Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus

Jean-Christophe Meunier; Judith M Gottwein; Michael Houghton; Rodney S Russell; Suzanne U Emerson; Jens Bukh; Robert H Purcell

doi:10.1093/infdis/jir511

Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus

Jean-Christophe Meunier, Judith M Gottwein, Michael Houghton, Rodney S Russell, Suzanne U Emerson, Jens Bukh, Robert H Purcell

LUKKET: Institut for Immunologi og Mikrobiologi

77 Citationer (Scopus)

Abstract

We detected cross-reactive neutralizing antibodies (NtAb) against hepatitis C virus (HCV) in chimpanzees vaccinated with HCV-1 (genotype 1a) recombinant E1/E2 envelope glycoproteins. Five vaccinated chimpanzees, protected following HCV-1 challenge, were initially studied using the heterologous H77 (genotype 1a) HCVpp assay. All animals had developed NtAb after the second vaccination; 4 animals had reciprocal titers of =200 at the time of challenge. Using genotypes 1a-6a HCV pseudoparticles (HCVpp) and cell culture-derived HCV (HCVcc) assays, cross-reactive NtAb were detected against 1a, 4a, 5a, and 6a, with limited reactivity against 2a and 3a. Our study provides encouragement for the development of a recombinant envelope-based vaccine against hepatitis C.

Originalsprog	Engelsk
Tidsskrift	Journal of Infectious Diseases
Vol/bind	204
Udgave nummer	8
Sider (fra-til)	1186-90
Antal sider	5
ISSN	0022-1899
DOI	https://doi.org/10.1093/infdis/jir511
Status	Udgivet - 15 okt. 2011

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10.1093/infdis/jir511

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title = "Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus",

abstract = "We detected cross-reactive neutralizing antibodies (NtAb) against hepatitis C virus (HCV) in chimpanzees vaccinated with HCV-1 (genotype 1a) recombinant E1/E2 envelope glycoproteins. Five vaccinated chimpanzees, protected following HCV-1 challenge, were initially studied using the heterologous H77 (genotype 1a) HCVpp assay. All animals had developed NtAb after the second vaccination; 4 animals had reciprocal titers of =200 at the time of challenge. Using genotypes 1a-6a HCV pseudoparticles (HCVpp) and cell culture-derived HCV (HCVcc) assays, cross-reactive NtAb were detected against 1a, 4a, 5a, and 6a, with limited reactivity against 2a and 3a. Our study provides encouragement for the development of a recombinant envelope-based vaccine against hepatitis C.",

keywords = "Animals, Antibodies, Neutralizing, Cross Reactions, Flow Cytometry, Genotype, Hepacivirus, Hepatitis C, Chronic, Pan troglodytes, Retrospective Studies, Vaccination, Vaccines, Synthetic, Viral Envelope Proteins, Viral Hepatitis Vaccines",

author = "Jean-Christophe Meunier and Gottwein, {Judith M} and Michael Houghton and Russell, {Rodney S} and Emerson, {Suzanne U} and Jens Bukh and Purcell, {Robert H}",

year = "2011",

month = oct,

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doi = "10.1093/infdis/jir511",

language = "English",

volume = "204",

pages = "1186--90",

journal = "Journal of Infectious Diseases",

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TY - JOUR

T1 - Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus

AU - Meunier, Jean-Christophe

AU - Gottwein, Judith M

AU - Houghton, Michael

AU - Russell, Rodney S

AU - Emerson, Suzanne U

AU - Bukh, Jens

AU - Purcell, Robert H

PY - 2011/10/15

Y1 - 2011/10/15

N2 - We detected cross-reactive neutralizing antibodies (NtAb) against hepatitis C virus (HCV) in chimpanzees vaccinated with HCV-1 (genotype 1a) recombinant E1/E2 envelope glycoproteins. Five vaccinated chimpanzees, protected following HCV-1 challenge, were initially studied using the heterologous H77 (genotype 1a) HCVpp assay. All animals had developed NtAb after the second vaccination; 4 animals had reciprocal titers of =200 at the time of challenge. Using genotypes 1a-6a HCV pseudoparticles (HCVpp) and cell culture-derived HCV (HCVcc) assays, cross-reactive NtAb were detected against 1a, 4a, 5a, and 6a, with limited reactivity against 2a and 3a. Our study provides encouragement for the development of a recombinant envelope-based vaccine against hepatitis C.

AB - We detected cross-reactive neutralizing antibodies (NtAb) against hepatitis C virus (HCV) in chimpanzees vaccinated with HCV-1 (genotype 1a) recombinant E1/E2 envelope glycoproteins. Five vaccinated chimpanzees, protected following HCV-1 challenge, were initially studied using the heterologous H77 (genotype 1a) HCVpp assay. All animals had developed NtAb after the second vaccination; 4 animals had reciprocal titers of =200 at the time of challenge. Using genotypes 1a-6a HCV pseudoparticles (HCVpp) and cell culture-derived HCV (HCVcc) assays, cross-reactive NtAb were detected against 1a, 4a, 5a, and 6a, with limited reactivity against 2a and 3a. Our study provides encouragement for the development of a recombinant envelope-based vaccine against hepatitis C.

KW - Animals

KW - Antibodies, Neutralizing

KW - Cross Reactions

KW - Flow Cytometry

KW - Genotype

KW - Hepacivirus

KW - Hepatitis C, Chronic

KW - Pan troglodytes

KW - Retrospective Studies

KW - Vaccination

KW - Vaccines, Synthetic

KW - Viral Envelope Proteins

KW - Viral Hepatitis Vaccines

U2 - 10.1093/infdis/jir511

DO - 10.1093/infdis/jir511

M3 - Journal article

C2 - 21917891

SN - 0022-1899

VL - 204

SP - 1186

EP - 1190

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 8

ER -

Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus

Abstract

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