Tumor associated antigen specific T-cell populations identified in ex vivo expanded TIL cultures

Niels Junker; Pia Kvistborg; Tania Køllgaard; Per thor Straten; Mads Hald Andersen; Inge Marie Svane

doi:10.1016/j.cellimm.2011.12.004

Tumor associated antigen specific T-cell populations identified in ex vivo expanded TIL cultures

Niels Junker, Pia Kvistborg, Tania Køllgaard, Per thor Straten, Mads Hald Andersen, Inge Marie Svane

19 Citations (Scopus)

Abstract

Ex vivo expanded tumor infiltrating lymphocytes (TILs) from malignant melanoma (MM) and head & neck squamous cell carcinoma (HNSCC) share a similar oligoclonal composition of T effector memory cells, with HLA class I restricted lysis of tumor cell lines. In this study we show that ex vivo expanded TILs from MM and HNSCC demonstrate a heterogeneous composition in frequency and magnitude of tumor associated antigen specific populations by Elispot IFNγ quantitation. TILs from MM and HNSCC shared reactivity towards NY ESO-1, cyclin B1 and Bcl-x derived peptides. Additionally we show that dominating T-cell clones and functionality persists through out expansion among an oligoclonal composition of T-cells. Our findings mirror prior results on the oligoclonal composition of TIL cultures, further indicating a potential for a broader repertoire of specific effector cells recognizing the heterogeneous tumors upon adoptive transfer; increasing the probability of tumor control by minimizing immune evasion by tumor cell escape variants.

Original language	English
Journal	Cellular Immunology
Volume	273
Issue number	1
Pages (from-to)	1-9
Number of pages	9
ISSN	0008-8749
DOIs	https://doi.org/10.1016/j.cellimm.2011.12.004
Publication status	Published - 2012

Access to Document

10.1016/j.cellimm.2011.12.004

Cite this

@article{ae098d837e49418e9e5392925e6de04b,

title = "Tumor associated antigen specific T-cell populations identified in ex vivo expanded TIL cultures",

abstract = "Ex vivo expanded tumor infiltrating lymphocytes (TILs) from malignant melanoma (MM) and head & neck squamous cell carcinoma (HNSCC) share a similar oligoclonal composition of T effector memory cells, with HLA class I restricted lysis of tumor cell lines. In this study we show that ex vivo expanded TILs from MM and HNSCC demonstrate a heterogeneous composition in frequency and magnitude of tumor associated antigen specific populations by Elispot IFNγ quantitation. TILs from MM and HNSCC shared reactivity towards NY ESO-1, cyclin B1 and Bcl-x derived peptides. Additionally we show that dominating T-cell clones and functionality persists through out expansion among an oligoclonal composition of T-cells. Our findings mirror prior results on the oligoclonal composition of TIL cultures, further indicating a potential for a broader repertoire of specific effector cells recognizing the heterogeneous tumors upon adoptive transfer; increasing the probability of tumor control by minimizing immune evasion by tumor cell escape variants.",

author = "Niels Junker and Pia Kvistborg and Tania K{\o}llgaard and Straten, {Per thor} and Andersen, {Mads Hald} and Svane, {Inge Marie}",

year = "2012",

doi = "10.1016/j.cellimm.2011.12.004",

language = "English",

volume = "273",

pages = "1--9",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press",

number = "1",

}

TY - JOUR

T1 - Tumor associated antigen specific T-cell populations identified in ex vivo expanded TIL cultures

AU - Junker, Niels

AU - Kvistborg, Pia

AU - Køllgaard, Tania

AU - Straten, Per thor

AU - Andersen, Mads Hald

AU - Svane, Inge Marie

PY - 2012

Y1 - 2012

N2 - Ex vivo expanded tumor infiltrating lymphocytes (TILs) from malignant melanoma (MM) and head & neck squamous cell carcinoma (HNSCC) share a similar oligoclonal composition of T effector memory cells, with HLA class I restricted lysis of tumor cell lines. In this study we show that ex vivo expanded TILs from MM and HNSCC demonstrate a heterogeneous composition in frequency and magnitude of tumor associated antigen specific populations by Elispot IFNγ quantitation. TILs from MM and HNSCC shared reactivity towards NY ESO-1, cyclin B1 and Bcl-x derived peptides. Additionally we show that dominating T-cell clones and functionality persists through out expansion among an oligoclonal composition of T-cells. Our findings mirror prior results on the oligoclonal composition of TIL cultures, further indicating a potential for a broader repertoire of specific effector cells recognizing the heterogeneous tumors upon adoptive transfer; increasing the probability of tumor control by minimizing immune evasion by tumor cell escape variants.

AB - Ex vivo expanded tumor infiltrating lymphocytes (TILs) from malignant melanoma (MM) and head & neck squamous cell carcinoma (HNSCC) share a similar oligoclonal composition of T effector memory cells, with HLA class I restricted lysis of tumor cell lines. In this study we show that ex vivo expanded TILs from MM and HNSCC demonstrate a heterogeneous composition in frequency and magnitude of tumor associated antigen specific populations by Elispot IFNγ quantitation. TILs from MM and HNSCC shared reactivity towards NY ESO-1, cyclin B1 and Bcl-x derived peptides. Additionally we show that dominating T-cell clones and functionality persists through out expansion among an oligoclonal composition of T-cells. Our findings mirror prior results on the oligoclonal composition of TIL cultures, further indicating a potential for a broader repertoire of specific effector cells recognizing the heterogeneous tumors upon adoptive transfer; increasing the probability of tumor control by minimizing immune evasion by tumor cell escape variants.

U2 - 10.1016/j.cellimm.2011.12.004

DO - 10.1016/j.cellimm.2011.12.004

M3 - Journal article

C2 - 22230732

SN - 0008-8749

VL - 273

SP - 1

EP - 9

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -

Tumor associated antigen specific T-cell populations identified in ex vivo expanded TIL cultures

Abstract

Access to Document

Fingerprint

Cite this