TY - JOUR
T1 - Rad52 and Rad59 exhibit both overlapping and distinct functions
AU - Feng, Q
AU - Düring, Louis
AU - Mayolo, A. A. de
AU - Lettier, G.
AU - Lisby, M.
AU - Erdeniz, N.
AU - Mortensen, U. H.
AU - Rothstein, R.
N1 - Keywords: Rad52; Rad59; Recombination foci; Homologous recombination; Saccharomyces cerevisiae
PY - 2007
Y1 - 2007
N2 - Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.
AB - Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.
U2 - 10.1016/j.dnarep.2006.08.007
DO - 10.1016/j.dnarep.2006.08.007
M3 - Journal article
SN - 1568-7864
VL - 6
SP - 27
EP - 37
JO - DNA Repair
JF - DNA Repair
IS - 1
ER -