Abstract
Glucagon-like peptide 2 (GLP-2) is a 33-amino acid (1-33) intestinotrophic peptide. In this study, the distribution and binding of i.v. injected radiolabeled GLP-2 (1-33) were investigated in rats using autoradiography in order to target possible binding sites. The major part of (125)I-GLP-2 (1-33) was distributed to kidneys, liver, and the gastrointestinal tract. In the small intestine, a high density of grains was localized in the epithelium with a predominance in the luminal part of the villus. The saturability of (125)I-GLP-2 (1-33) was investigated by administration of excess amounts of non-radioactive GLP-2 (1-33) or the primary metabolite of GLP-2 degradation, GLP-2 (3-33). In the small intestine, (125)I-GLP-2 was displaced both by non-radioactive GLP-2 (1-33) and (3-33), suggesting that the uptake of GLP-2 (1-33) in the small intestine is receptor-specific and that the metabolite GLP-2 (3-33) may interact with the GLP-2 receptor.
| Original language | English |
|---|---|
| Journal | Peptides |
| Volume | 21 |
| Issue number | 10 |
| Pages (from-to) | 1511-7 |
| Number of pages | 7 |
| ISSN | 0196-9781 |
| Publication status | Published - Oct 2000 |
Keywords
- Animals
- Autoradiography
- Binding, Competitive
- Epithelium
- Female
- Glucagon-Like Peptide 2
- Glucagon-Like Peptides
- Injections, Intravenous
- Intestine, Small
- Iodine Radioisotopes
- Kidney
- Liver
- Peptides
- Protein Binding
- Radioimmunoassay
- Rats
- Rats, Wistar
- Receptors, Glucagon
- Substrate Specificity