TY - JOUR
T1 - Quantifying protein-protein interactions in the ubiquitin pathway by surface plasmon resonance.
AU - Hartmann-Petersen, Rasmus
AU - Gordon, Colin
N1 - Keywords: Protein Binding; Proteins; Surface Plasmon Resonance; Ubiquitin
PY - 2005
Y1 - 2005
N2 - The commercial availability of instruments, such as Biacore, that are capable of monitoring surface plasmon resonance (SPR) has greatly simplified the quantification of protein-protein interactions. Already, this technique has been used for some studies of the ubiquitin-proteasome system. Here we discuss some of the problems and pitfalls that researchers should be aware of when using SPR analyses for studies of the ubiquitin-proteasome system.
AB - The commercial availability of instruments, such as Biacore, that are capable of monitoring surface plasmon resonance (SPR) has greatly simplified the quantification of protein-protein interactions. Already, this technique has been used for some studies of the ubiquitin-proteasome system. Here we discuss some of the problems and pitfalls that researchers should be aware of when using SPR analyses for studies of the ubiquitin-proteasome system.
U2 - 10.1016/S0076-6879(05)99011-3
DO - 10.1016/S0076-6879(05)99011-3
M3 - Journal article
C2 - 16338355
SN - 0076-6879
VL - 399
SP - 164
EP - 177
JO - Methods in Enzymology
JF - Methods in Enzymology
ER -