Abstract
Mixed pyrimidine-purine peptide nucleic acids (PNAs) composed of thymines and guanines are shown to form a PNA(2)-DNA triplex with Watson-Crick complementary adenine-cytosine oligonucleotides and to bind complementary adenine-cytosine targets in double stranded DNA by helix invasion. These results for the first time demonstrate binding of an unmodified PNA oligomer to a mixed pyrimidine-purine target in double stranded DNA and illustrate a novel binding mode of PNA.
| Original language | English |
|---|---|
| Journal | Bioorganic & Medicinal Chemistry |
| Volume | 9 |
| Issue number | 9 |
| Pages (from-to) | 2429-34 |
| Number of pages | 6 |
| ISSN | 0968-0896 |
| Publication status | Published - Sept 2001 |
Keywords
- Base Composition
- Base Pairing
- DNA/chemistry
- Dinucleoside Phosphates/metabolism
- Guanine
- Hydrogen Bonding
- Nucleic Acid Denaturation
- Peptide Nucleic Acids/chemistry
- Temperature
- Thymine