Tumor associated antigen specific T-cell populations identified in ex vivo expanded TIL cultures

TY - JOUR

T1 - Tumor associated antigen specific T-cell populations identified in ex vivo expanded TIL cultures

AU - Junker, Niels

AU - Kvistborg, Pia

AU - Køllgaard, Tania

AU - Straten, Per thor

AU - Andersen, Mads Hald

AU - Svane, Inge Marie

N1 - Copyright © 2011 Elsevier Inc. All rights reserved.

PY - 2012

Y1 - 2012

N2 - Ex vivo expanded tumor infiltrating lymphocytes (TILs) from malignant melanoma (MM) and head & neck squamous cell carcinoma (HNSCC) share a similar oligoclonal composition of T effector memory cells, with HLA class I restricted lysis of tumor cell lines. In this study we show that ex vivo expanded TILs from MM and HNSCC demonstrate a heterogeneous composition in frequency and magnitude of tumor associated antigen specific populations by Elispot IFNγ quantitation. TILs from MM and HNSCC shared reactivity towards NY ESO-1, cyclin B1 and Bcl-x derived peptides. Additionally we show that dominating T-cell clones and functionality persists through out expansion among an oligoclonal composition of T-cells. Our findings mirror prior results on the oligoclonal composition of TIL cultures, further indicating a potential for a broader repertoire of specific effector cells recognizing the heterogeneous tumors upon adoptive transfer; increasing the probability of tumor control by minimizing immune evasion by tumor cell escape variants.

AB - Ex vivo expanded tumor infiltrating lymphocytes (TILs) from malignant melanoma (MM) and head & neck squamous cell carcinoma (HNSCC) share a similar oligoclonal composition of T effector memory cells, with HLA class I restricted lysis of tumor cell lines. In this study we show that ex vivo expanded TILs from MM and HNSCC demonstrate a heterogeneous composition in frequency and magnitude of tumor associated antigen specific populations by Elispot IFNγ quantitation. TILs from MM and HNSCC shared reactivity towards NY ESO-1, cyclin B1 and Bcl-x derived peptides. Additionally we show that dominating T-cell clones and functionality persists through out expansion among an oligoclonal composition of T-cells. Our findings mirror prior results on the oligoclonal composition of TIL cultures, further indicating a potential for a broader repertoire of specific effector cells recognizing the heterogeneous tumors upon adoptive transfer; increasing the probability of tumor control by minimizing immune evasion by tumor cell escape variants.

U2 - 10.1016/j.cellimm.2011.12.004

DO - 10.1016/j.cellimm.2011.12.004

M3 - Journal article

C2 - 22230732

SN - 0008-8749

VL - 273

SP - 1

EP - 9

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -