Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

Marc Stegger; Jodi A. Lindsay; Arshnee Moodley; Robert Skov; Els M. Broens; Luca Guardabassi

doi:10.1128/JCM.01970-10

Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

Marc Stegger, Jodi A. Lindsay, Arshnee Moodley, Robert Skov, Els M. Broens, Luca Guardabassi

85 Citations (Scopus)

Abstract

A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillinresistant S. aureus (MRSA) CC398 in hospitals and on farms.

Original language	English
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	2
Pages (from-to)	732-734
Number of pages	3
ISSN	0095-1137
DOIs	https://doi.org/10.1128/JCM.01970-10
Publication status	Published - Feb 2011

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http://jcm.asm.org/cgi/content/full/49/2/732?view=long&pmid=21123532

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title = "Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1",

abstract = "A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillinresistant S. aureus (MRSA) CC398 in hospitals and on farms.",

author = "Marc Stegger and Lindsay, {Jodi A.} and Arshnee Moodley and Robert Skov and Broens, {Els M.} and Luca Guardabassi",

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AU - Stegger, Marc

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AU - Skov, Robert

AU - Broens, Els M.

AU - Guardabassi, Luca

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AB - A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillinresistant S. aureus (MRSA) CC398 in hospitals and on farms.

U2 - 10.1128/JCM.01970-10

DO - 10.1128/JCM.01970-10

M3 - Journal article

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

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Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

Abstract

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