Abstract
The synthesis and testing of a new type of nucleic acid-protein photocrosslinking reagent is described. The reagents are composed of a psoralen ligand for nucleic acid photoattachment, which is linked to an azidobenzoyl group, for protein photoattachment. The linker contains a disulfide bridge which can be opened by reduction with mercaptans. The reagents were tested in a chromatin system, where it was found that they induced cleavable crosslinks between the histones and the DNA upon irradiation with long-wavelength ultraviolet light (lambda greater than 300 nm).
| Original language | English |
|---|---|
| Journal | Analytical Biochemistry |
| Volume | 149 |
| Issue number | 2 |
| Pages (from-to) | 575-81 |
| Number of pages | 7 |
| ISSN | 0003-2697 |
| Publication status | Published - Sept 1985 |
Keywords
- Azides/chemical synthesis
- Chromatin/radiation effects
- Cross-Linking Reagents/chemical synthesis
- Cystamine/chemical synthesis
- DNA/radiation effects
- Furocoumarins/chemical synthesis
- Histones/radiation effects
- Nucleoproteins/radiation effects
- Photochemistry
- Ultraviolet Rays