Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus

Jean-Christophe Meunier; Judith M Gottwein; Michael Houghton; Rodney S Russell; Suzanne U Emerson; Jens Bukh; Robert H Purcell

doi:10.1093/infdis/jir511

Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus

Jean-Christophe Meunier, Judith M Gottwein, Michael Houghton, Rodney S Russell, Suzanne U Emerson, Jens Bukh, Robert H Purcell

Department of Immunology and Microbiology

77 Citations (Scopus)

Abstract

We detected cross-reactive neutralizing antibodies (NtAb) against hepatitis C virus (HCV) in chimpanzees vaccinated with HCV-1 (genotype 1a) recombinant E1/E2 envelope glycoproteins. Five vaccinated chimpanzees, protected following HCV-1 challenge, were initially studied using the heterologous H77 (genotype 1a) HCVpp assay. All animals had developed NtAb after the second vaccination; 4 animals had reciprocal titers of =200 at the time of challenge. Using genotypes 1a-6a HCV pseudoparticles (HCVpp) and cell culture-derived HCV (HCVcc) assays, cross-reactive NtAb were detected against 1a, 4a, 5a, and 6a, with limited reactivity against 2a and 3a. Our study provides encouragement for the development of a recombinant envelope-based vaccine against hepatitis C.

Original language	English
Journal	Journal of Infectious Diseases
Volume	204
Issue number	8
Pages (from-to)	1186-90
Number of pages	5
ISSN	0022-1899
DOIs	https://doi.org/10.1093/infdis/jir511
Publication status	Published - 15 Oct 2011

Keywords

Animals
Antibodies, Neutralizing
Cross Reactions
Flow Cytometry
Genotype
Hepacivirus
Hepatitis C, Chronic
Pan troglodytes
Retrospective Studies
Vaccination
Vaccines, Synthetic
Viral Envelope Proteins
Viral Hepatitis Vaccines

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/infdis/jir511

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title = "Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus",

abstract = "We detected cross-reactive neutralizing antibodies (NtAb) against hepatitis C virus (HCV) in chimpanzees vaccinated with HCV-1 (genotype 1a) recombinant E1/E2 envelope glycoproteins. Five vaccinated chimpanzees, protected following HCV-1 challenge, were initially studied using the heterologous H77 (genotype 1a) HCVpp assay. All animals had developed NtAb after the second vaccination; 4 animals had reciprocal titers of =200 at the time of challenge. Using genotypes 1a-6a HCV pseudoparticles (HCVpp) and cell culture-derived HCV (HCVcc) assays, cross-reactive NtAb were detected against 1a, 4a, 5a, and 6a, with limited reactivity against 2a and 3a. Our study provides encouragement for the development of a recombinant envelope-based vaccine against hepatitis C.",

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author = "Jean-Christophe Meunier and Gottwein, {Judith M} and Michael Houghton and Russell, {Rodney S} and Emerson, {Suzanne U} and Jens Bukh and Purcell, {Robert H}",

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T1 - Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus

AU - Meunier, Jean-Christophe

AU - Gottwein, Judith M

AU - Houghton, Michael

AU - Russell, Rodney S

AU - Emerson, Suzanne U

AU - Bukh, Jens

AU - Purcell, Robert H

PY - 2011/10/15

Y1 - 2011/10/15

N2 - We detected cross-reactive neutralizing antibodies (NtAb) against hepatitis C virus (HCV) in chimpanzees vaccinated with HCV-1 (genotype 1a) recombinant E1/E2 envelope glycoproteins. Five vaccinated chimpanzees, protected following HCV-1 challenge, were initially studied using the heterologous H77 (genotype 1a) HCVpp assay. All animals had developed NtAb after the second vaccination; 4 animals had reciprocal titers of =200 at the time of challenge. Using genotypes 1a-6a HCV pseudoparticles (HCVpp) and cell culture-derived HCV (HCVcc) assays, cross-reactive NtAb were detected against 1a, 4a, 5a, and 6a, with limited reactivity against 2a and 3a. Our study provides encouragement for the development of a recombinant envelope-based vaccine against hepatitis C.

AB - We detected cross-reactive neutralizing antibodies (NtAb) against hepatitis C virus (HCV) in chimpanzees vaccinated with HCV-1 (genotype 1a) recombinant E1/E2 envelope glycoproteins. Five vaccinated chimpanzees, protected following HCV-1 challenge, were initially studied using the heterologous H77 (genotype 1a) HCVpp assay. All animals had developed NtAb after the second vaccination; 4 animals had reciprocal titers of =200 at the time of challenge. Using genotypes 1a-6a HCV pseudoparticles (HCVpp) and cell culture-derived HCV (HCVcc) assays, cross-reactive NtAb were detected against 1a, 4a, 5a, and 6a, with limited reactivity against 2a and 3a. Our study provides encouragement for the development of a recombinant envelope-based vaccine against hepatitis C.

KW - Animals

KW - Antibodies, Neutralizing

KW - Cross Reactions

KW - Flow Cytometry

KW - Genotype

KW - Hepacivirus

KW - Hepatitis C, Chronic

KW - Pan troglodytes

KW - Retrospective Studies

KW - Vaccination

KW - Vaccines, Synthetic

KW - Viral Envelope Proteins

KW - Viral Hepatitis Vaccines

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DO - 10.1093/infdis/jir511

M3 - Journal article

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SN - 0022-1899

VL - 204

SP - 1186

EP - 1190

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 8

ER -

Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus

Abstract

Keywords

UN SDGs

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