Repeated extraction of DNA from FTA cards

Michael Stangegaard; Laura Ferrero; Claus Børsting; Rune Frank-Hansen; Anders Johannes Hansen; Niels Morling

doi:10.1016/j.fsigss.2011.09.035

Repeated extraction of DNA from FTA cards

Michael Stangegaard, Laura Ferrero, Claus Børsting, Rune Frank-Hansen, Anders Johannes Hansen, Niels Morling

4 Citationer (Scopus)

4580 Downloads (Pure)

Abstract

Extraction of DNA using magnetic bead based techniques on automated DNA extraction instruments provides a fast, reliable and reproducible method for DNA extraction from various matrices. However, the yield of extracted DNA from FTA-cards is typically low. Here, we demonstrate that it is possible to repeatedly extract DNA from the processed FTA-disk. The method increases the yield from the nanogram range to the microgram range.

Originalsprog	Engelsk
Tidsskrift	Forensic Science International: Genetics. Supplement Series
Vol/bind	3
Udgave nummer	1
Sider (fra-til)	e345-e346
Antal sider	2
ISSN	1875-1768
DOI	https://doi.org/10.1016/j.fsigss.2011.09.035
Status	Udgivet - dec. 2011
Begivenhed	24th International ISFG Congress: World Congress of the International Society for Forensic Genetics - University of Vienna, Wien, Østrig Varighed: 29 aug. 2011 → 3 sep. 2011 Konferencens nummer: 24

Konference

Konference	24th International ISFG Congress
Nummer	24
Lokation	University of Vienna
Land/Område	Østrig
By	Wien
Periode	29/08/2011 → 03/09/2011

Adgang til dokumentet

10.1016/j.fsigss.2011.09.035

Citationsformater

@inproceedings{32706584484a4848ad14f008df58f5d2,

title = "Repeated extraction of DNA from FTA cards",

abstract = "Extraction of DNA using magnetic bead based techniques on automated DNA extraction instruments provides a fast, reliable and reproducible method for DNA extraction from various matrices. However, the yield of extracted DNA from FTA-cards is typically low. Here, we demonstrate that it is possible to repeatedly extract DNA from the processed FTA-disk. The method increases the yield from the nanogram range to the microgram range.",

author = "Michael Stangegaard and Laura Ferrero and Claus B{\o}rsting and Rune Frank-Hansen and Hansen, {Anders Johannes} and Niels Morling",

year = "2011",

month = dec,

doi = "10.1016/j.fsigss.2011.09.035",

language = "English",

volume = "3",

pages = "e345--e346",

journal = "Forensic Science International: Genetics. Supplement Series",

issn = "1875-1768",

publisher = "Elsevier Ireland Ltd",

number = "1",

note = "24th International ISFG Congress ; Conference date: 29-08-2011 Through 03-09-2011",

}

TY - GEN

T1 - Repeated extraction of DNA from FTA cards

AU - Stangegaard, Michael

AU - Ferrero, Laura

AU - Børsting, Claus

AU - Frank-Hansen, Rune

AU - Hansen, Anders Johannes

AU - Morling, Niels

N1 - Conference code: 24

PY - 2011/12

Y1 - 2011/12

N2 - Extraction of DNA using magnetic bead based techniques on automated DNA extraction instruments provides a fast, reliable and reproducible method for DNA extraction from various matrices. However, the yield of extracted DNA from FTA-cards is typically low. Here, we demonstrate that it is possible to repeatedly extract DNA from the processed FTA-disk. The method increases the yield from the nanogram range to the microgram range.

AB - Extraction of DNA using magnetic bead based techniques on automated DNA extraction instruments provides a fast, reliable and reproducible method for DNA extraction from various matrices. However, the yield of extracted DNA from FTA-cards is typically low. Here, we demonstrate that it is possible to repeatedly extract DNA from the processed FTA-disk. The method increases the yield from the nanogram range to the microgram range.

U2 - 10.1016/j.fsigss.2011.09.035

DO - 10.1016/j.fsigss.2011.09.035

M3 - Conference article

SN - 1875-1768

VL - 3

SP - e345-e346

JO - Forensic Science International: Genetics. Supplement Series

JF - Forensic Science International: Genetics. Supplement Series

IS - 1

T2 - 24th International ISFG Congress

Y2 - 29 August 2011 through 3 September 2011

ER -