Strand displacement recognition of mixed adenine-cytosine sequences in double stranded DNA by thymine-guanine PNA (peptide nucleic acid)

Peter E. Nielsen

Strand displacement recognition of mixed adenine-cytosine sequences in double stranded DNA by thymine-guanine PNA (peptide nucleic acid)

Transcription, RNA, and Gene Medicine Program

Abstract

Mixed pyrimidine-purine peptide nucleic acids (PNAs) composed of thymines and guanines are shown to form a PNA(2)-DNA triplex with Watson-Crick complementary adenine-cytosine oligonucleotides and to bind complementary adenine-cytosine targets in double stranded DNA by helix invasion. These results for the first time demonstrate binding of an unmodified PNA oligomer to a mixed pyrimidine-purine target in double stranded DNA and illustrate a novel binding mode of PNA.

Originalsprog	Engelsk
Tidsskrift	Bioorganic & Medicinal Chemistry
Vol/bind	9
Udgave nummer	9
Sider (fra-til)	2429-34
Antal sider	6
ISSN	0968-0896
Status	Udgivet - sep. 2001

Citationsformater

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title = "Strand displacement recognition of mixed adenine-cytosine sequences in double stranded DNA by thymine-guanine PNA (peptide nucleic acid)",

abstract = "Mixed pyrimidine-purine peptide nucleic acids (PNAs) composed of thymines and guanines are shown to form a PNA(2)-DNA triplex with Watson-Crick complementary adenine-cytosine oligonucleotides and to bind complementary adenine-cytosine targets in double stranded DNA by helix invasion. These results for the first time demonstrate binding of an unmodified PNA oligomer to a mixed pyrimidine-purine target in double stranded DNA and illustrate a novel binding mode of PNA.",

keywords = "Base Composition, Base Pairing, DNA/chemistry, Dinucleoside Phosphates/metabolism, Guanine, Hydrogen Bonding, Nucleic Acid Denaturation, Peptide Nucleic Acids/chemistry, Temperature, Thymine",

author = "Nielsen, {Peter E.}",

year = "2001",

month = sep,

language = "English",

volume = "9",

pages = "2429--34",

journal = "Bioorganic & Medicinal Chemistry",

issn = "0968-0896",

publisher = "Pergamon Press",

number = "9",

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TY - JOUR

T1 - Strand displacement recognition of mixed adenine-cytosine sequences in double stranded DNA by thymine-guanine PNA (peptide nucleic acid)

AU - Nielsen, Peter E.

PY - 2001/9

Y1 - 2001/9

N2 - Mixed pyrimidine-purine peptide nucleic acids (PNAs) composed of thymines and guanines are shown to form a PNA(2)-DNA triplex with Watson-Crick complementary adenine-cytosine oligonucleotides and to bind complementary adenine-cytosine targets in double stranded DNA by helix invasion. These results for the first time demonstrate binding of an unmodified PNA oligomer to a mixed pyrimidine-purine target in double stranded DNA and illustrate a novel binding mode of PNA.

AB - Mixed pyrimidine-purine peptide nucleic acids (PNAs) composed of thymines and guanines are shown to form a PNA(2)-DNA triplex with Watson-Crick complementary adenine-cytosine oligonucleotides and to bind complementary adenine-cytosine targets in double stranded DNA by helix invasion. These results for the first time demonstrate binding of an unmodified PNA oligomer to a mixed pyrimidine-purine target in double stranded DNA and illustrate a novel binding mode of PNA.

KW - Base Composition

KW - Base Pairing

KW - DNA/chemistry

KW - Dinucleoside Phosphates/metabolism

KW - Guanine

KW - Hydrogen Bonding

KW - Nucleic Acid Denaturation

KW - Peptide Nucleic Acids/chemistry

KW - Temperature

KW - Thymine

M3 - Journal article

C2 - 11553484

SN - 0968-0896

VL - 9

SP - 2429

EP - 2434

JO - Bioorganic & Medicinal Chemistry

JF - Bioorganic & Medicinal Chemistry

IS - 9

ER -

Strand displacement recognition of mixed adenine-cytosine sequences in double stranded DNA by thymine-guanine PNA (peptide nucleic acid)

Abstract

Fingeraftryk

Citationsformater