Quantifying protein-protein interactions in the ubiquitin pathway by surface plasmon resonance.

Rasmus Hartmann-Petersen; Colin Gordon

doi:10.1016/S0076-6879(05)99011-3

Quantifying protein-protein interactions in the ubiquitin pathway by surface plasmon resonance.

Rasmus Hartmann-Petersen, Colin Gordon

Biomolecular Sciences

11 Citations (Scopus)

Abstract

The commercial availability of instruments, such as Biacore, that are capable of monitoring surface plasmon resonance (SPR) has greatly simplified the quantification of protein-protein interactions. Already, this technique has been used for some studies of the ubiquitin-proteasome system. Here we discuss some of the problems and pitfalls that researchers should be aware of when using SPR analyses for studies of the ubiquitin-proteasome system.

Original language	English
Book series	Methods in Enzymology
Volume	399
Pages (from-to)	164-77
Number of pages	13
ISSN	0076-6879
DOIs	https://doi.org/10.1016/S0076-6879(05)99011-3
Publication status	Published - 2005

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@article{4d0636a095f211dd86a6000ea68e967b,

title = "Quantifying protein-protein interactions in the ubiquitin pathway by surface plasmon resonance.",

abstract = "The commercial availability of instruments, such as Biacore, that are capable of monitoring surface plasmon resonance (SPR) has greatly simplified the quantification of protein-protein interactions. Already, this technique has been used for some studies of the ubiquitin-proteasome system. Here we discuss some of the problems and pitfalls that researchers should be aware of when using SPR analyses for studies of the ubiquitin-proteasome system.",

author = "Rasmus Hartmann-Petersen and Colin Gordon",

note = "Keywords: Protein Binding; Proteins; Surface Plasmon Resonance; Ubiquitin",

year = "2005",

doi = "10.1016/S0076-6879(05)99011-3",

language = "English",

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T1 - Quantifying protein-protein interactions in the ubiquitin pathway by surface plasmon resonance.

AU - Hartmann-Petersen, Rasmus

AU - Gordon, Colin

N1 - Keywords: Protein Binding; Proteins; Surface Plasmon Resonance; Ubiquitin

PY - 2005

Y1 - 2005

N2 - The commercial availability of instruments, such as Biacore, that are capable of monitoring surface plasmon resonance (SPR) has greatly simplified the quantification of protein-protein interactions. Already, this technique has been used for some studies of the ubiquitin-proteasome system. Here we discuss some of the problems and pitfalls that researchers should be aware of when using SPR analyses for studies of the ubiquitin-proteasome system.

AB - The commercial availability of instruments, such as Biacore, that are capable of monitoring surface plasmon resonance (SPR) has greatly simplified the quantification of protein-protein interactions. Already, this technique has been used for some studies of the ubiquitin-proteasome system. Here we discuss some of the problems and pitfalls that researchers should be aware of when using SPR analyses for studies of the ubiquitin-proteasome system.

U2 - 10.1016/S0076-6879(05)99011-3

DO - 10.1016/S0076-6879(05)99011-3

M3 - Journal article

C2 - 16338355

SN - 0076-6879

VL - 399

SP - 164

EP - 177

JO - Methods in Enzymology

JF - Methods in Enzymology

ER -

Quantifying protein-protein interactions in the ubiquitin pathway by surface plasmon resonance.

Abstract

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