Transfection of tumor-infiltrating T cells with mRNA encoding CXCR2

TY - CHAP

T1 - Transfection of tumor-infiltrating T cells with mRNA encoding CXCR2

AU - Idorn, Manja

AU - thor Straten, Eivind Per

AU - Svane, Inge Marie

AU - Met, Özcan

PY - 2016

Y1 - 2016

N2 - Adoptive T-cell therapy based on the infusion of patient’s own immune cells after ex vivo culturing is among the most potent forms of personalized treatment among recent clinical developments for the treatment of cancer. However, despite high rates of successful initial clinical responses, only about 20 % of patients with metastatic melanoma treated with tumor-infiltrating lymphocytes (TILs) enter complete and long-term regression, with the majority either relapsing after initial partial regression or not benefiting at all. Previous studies have shown a positive correlation between the number infused T cells migrating to the tumor and the clinical response, but also that only a small fraction of adoptively transferred Tcells reach the tumor site. In this chapter, we describe a protocol for transfection of TILs with mRNA encoding the chemokine receptor CXCR2 transiently redirecting and improving TILs migration toward tumor-secreted chemokines in vitro.

AB - Adoptive T-cell therapy based on the infusion of patient’s own immune cells after ex vivo culturing is among the most potent forms of personalized treatment among recent clinical developments for the treatment of cancer. However, despite high rates of successful initial clinical responses, only about 20 % of patients with metastatic melanoma treated with tumor-infiltrating lymphocytes (TILs) enter complete and long-term regression, with the majority either relapsing after initial partial regression or not benefiting at all. Previous studies have shown a positive correlation between the number infused T cells migrating to the tumor and the clinical response, but also that only a small fraction of adoptively transferred Tcells reach the tumor site. In this chapter, we describe a protocol for transfection of TILs with mRNA encoding the chemokine receptor CXCR2 transiently redirecting and improving TILs migration toward tumor-secreted chemokines in vitro.

KW - Cancer immunotherapy

KW - Chemokine receptor

KW - mRNA transfection

KW - Tumor homing

KW - Tumor-infiltrating lymphocytes (TILs)

U2 - 10.1007/978-1-4939-3625-0_17

DO - 10.1007/978-1-4939-3625-0_17

M3 - Book chapter

C2 - 27236805

AN - SCOPUS:84990194133

SN - 978-1-4939-3623-6

VL - 1428

T3 - Methods in Molecular Biology

SP - 261

EP - 276

BT - Synthetic mRNA

A2 - Rhoads, Robert E.

PB - Springer

ER -