Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts

Line Maria Irlund Pedersen; Michael Stangegaard; Helle Smidt Mogensen; Niels Morling

doi:10.1016/j.fsigss.2011.09.036

Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts

Line Maria Irlund Pedersen, Michael Stangegaard, Helle Smidt Mogensen, Niels Morling

2 Citations (Scopus)

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Abstract

Capillary electrophoresis of amplified DNA isolated from trace evidence samples occasionally results in inadequate STR-profiles due to artifacts caused by e.g. primers and dNTPs. Removal of artifacts by purification and subsequent concentration of the PCR products may increase the sensitivity and the quality of the DNA profiles without re-amplification of the sample. We have validated and implemented an automated method to purify and 2-fold concentrate PCR products resulting in allelic peaks with higher intensity (a median height across all loci from 130 to 404 RFU), fewer allelic dropouts and a reduced number of artifacts compared to both an increase in injection time and increase in the number of amplification cycles.

Original language	English
Journal	Forensic Science International: Genetics Supplement Series
Volume	3
Issue number	1
Pages (from-to)	e347-e348
Number of pages	2
ISSN	1875-1768
DOIs	https://doi.org/10.1016/j.fsigss.2011.09.036
Publication status	Published - Dec 2011

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10.1016/j.fsigss.2011.09.036

Purification and concentration of PCR products leads to increased signal intensities with fewer allelic drop-outs and artifactsFinal published version, 325 KB

http://www.fsigeneticssup.com/article/S1875-1768%2811%2900174-0/fulltext

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29 Aug 2011 → 3 Sept 2011
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Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts. / Maria Irlund Pedersen, Line; Stangegaard, Michael; Mogensen, Helle Smidt et al.
In: Forensic Science International: Genetics Supplement Series, Vol. 3, No. 1, 12.2011, p. e347-e348.

Research output: Contribution to journal › Conference article › Research › peer-review

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abstract = "Capillary electrophoresis of amplified DNA isolated from trace evidence samples occasionally results in inadequate STR-profiles due to artifacts caused by e.g. primers and dNTPs. Removal of artifacts by purification and subsequent concentration of the PCR products may increase the sensitivity and the quality of the DNA profiles without re-amplification of the sample. We have validated and implemented an automated method to purify and 2-fold concentrate PCR products resulting in allelic peaks with higher intensity (a median height across all loci from 130 to 404 RFU), fewer allelic dropouts and a reduced number of artifacts compared to both an increase in injection time and increase in the number of amplification cycles.",

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AU - Morling, Niels

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DO - 10.1016/j.fsigss.2011.09.036

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ER -

Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts

Abstract

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