Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group

Walther Parson; Harald Niederstätter; Alexandra Lindinger; Peter Gill; Niels Morling

doi:10.1016/j.fsigen.2007.12.004

Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group

Walther Parson, Harald Niederstätter, Alexandra Lindinger, Peter Gill, Niels Morling

Section of Forensic Genetics

10 Citations (Scopus)

Abstract

The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA.

Original language	English
Journal	Forensic Science International: Genetics
Volume	2
Issue number	3
Pages (from-to)	238-42
Number of pages	4
ISSN	1872-4973
DOIs	https://doi.org/10.1016/j.fsigen.2007.12.004
Publication status	Published - 2008

Keywords

Faculty of Health and Medical Sciences

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10.1016/j.fsigen.2007.12.004

Cite this

@article{a83bbb306bd011de8bc9000ea68e967b,

title = "Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group",

abstract = "The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA.",

keywords = "Faculty of Health and Medical Sciences",

author = "Walther Parson and Harald Niederst{\"a}tter and Alexandra Lindinger and Peter Gill and Niels Morling",

note = "Keywords: Bacteriophage lambda; Chromosomes, Human, Y; Complex Mixtures; DNA; DNA Fingerprinting; DNA, Viral; Forensic Genetics; Genotype; Humans; Male; Microsatellite Repeats; Reference Standards; Sensitivity and Specificity; Specimen Handling",

year = "2008",

doi = "10.1016/j.fsigen.2007.12.004",

language = "English",

volume = "2",

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journal = "Forensic Science International: Genetics",

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T1 - Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group

AU - Parson, Walther

AU - Niederstätter, Harald

AU - Lindinger, Alexandra

AU - Gill, Peter

AU - Morling, Niels

N1 - Keywords: Bacteriophage lambda; Chromosomes, Human, Y; Complex Mixtures; DNA; DNA Fingerprinting; DNA, Viral; Forensic Genetics; Genotype; Humans; Male; Microsatellite Repeats; Reference Standards; Sensitivity and Specificity; Specimen Handling

PY - 2008

Y1 - 2008

N2 - The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA.

AB - The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA.

KW - Faculty of Health and Medical Sciences

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DO - 10.1016/j.fsigen.2007.12.004

M3 - Journal article

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SN - 1872-4973

VL - 2

SP - 238

EP - 242

JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

IS - 3

ER -