Abstract
Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (lambda approximately 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the lambda-repressor/ORI operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone.
| Original language | English |
|---|---|
| Journal | FEBS Letters |
| Volume | 235 |
| Issue number | 1-2 |
| Pages (from-to) | 122-4 |
| Number of pages | 3 |
| ISSN | 0014-5793 |
| Publication status | Published - 1 Aug 1988 |
Keywords
- Bacteriophage lambda/analysis
- Base Sequence
- DNA/drug effects
- DNA, Superhelical/drug effects
- Electrophoresis, Polyacrylamide Gel
- Operator Regions, Genetic
- Organometallic Compounds/pharmacology
- Photochemistry
- Plasmids
- Proteins/metabolism
- Repressor Proteins/metabolism
- Ultraviolet Rays
- Uranium/pharmacology
- Uranyl Nitrate/pharmacology