Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts

Line Maria Irlund Pedersen; Michael Stangegaard; Helle Smidt Mogensen; Niels Morling

doi:10.1016/j.fsigss.2011.09.036

Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts

Line Maria Irlund Pedersen, Michael Stangegaard, Helle Smidt Mogensen, Niels Morling

2 Citationer (Scopus)

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Abstract

Capillary electrophoresis of amplified DNA isolated from trace evidence samples occasionally results in inadequate STR-profiles due to artifacts caused by e.g. primers and dNTPs. Removal of artifacts by purification and subsequent concentration of the PCR products may increase the sensitivity and the quality of the DNA profiles without re-amplification of the sample. We have validated and implemented an automated method to purify and 2-fold concentrate PCR products resulting in allelic peaks with higher intensity (a median height across all loci from 130 to 404 RFU), fewer allelic dropouts and a reduced number of artifacts compared to both an increase in injection time and increase in the number of amplification cycles.

Originalsprog	Engelsk
Tidsskrift	Forensic Science International: Genetics Supplement Series
Vol/bind	3
Udgave nummer	1
Sider (fra-til)	e347-e348
Antal sider	2
ISSN	1875-1768
DOI	https://doi.org/10.1016/j.fsigss.2011.09.036
Status	Udgivet - dec. 2011

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10.1016/j.fsigss.2011.09.036

Purification and concentration of PCR products leads to increased signal intensities with fewer allelic drop-outs and artifactsForlagets udgivne version, 325 KB

http://www.fsigeneticssup.com/article/S1875-1768%2811%2900174-0/fulltext

ISFG
Stangegaard, M. (Deltager)
29 aug. 2011 → 3 sep. 2011
Aktivitet: Deltagelse i eller arrangement af en begivenhed - typer › Deltagelse i workshop, seminar og kursus

Citationsformater

Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts. / Maria Irlund Pedersen, Line; Stangegaard, Michael; Mogensen, Helle Smidt et al.
I: Forensic Science International: Genetics Supplement Series, Bind 3, Nr. 1, 12.2011, s. e347-e348.

Publikation: Bidrag til tidsskrift › Konferenceartikel › Forskning › peer review

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abstract = "Capillary electrophoresis of amplified DNA isolated from trace evidence samples occasionally results in inadequate STR-profiles due to artifacts caused by e.g. primers and dNTPs. Removal of artifacts by purification and subsequent concentration of the PCR products may increase the sensitivity and the quality of the DNA profiles without re-amplification of the sample. We have validated and implemented an automated method to purify and 2-fold concentrate PCR products resulting in allelic peaks with higher intensity (a median height across all loci from 130 to 404 RFU), fewer allelic dropouts and a reduced number of artifacts compared to both an increase in injection time and increase in the number of amplification cycles.",

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AU - Morling, Niels

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N2 - Capillary electrophoresis of amplified DNA isolated from trace evidence samples occasionally results in inadequate STR-profiles due to artifacts caused by e.g. primers and dNTPs. Removal of artifacts by purification and subsequent concentration of the PCR products may increase the sensitivity and the quality of the DNA profiles without re-amplification of the sample. We have validated and implemented an automated method to purify and 2-fold concentrate PCR products resulting in allelic peaks with higher intensity (a median height across all loci from 130 to 404 RFU), fewer allelic dropouts and a reduced number of artifacts compared to both an increase in injection time and increase in the number of amplification cycles.

AB - Capillary electrophoresis of amplified DNA isolated from trace evidence samples occasionally results in inadequate STR-profiles due to artifacts caused by e.g. primers and dNTPs. Removal of artifacts by purification and subsequent concentration of the PCR products may increase the sensitivity and the quality of the DNA profiles without re-amplification of the sample. We have validated and implemented an automated method to purify and 2-fold concentrate PCR products resulting in allelic peaks with higher intensity (a median height across all loci from 130 to 404 RFU), fewer allelic dropouts and a reduced number of artifacts compared to both an increase in injection time and increase in the number of amplification cycles.

U2 - 10.1016/j.fsigss.2011.09.036

DO - 10.1016/j.fsigss.2011.09.036

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JO - Forensic Science International: Genetics Supplement Series

JF - Forensic Science International: Genetics Supplement Series

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Purification and Concentration of PCR Products Leads to Increased Signal intensities with Fewer Allelic Drop-Outs and Artifacts

Abstract

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