Yeast species associated with different wine grape varieties in Denmark

TY - JOUR

T1 - Yeast species associated with different wine grape varieties in Denmark

AU - Lederer, Michaela Anna

AU - Nielsen, Dennis Sandris

AU - Toldam-Andersen, Torben Bo

AU - Herrmann, J.V.

AU - Arneborg, Nils

PY - 2013/1

Y1 - 2013/1

N2 - Yeasts naturally occurring on different grape varieties, grown in Denmark, were isolated from two interspecific grape varieties and one hybrid in the years 2008 and 2009 from three different vineyards. The analysed grapes harbored a total yeast plate count of 103-104 CFU/ml. A total of 200 isolates were grouped according to their rep-PCR profiles and representative isolates identified by sequencing of the 26S rRNA D1/D2 region and selected phenotypic tests. The yeast microflora on the grapes was dominated by non-Saccharomyces yeasts. Twelve different yeast species belonging to six genera were detected, including Rhodotorula fujisanensis, Rhodotorula glutinis, Rhodotorula nothofagi, Cryptococcus carnescens, Cryptococcus festucosus, Cryptococcus spp., Cryptococcus victoriae, Cryptococcus wieringae, Hanseniaspora uvarum, Metschnikowia fructicola, Kazachstania servazzii and Sporobolomyces roseus and the yeast-like ascomycetous fungi, Aureobasidium pullulans. To our knowledge, this study is the first of its kind reporting on the yeast composition of grapes grown in Denmark, and thus providing important information for the production of high-quality Danish wines.

AB - Yeasts naturally occurring on different grape varieties, grown in Denmark, were isolated from two interspecific grape varieties and one hybrid in the years 2008 and 2009 from three different vineyards. The analysed grapes harbored a total yeast plate count of 103-104 CFU/ml. A total of 200 isolates were grouped according to their rep-PCR profiles and representative isolates identified by sequencing of the 26S rRNA D1/D2 region and selected phenotypic tests. The yeast microflora on the grapes was dominated by non-Saccharomyces yeasts. Twelve different yeast species belonging to six genera were detected, including Rhodotorula fujisanensis, Rhodotorula glutinis, Rhodotorula nothofagi, Cryptococcus carnescens, Cryptococcus festucosus, Cryptococcus spp., Cryptococcus victoriae, Cryptococcus wieringae, Hanseniaspora uvarum, Metschnikowia fructicola, Kazachstania servazzii and Sporobolomyces roseus and the yeast-like ascomycetous fungi, Aureobasidium pullulans. To our knowledge, this study is the first of its kind reporting on the yeast composition of grapes grown in Denmark, and thus providing important information for the production of high-quality Danish wines.

U2 - 10.1080/09064710.2012.723738

DO - 10.1080/09064710.2012.723738

M3 - Journal article

SN - 0906-4710

VL - 63

SP - 89

EP - 96

JO - Acta Agriculturae Scandinavica. Section B. Soil and Plant Science

JF - Acta Agriculturae Scandinavica. Section B. Soil and Plant Science

IS - 1

ER -