Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

Marc Stegger; Jodi A. Lindsay; Arshnee Moodley; Robert Skov; Els M. Broens; Luca Guardabassi

doi:10.1128/JCM.01970-10

Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

Marc Stegger, Jodi A. Lindsay, Arshnee Moodley, Robert Skov, Els M. Broens, Luca Guardabassi

85 Citationer (Scopus)

Abstract

A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillinresistant S. aureus (MRSA) CC398 in hospitals and on farms.

Originalsprog	Engelsk
Tidsskrift	Journal of Clinical Microbiology
Vol/bind	49
Udgave nummer	2
Sider (fra-til)	732-734
Antal sider	3
ISSN	0095-1137
DOI	https://doi.org/10.1128/JCM.01970-10
Status	Udgivet - feb. 2011

Adgang til dokumentet

10.1128/JCM.01970-10

http://jcm.asm.org/cgi/content/full/49/2/732?view=long&pmid=21123532

Citationsformater

@article{1aae4f11f3234e1da38d9973fa48c64e,

title = "Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1",

abstract = "A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillinresistant S. aureus (MRSA) CC398 in hospitals and on farms.",

author = "Marc Stegger and Lindsay, {Jodi A.} and Arshnee Moodley and Robert Skov and Broens, {Els M.} and Luca Guardabassi",

year = "2011",

month = feb,

doi = "10.1128/JCM.01970-10",

language = "English",

volume = "49",

pages = "732--734",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "2",

}

TY - JOUR

T1 - Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

AU - Stegger, Marc

AU - Lindsay, Jodi A.

AU - Moodley, Arshnee

AU - Skov, Robert

AU - Broens, Els M.

AU - Guardabassi, Luca

PY - 2011/2

Y1 - 2011/2

N2 - A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillinresistant S. aureus (MRSA) CC398 in hospitals and on farms.

AB - A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillinresistant S. aureus (MRSA) CC398 in hospitals and on farms.

U2 - 10.1128/JCM.01970-10

DO - 10.1128/JCM.01970-10

M3 - Journal article

C2 - 21123532

SN - 0095-1137

VL - 49

SP - 732

EP - 734

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 2

ER -

Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater