Rad52 and Rad59 exhibit both overlapping and distinct functions

Q Feng; Louis Düring; A. A. de Mayolo; G. Lettier; M. Lisby; N. Erdeniz; U. H. Mortensen; R. Rothstein

doi:10.1016/j.dnarep.2006.08.007

Rad52 and Rad59 exhibit both overlapping and distinct functions

Q Feng, Louis Düring, A. A. de Mayolo, G. Lettier, M. Lisby, N. Erdeniz, U. H. Mortensen, R. Rothstein

Functional Genomics

31 Citations (Scopus)

Abstract

Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.

Original language	English
Journal	DNA Repair
Volume	6
Issue number	1
Pages (from-to)	27-37
ISSN	1568-7864
DOIs	https://doi.org/10.1016/j.dnarep.2006.08.007
Publication status	Published - 2007

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10.1016/j.dnarep.2006.08.007

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title = "Rad52 and Rad59 exhibit both overlapping and distinct functions",

abstract = "Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.",

author = "Q Feng and Louis D{\"u}ring and Mayolo, {A. A. de} and G. Lettier and M. Lisby and N. Erdeniz and Mortensen, {U. H.} and R. Rothstein",

note = "Keywords: Rad52; Rad59; Recombination foci; Homologous recombination; Saccharomyces cerevisiae",

year = "2007",

doi = "10.1016/j.dnarep.2006.08.007",

language = "English",

volume = "6",

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TY - JOUR

T1 - Rad52 and Rad59 exhibit both overlapping and distinct functions

AU - Feng, Q

AU - Düring, Louis

AU - Mayolo, A. A. de

AU - Lettier, G.

AU - Lisby, M.

AU - Erdeniz, N.

AU - Mortensen, U. H.

AU - Rothstein, R.

N1 - Keywords: Rad52; Rad59; Recombination foci; Homologous recombination; Saccharomyces cerevisiae

PY - 2007

Y1 - 2007

N2 - Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.

AB - Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.

U2 - 10.1016/j.dnarep.2006.08.007

DO - 10.1016/j.dnarep.2006.08.007

M3 - Journal article

SN - 1568-7864

VL - 6

SP - 27

EP - 37

JO - DNA Repair

JF - DNA Repair

IS - 1

ER -

Rad52 and Rad59 exhibit both overlapping and distinct functions

Abstract

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