Psoralen photofootprinting of protein-binding sites on DNA

W P Zhen; Peter E. Nielsen

Psoralen photofootprinting of protein-binding sites on DNA

Transcription, RNA, and Gene Medicine Program

15 Citationer (Scopus)

Abstract

Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.

Originalsprog	Engelsk
Tidsskrift	FEBS Letters
Vol/bind	229
Udgave nummer	1
Sider (fra-til)	73-6
Antal sider	4
ISSN	0014-5793
Status	Udgivet - 29 feb. 1988

Citationsformater

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abstract = "Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.",

keywords = "Animals, Base Sequence, Binding Sites, Cattle, DNA/metabolism, DNA-Binding Proteins, DNA-Directed RNA Polymerases/metabolism, Ficusin/metabolism, Furocoumarins/metabolism, Molecular Sequence Data, Photochemistry, Protein Binding, Repressor Proteins/metabolism, Trioxsalen/metabolism, Viral Proteins, Viral Regulatory and Accessory Proteins",

author = "Zhen, {W P} and Nielsen, {Peter E.}",

year = "1988",

month = feb,

day = "29",

language = "English",

volume = "229",

pages = "73--6",

journal = "F E B S Letters",

issn = "0014-5793",

publisher = "JohnWiley & Sons Ltd",

number = "1",

}

TY - JOUR

T1 - Psoralen photofootprinting of protein-binding sites on DNA

AU - Zhen, W P

AU - Nielsen, Peter E.

PY - 1988/2/29

Y1 - 1988/2/29

N2 - Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.

AB - Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.

KW - Animals

KW - Base Sequence

KW - Binding Sites

KW - Cattle

KW - DNA/metabolism

KW - DNA-Binding Proteins

KW - DNA-Directed RNA Polymerases/metabolism

KW - Ficusin/metabolism

KW - Furocoumarins/metabolism

KW - Molecular Sequence Data

KW - Photochemistry

KW - Protein Binding

KW - Repressor Proteins/metabolism

KW - Trioxsalen/metabolism

KW - Viral Proteins

KW - Viral Regulatory and Accessory Proteins

M3 - Journal article

C2 - 3345840

SN - 0014-5793

VL - 229

SP - 73

EP - 76

JO - F E B S Letters

JF - F E B S Letters

IS - 1

ER -

Psoralen photofootprinting of protein-binding sites on DNA

Abstract

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