Repeated extraction of DNA from FTA cards

Michael Stangegaard; Laura Ferrero; Claus Børsting; Rune Frank-Hansen; Anders Johannes Hansen; Niels Morling

doi:10.1016/j.fsigss.2011.09.035

Repeated extraction of DNA from FTA cards

Michael Stangegaard, Laura Ferrero, Claus Børsting, Rune Frank-Hansen, Anders Johannes Hansen, Niels Morling

4 Citations (Scopus)

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Abstract

Extraction of DNA using magnetic bead based techniques on automated DNA extraction instruments provides a fast, reliable and reproducible method for DNA extraction from various matrices. However, the yield of extracted DNA from FTA-cards is typically low. Here, we demonstrate that it is possible to repeatedly extract DNA from the processed FTA-disk. The method increases the yield from the nanogram range to the microgram range.

Original language	English
Journal	Forensic Science International: Genetics. Supplement Series
Volume	3
Issue number	1
Pages (from-to)	e345-e346
Number of pages	2
ISSN	1875-1768
DOIs	https://doi.org/10.1016/j.fsigss.2011.09.035
Publication status	Published - Dec 2011
Event	24th International ISFG Congress: World Congress of the International Society for Forensic Genetics - University of Vienna, Wien, Austria Duration: 29 Aug 2011 → 3 Sept 2011 Conference number: 24

Conference

Conference	24th International ISFG Congress
Number	24
Location	University of Vienna
Country/Territory	Austria
City	Wien
Period	29/08/2011 → 03/09/2011

Access to Document

10.1016/j.fsigss.2011.09.035

Cite this

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title = "Repeated extraction of DNA from FTA cards",

abstract = "Extraction of DNA using magnetic bead based techniques on automated DNA extraction instruments provides a fast, reliable and reproducible method for DNA extraction from various matrices. However, the yield of extracted DNA from FTA-cards is typically low. Here, we demonstrate that it is possible to repeatedly extract DNA from the processed FTA-disk. The method increases the yield from the nanogram range to the microgram range.",

author = "Michael Stangegaard and Laura Ferrero and Claus B{\o}rsting and Rune Frank-Hansen and Hansen, {Anders Johannes} and Niels Morling",

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doi = "10.1016/j.fsigss.2011.09.035",

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journal = "Forensic Science International: Genetics. Supplement Series",

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publisher = "Elsevier Ireland Ltd",

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note = "24th International ISFG Congress ; Conference date: 29-08-2011 Through 03-09-2011",

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T1 - Repeated extraction of DNA from FTA cards

AU - Stangegaard, Michael

AU - Ferrero, Laura

AU - Børsting, Claus

AU - Frank-Hansen, Rune

AU - Hansen, Anders Johannes

AU - Morling, Niels

N1 - Conference code: 24

PY - 2011/12

Y1 - 2011/12

N2 - Extraction of DNA using magnetic bead based techniques on automated DNA extraction instruments provides a fast, reliable and reproducible method for DNA extraction from various matrices. However, the yield of extracted DNA from FTA-cards is typically low. Here, we demonstrate that it is possible to repeatedly extract DNA from the processed FTA-disk. The method increases the yield from the nanogram range to the microgram range.

AB - Extraction of DNA using magnetic bead based techniques on automated DNA extraction instruments provides a fast, reliable and reproducible method for DNA extraction from various matrices. However, the yield of extracted DNA from FTA-cards is typically low. Here, we demonstrate that it is possible to repeatedly extract DNA from the processed FTA-disk. The method increases the yield from the nanogram range to the microgram range.

U2 - 10.1016/j.fsigss.2011.09.035

DO - 10.1016/j.fsigss.2011.09.035

M3 - Conference article

SN - 1875-1768

VL - 3

SP - e345-e346

JO - Forensic Science International: Genetics. Supplement Series

JF - Forensic Science International: Genetics. Supplement Series

IS - 1

T2 - 24th International ISFG Congress

Y2 - 29 August 2011 through 3 September 2011

ER -