL408 raw materials favor these in a β-glucan rich extract with potential for EFSA and FDA health and nutrition claims.

Originalsprog	Engelsk
Tidsskrift	Journal of Cereal Science
Vol/bind	75
Sider (fra-til)	45-53
Antal sider	9
ISSN	0733-5210
DOI	https://doi.org/10.1016/j.jcs.2017.02.003
Status	Udgivet - 2017

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10.1016/j.jcs.2017.02.003

Barley_genotypic_β-glucan_variation_2017_acceptedAccepteret manuskript, 1,98 MBLicens: CC BY-NC-ND

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title = "Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract",

abstract = "β-Glucan dietary fiber rich food systems hold a great potential for improving human health. Here, we evaluate the extractability and quality of β-glucan liquid formulations from eight different barley genotypes varying in β-glucan content and molecular structure using Termamyl{\textregistered} SC (T), Attenuzyme{\textregistered} (A) and Attenuzyme{\textregistered} Flex (AF) amylolytic enzymes in combinations. Extracts from barley lines Lys5f, KVL408, KVL1104 and CDC Fibar exceeded 4 g β-glucan/l, providing European Food Safety Authority (EFSA) and U.S. Food and Drug Administration (FDA) recommended amounts (3 g β-glucan/day) from three portions. TAF extracts of Lys5f and KVL408 grains reached extraordinary high concentrations of 8–9 g β-glucan/l. The β-glucan molecular mass decreased with enzyme treatment T < TA < TAF due to minor lichenase side activity. Extractability was generally higher and molecular mass lower for barley lines low in triosyl/tetraosyl (DP3/DP4) ratios than for those high in DP3/DP4 ratios (Lys5f, KVL408 and KVL1104). Overall, the higher β-glucan content and structural robustness in Lys5f and KVL408 raw materials favor these in a β-glucan rich extract with potential for EFSA and FDA health and nutrition claims.",

keywords = "Barley beta-glucans, Enzymatic hydrolysis, Extractability, Molecular structure",

author = "Mikkelsen, {Mette Skau} and Sebastian Meier and Jensen, {Morten G} and Fen Qin and Iuliana-Madalina Stoica and Martens, {Helle Juel} and Andreas Blennow and Jespersen, {Birthe P M{\o}ller}",

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journal = "Journal of Cereal Science",

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T1 - Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract

AU - Mikkelsen, Mette Skau

AU - Meier, Sebastian

AU - Jensen, Morten G

AU - Qin, Fen

AU - Stoica, Iuliana-Madalina

AU - Martens, Helle Juel

AU - Blennow, Andreas

AU - Jespersen, Birthe P Møller

PY - 2017

Y1 - 2017

N2 - β-Glucan dietary fiber rich food systems hold a great potential for improving human health. Here, we evaluate the extractability and quality of β-glucan liquid formulations from eight different barley genotypes varying in β-glucan content and molecular structure using Termamyl® SC (T), Attenuzyme® (A) and Attenuzyme® Flex (AF) amylolytic enzymes in combinations. Extracts from barley lines Lys5f, KVL408, KVL1104 and CDC Fibar exceeded 4 g β-glucan/l, providing European Food Safety Authority (EFSA) and U.S. Food and Drug Administration (FDA) recommended amounts (3 g β-glucan/day) from three portions. TAF extracts of Lys5f and KVL408 grains reached extraordinary high concentrations of 8–9 g β-glucan/l. The β-glucan molecular mass decreased with enzyme treatment T < TA < TAF due to minor lichenase side activity. Extractability was generally higher and molecular mass lower for barley lines low in triosyl/tetraosyl (DP3/DP4) ratios than for those high in DP3/DP4 ratios (Lys5f, KVL408 and KVL1104). Overall, the higher β-glucan content and structural robustness in Lys5f and KVL408 raw materials favor these in a β-glucan rich extract with potential for EFSA and FDA health and nutrition claims.

AB - β-Glucan dietary fiber rich food systems hold a great potential for improving human health. Here, we evaluate the extractability and quality of β-glucan liquid formulations from eight different barley genotypes varying in β-glucan content and molecular structure using Termamyl® SC (T), Attenuzyme® (A) and Attenuzyme® Flex (AF) amylolytic enzymes in combinations. Extracts from barley lines Lys5f, KVL408, KVL1104 and CDC Fibar exceeded 4 g β-glucan/l, providing European Food Safety Authority (EFSA) and U.S. Food and Drug Administration (FDA) recommended amounts (3 g β-glucan/day) from three portions. TAF extracts of Lys5f and KVL408 grains reached extraordinary high concentrations of 8–9 g β-glucan/l. The β-glucan molecular mass decreased with enzyme treatment T < TA < TAF due to minor lichenase side activity. Extractability was generally higher and molecular mass lower for barley lines low in triosyl/tetraosyl (DP3/DP4) ratios than for those high in DP3/DP4 ratios (Lys5f, KVL408 and KVL1104). Overall, the higher β-glucan content and structural robustness in Lys5f and KVL408 raw materials favor these in a β-glucan rich extract with potential for EFSA and FDA health and nutrition claims.

KW - Barley beta-glucans

KW - Enzymatic hydrolysis

KW - Extractability

KW - Molecular structure

U2 - 10.1016/j.jcs.2017.02.003

DO - 10.1016/j.jcs.2017.02.003

M3 - Journal article

AN - SCOPUS:85016141146

SN - 0733-5210

VL - 75

SP - 45

EP - 53

JO - Journal of Cereal Science

JF - Journal of Cereal Science

ER -

Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract

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