Abstract
Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.
| Original language | English |
|---|---|
| Journal | FEBS Letters |
| Volume | 229 |
| Issue number | 1 |
| Pages (from-to) | 73-6 |
| Number of pages | 4 |
| ISSN | 0014-5793 |
| Publication status | Published - 29 Feb 1988 |
Keywords
- Animals
- Base Sequence
- Binding Sites
- Cattle
- DNA/metabolism
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases/metabolism
- Ficusin/metabolism
- Furocoumarins/metabolism
- Molecular Sequence Data
- Photochemistry
- Protein Binding
- Repressor Proteins/metabolism
- Trioxsalen/metabolism
- Viral Proteins
- Viral Regulatory and Accessory Proteins