RBP2 belongs to a family of demethylases, specific for tri-and dimethylated lysine 4 on histone 3.

TY - JOUR

T1 - RBP2 belongs to a family of demethylases, specific for tri-and dimethylated lysine 4 on histone 3.

AU - Christensen, Jesper

AU - Agger, Karl

AU - Cloos, Paul A C

AU - Pasini, Diego

AU - Rose, Simon

AU - Sennels, Lau

AU - Rappsilber, Juri

AU - Hansen, Klaus

AU - Salcini, Anna Elisabetta

AU - Helin, Kristian

N1 - Keywords: Amino Acid Sequence; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Carrier Proteins; DNA-Binding Proteins; Drosophila melanogaster; Embryonic Stem Cells; Gene Deletion; Genes, Homeobox; Histones; Humans; Intracellular Signaling Peptides and Proteins; Lysine; Methylation; Mice; Molecular Sequence Data; Nuclear Proteins; Oxidoreductases, N-Demethylating; Phylogeny; Protein Structure, Tertiary; Proteins; Repressor Proteins; Schizosaccharomyces; Sequence Alignment; Tumor Suppressor Proteins

PY - 2007

Y1 - 2007

N2 - Methylation of histones has been regarded as a stable modification defining the epigenetic program of the cell, which regulates chromatin structure and transcription. However, the recent discovery of histone demethylases has challenged the stable nature of histone methylation. Here we demonstrate that the JARID1 proteins RBP2, PLU1, and SMCX are histone demethylases specific for di- and trimethylated histone 3 lysine 4 (H3K4). Consistent with a role for the JARID1 Drosophila homolog Lid in regulating expression of homeotic genes during development, we show that RBP2 is displaced from Hox genes during embryonic stem (ES) cell differentiation correlating with an increase of their H3K4me3 levels and expression. Furthermore, we show that mutation or RNAi depletion of the C. elegans JARID1 homolog rbr-2 leads to increased levels of H3K4me3 during larval development and defects in vulva formation. Taken together, these results suggest that H3K4me3/me2 demethylation regulated by the JARID1 family plays an important role during development.

AB - Methylation of histones has been regarded as a stable modification defining the epigenetic program of the cell, which regulates chromatin structure and transcription. However, the recent discovery of histone demethylases has challenged the stable nature of histone methylation. Here we demonstrate that the JARID1 proteins RBP2, PLU1, and SMCX are histone demethylases specific for di- and trimethylated histone 3 lysine 4 (H3K4). Consistent with a role for the JARID1 Drosophila homolog Lid in regulating expression of homeotic genes during development, we show that RBP2 is displaced from Hox genes during embryonic stem (ES) cell differentiation correlating with an increase of their H3K4me3 levels and expression. Furthermore, we show that mutation or RNAi depletion of the C. elegans JARID1 homolog rbr-2 leads to increased levels of H3K4me3 during larval development and defects in vulva formation. Taken together, these results suggest that H3K4me3/me2 demethylation regulated by the JARID1 family plays an important role during development.

U2 - 10.1016/j.cell.2007.02.003

DO - 10.1016/j.cell.2007.02.003

M3 - Journal article

C2 - 17320161

SN - 0092-8674

VL - 128

SP - 1063

EP - 1076

JO - Cell

JF - Cell

IS - 6

ER -