Uranyl photoprobing of conformational changes in DNA induced by drug binding

N E Møllegaard; Peter E. Nielsen

Uranyl photoprobing of conformational changes in DNA induced by drug binding

Transcription, RNA, and Gene Medicine Program

Abstract

The effect on DNA conformation upon binding of the bis-intercalator, N,N'-diacridinyl spermidine is studied by uranyl mediated DNA photocleavage and gel retardation. It is shown that a characteristic A-tract correlated 10 base pair modulation of the uranyl photocleavage of bent (kinetoplast) DNA is suppressed upon binding of the diacridine. Likewise, the anomalous slow gel electrophoretic migration of bent-DNA is abolished by diacridine binding. At higher diacridine/DNA ratios a DNA conformation in which G-residues become hypersensitive to uranyl photocleavage is induced.

Originalsprog	Engelsk
Tidsskrift	Anti-Cancer Drug Design
Vol/bind	5
Udgave nummer	1
Sider (fra-til)	105-10
Antal sider	6
ISSN	0266-9536
Status	Udgivet - feb. 1990

Citationsformater

@article{20b28417573d4a5199aade9f50a93e73,

title = "Uranyl photoprobing of conformational changes in DNA induced by drug binding",

abstract = "The effect on DNA conformation upon binding of the bis-intercalator, N,N'-diacridinyl spermidine is studied by uranyl mediated DNA photocleavage and gel retardation. It is shown that a characteristic A-tract correlated 10 base pair modulation of the uranyl photocleavage of bent (kinetoplast) DNA is suppressed upon binding of the diacridine. Likewise, the anomalous slow gel electrophoretic migration of bent-DNA is abolished by diacridine binding. At higher diacridine/DNA ratios a DNA conformation in which G-residues become hypersensitive to uranyl photocleavage is induced.",

keywords = "DNA/analysis, DNA Probes/pharmacology, DNA, Circular/analysis, DNA, Kinetoplast, Electrophoresis, Polyacrylamide Gel, In Vitro Techniques, Nucleic Acid Conformation/drug effects, Photochemistry",

author = "M{\o}llegaard, {N E} and Nielsen, {Peter E.}",

year = "1990",

month = feb,

language = "English",

volume = "5",

pages = "105--10",

journal = "Anti-Cancer Drug Design",

issn = "0266-9536",

publisher = "Cognizant Communication Corporation",

number = "1",

}

TY - JOUR

T1 - Uranyl photoprobing of conformational changes in DNA induced by drug binding

AU - Møllegaard, N E

AU - Nielsen, Peter E.

PY - 1990/2

Y1 - 1990/2

N2 - The effect on DNA conformation upon binding of the bis-intercalator, N,N'-diacridinyl spermidine is studied by uranyl mediated DNA photocleavage and gel retardation. It is shown that a characteristic A-tract correlated 10 base pair modulation of the uranyl photocleavage of bent (kinetoplast) DNA is suppressed upon binding of the diacridine. Likewise, the anomalous slow gel electrophoretic migration of bent-DNA is abolished by diacridine binding. At higher diacridine/DNA ratios a DNA conformation in which G-residues become hypersensitive to uranyl photocleavage is induced.

AB - The effect on DNA conformation upon binding of the bis-intercalator, N,N'-diacridinyl spermidine is studied by uranyl mediated DNA photocleavage and gel retardation. It is shown that a characteristic A-tract correlated 10 base pair modulation of the uranyl photocleavage of bent (kinetoplast) DNA is suppressed upon binding of the diacridine. Likewise, the anomalous slow gel electrophoretic migration of bent-DNA is abolished by diacridine binding. At higher diacridine/DNA ratios a DNA conformation in which G-residues become hypersensitive to uranyl photocleavage is induced.

KW - DNA/analysis

KW - DNA Probes/pharmacology

KW - DNA, Circular/analysis

KW - DNA, Kinetoplast

KW - Electrophoresis, Polyacrylamide Gel

KW - In Vitro Techniques

KW - Nucleic Acid Conformation/drug effects

KW - Photochemistry

M3 - Journal article

C2 - 2156513

SN - 0266-9536

VL - 5

SP - 105

EP - 110

JO - Anti-Cancer Drug Design

JF - Anti-Cancer Drug Design

IS - 1

ER -

Uranyl photoprobing of conformational changes in DNA induced by drug binding

Abstract

Fingeraftryk

Citationsformater