Abstract
The effect on DNA conformation upon binding of the bis-intercalator, N,N'-diacridinyl spermidine is studied by uranyl mediated DNA photocleavage and gel retardation. It is shown that a characteristic A-tract correlated 10 base pair modulation of the uranyl photocleavage of bent (kinetoplast) DNA is suppressed upon binding of the diacridine. Likewise, the anomalous slow gel electrophoretic migration of bent-DNA is abolished by diacridine binding. At higher diacridine/DNA ratios a DNA conformation in which G-residues become hypersensitive to uranyl photocleavage is induced.
| Original language | English |
|---|---|
| Journal | Anti-Cancer Drug Design |
| Volume | 5 |
| Issue number | 1 |
| Pages (from-to) | 105-10 |
| Number of pages | 6 |
| ISSN | 0266-9536 |
| Publication status | Published - Feb 1990 |
Keywords
- DNA/analysis
- DNA Probes/pharmacology
- DNA, Circular/analysis
- DNA, Kinetoplast
- Electrophoresis, Polyacrylamide Gel
- In Vitro Techniques
- Nucleic Acid Conformation/drug effects
- Photochemistry