TY - JOUR
T1 - Photochemical crosslinking of protein and DNA in chromatin. II. Synthesis and application of psoralen-cystamine-arylazido photocrosslinking reagents
AU - Elsner, H
AU - Buchardt, O
AU - Nielsen, Peter E.
PY - 1985/9
Y1 - 1985/9
N2 - The synthesis and testing of a new type of nucleic acid-protein photocrosslinking reagent is described. The reagents are composed of a psoralen ligand for nucleic acid photoattachment, which is linked to an azidobenzoyl group, for protein photoattachment. The linker contains a disulfide bridge which can be opened by reduction with mercaptans. The reagents were tested in a chromatin system, where it was found that they induced cleavable crosslinks between the histones and the DNA upon irradiation with long-wavelength ultraviolet light (lambda greater than 300 nm).
AB - The synthesis and testing of a new type of nucleic acid-protein photocrosslinking reagent is described. The reagents are composed of a psoralen ligand for nucleic acid photoattachment, which is linked to an azidobenzoyl group, for protein photoattachment. The linker contains a disulfide bridge which can be opened by reduction with mercaptans. The reagents were tested in a chromatin system, where it was found that they induced cleavable crosslinks between the histones and the DNA upon irradiation with long-wavelength ultraviolet light (lambda greater than 300 nm).
KW - Azides/chemical synthesis
KW - Chromatin/radiation effects
KW - Cross-Linking Reagents/chemical synthesis
KW - Cystamine/chemical synthesis
KW - DNA/radiation effects
KW - Furocoumarins/chemical synthesis
KW - Histones/radiation effects
KW - Nucleoproteins/radiation effects
KW - Photochemistry
KW - Ultraviolet Rays
M3 - Journal article
C2 - 4073511
SN - 0003-2697
VL - 149
SP - 575
EP - 581
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -