Abstract
It is demonstrated that DNA photofootprinting analysis of the intercalating depsipeptide echinomycin, and the minor groove-binders distamicyn, 4',6-diamidino-2-phenylindole (DAPI) and Hoechst 33258 can be performed using 9-[6-(2-diazocyclopentadienylcarbonyloxy)hexylamino]acridine (DHA) [Nielsen et al. (1988) Nucleic Acids Res. 16, 3877-3888] or 2-methoxy-6-azido-9-aminoacridine (MAA) [Jeppesen et al. (1988) Nucleic Acids Res. 16, 5755-5770]. Both the extent of the drug-binding sites and their relative strength can be determined with either reagent. DNA has the advantage of giving virtually sequence-uniform DNA photocleavage. On the other hand, structural changes in the DNA are detected by MAA. Using the 232-base-pair EcoRI-PvuII pUC19 restriction fragment, it is found that cleavage protection by distamycin, DAPI and Hoechst 33258 all require an (A.T)4 sequence, whereas protection by echinomycin was confined to a G + C-rich 8-base-pair region.
| Original language | English |
|---|---|
| Journal | European Journal of Biochemistry |
| Volume | 182 |
| Issue number | 2 |
| Pages (from-to) | 437-44 |
| Number of pages | 8 |
| ISSN | 0014-2956 |
| Publication status | Published - 15 Jun 1989 |
Keywords
- Aminoacridines
- Azides
- Base Sequence
- Bisbenzimidazole/analysis
- DNA/analysis
- DNA Damage
- DNA Restriction Enzymes
- Distamycins/analysis
- Echinomycin/analysis
- Indoles/analysis
- Molecular Sequence Data
- Molecular Structure
- Plasmids
- Receptors, Drug/analysis