TY - JOUR
T1 - Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group
AU - Parson, Walther
AU - Niederstätter, Harald
AU - Lindinger, Alexandra
AU - Gill, Peter
AU - Morling, Niels
N1 - Keywords: Bacteriophage lambda; Chromosomes, Human, Y; Complex Mixtures; DNA; DNA Fingerprinting; DNA, Viral; Forensic Genetics; Genotype; Humans; Male; Microsatellite Repeats; Reference Standards; Sensitivity and Specificity; Specimen Handling
PY - 2008
Y1 - 2008
N2 - The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA.
AB - The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases a laboratory-specific optimization process is indicated to reach a comparable sensitivity for the analysis of minute amounts of DNA.
KW - Faculty of Health and Medical Sciences
U2 - 10.1016/j.fsigen.2007.12.004
DO - 10.1016/j.fsigen.2007.12.004
M3 - Journal article
C2 - 19083827
SN - 1872-4973
VL - 2
SP - 238
EP - 242
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
IS - 3
ER -