Perturbations of NAD+ salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle

Marianne Agerholm Andersen; Morten Dall; Benjamin Anderschou Holbech Jensen; Clara Prats; Søren Madsen; Astrid Linde Basse; Anne-Sofie Graae; Steve Risis; Julie Goldenbaum; Bjørn Quistorff; Steen Larsen; Sara Gry Vienberg; Jonas Thue Treebak

doi:10.1152/ajpendo.00213.2017

Perturbations of NAD⁺ salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle

Marianne Agerholm Andersen, Morten Dall, Benjamin Anderschou Holbech Jensen, Clara Prats, Søren Madsen, Astrid Linde Basse, Anne-Sofie Graae, Steve Risis, Julie Goldenbaum, Bjørn Quistorff, Steen Larsen, Sara Gry Vienberg, Jonas Thue Treebak

19 Citations (Scopus)

Abstract

Nicotinamide adenine dinucleotide (NAD⁺) can be synthesized by nicotinamide phosphoribosyltransferase (NAMPT). We aimed to determine the role of NAMPT in maintaining NAD⁺ levels, mitochondrial function, and metabolic homeostasis in skeletal muscle cells. We generated stable Nampt knockdown (shNampt KD) C2C12 cells using a shRNA lentiviral approach. Moreover, we applied gene electrotransfer to express Cre recombinase in tibialis anterior muscle of floxed Nampt mice. In shNampt KD C2C12 myoblasts, Nampt and NAD⁺ levels were reduced by 70% and 50%, respectively, and maximal respiratory capacity was reduced by 25%. Moreover, anaerobic glycolytic flux increased by 55%, and 2-deoxyglucose uptake increased by 25% in shNampt KD cells. Treatment with the NAD⁺ precursor nicotinamide riboside restored NAD⁺ levels in shNampt cells and increased maximal respiratory capacity by 18% and 32% in control and shNampt KD cells, respectively. Expression of Cre recombinase in muscle of floxed Nampt mice reduced NAMPT and NAD⁺ levels by 38% and 43%, respectively. Glucose uptake increased by 40%, and mitochondrial complex IV respiration was compromised by 20%. Hypoxiainducible factor (HIF)-1α-regulated genes and histone H3 lysine 9 (H3K9) acetylation, a known sirtuin 6 (SIRT6) target, were increased in shNampt KD cells. Thus, we propose that the shift toward glycolytic metabolism observed, at least in part, is mediated by the SIRT6/ HIF1α axis. Our findings suggest that NAMPT plays a key role for maintaining NAD⁺ levels in skeletal muscle and that NAMPT deficiency compromises oxidative phosphorylation capacity and alters energy homeostasis in this tissue.

Original language	English
Journal	American Journal of Physiology: Endocrinology and Metabolism
Volume	314
Issue number	4
Pages (from-to)	E377-E395
Number of pages	19
ISSN	0193-1849
DOIs	https://doi.org/10.1152/ajpendo.00213.2017
Publication status	Published - Apr 2018

Keywords

Journal Article

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10.1152/ajpendo.00213.2017

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Andersen, M. A., Dall, M., Jensen, B. A. H., Prats, C., Madsen, S., Basse, A. L., Graae, A-S., Risis, S., Goldenbaum, J., Quistorff, B., Larsen, S., Vienberg, S. G., & Treebak, J. T. (2018). Perturbations of NAD⁺ salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle. American Journal of Physiology: Endocrinology and Metabolism, 314(4), E377-E395. https://doi.org/10.1152/ajpendo.00213.2017

Perturbations of NAD⁺ salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle. / Andersen, Marianne Agerholm; Dall, Morten ; Jensen, Benjamin Anderschou Holbech et al.

In: American Journal of Physiology: Endocrinology and Metabolism, Vol. 314, No. 4, 04.2018, p. E377-E395.

Research output: Contribution to journal › Journal article › Research › peer-review

Andersen, MA, Dall, M , Jensen, BAH , Prats, C, Madsen, S, Basse, AL, Graae, A-S, Risis, S, Goldenbaum, J, Quistorff, B , Larsen, S, Vienberg, SG & Treebak, JT 2018, 'Perturbations of NAD⁺ salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle', American Journal of Physiology: Endocrinology and Metabolism, vol. 314, no. 4, pp. E377-E395. https://doi.org/10.1152/ajpendo.00213.2017

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abstract = "Nicotinamide adenine dinucleotide (NAD+) can be synthesized by nicotinamide phosphoribosyltransferase (NAMPT). We aimed to determine the role of NAMPT in maintaining NAD+ levels, mitochondrial function, and metabolic homeostasis in skeletal muscle cells. We generated stable Nampt knockdown (shNampt KD) C2C12 cells using a shRNA lentiviral approach. Moreover, we applied gene electrotransfer to express Cre recombinase in tibialis anterior muscle of floxed Nampt mice. In shNampt KD C2C12 myoblasts, Nampt and NAD+ levels were reduced by 70% and 50%, respectively, and maximal respiratory capacity was reduced by 25%. Moreover, anaerobic glycolytic flux increased by 55%, and 2-deoxyglucose uptake increased by 25% in shNampt KD cells. Treatment with the NAD+ precursor nicotinamide riboside restored NAD+ levels in shNampt cells and increased maximal respiratory capacity by 18% and 32% in control and shNampt KD cells, respectively. Expression of Cre recombinase in muscle of floxed Nampt mice reduced NAMPT and NAD+ levels by 38% and 43%, respectively. Glucose uptake increased by 40%, and mitochondrial complex IV respiration was compromised by 20%. Hypoxiainducible factor (HIF)-1α-regulated genes and histone H3 lysine 9 (H3K9) acetylation, a known sirtuin 6 (SIRT6) target, were increased in shNampt KD cells. Thus, we propose that the shift toward glycolytic metabolism observed, at least in part, is mediated by the SIRT6/ HIF1α axis. Our findings suggest that NAMPT plays a key role for maintaining NAD+ levels in skeletal muscle and that NAMPT deficiency compromises oxidative phosphorylation capacity and alters energy homeostasis in this tissue.",

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T1 - Perturbations of NAD+ salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle

AU - Andersen, Marianne Agerholm

AU - Dall, Morten

AU - Jensen, Benjamin Anderschou Holbech

AU - Prats, Clara

AU - Madsen, Søren

AU - Basse, Astrid Linde

AU - Graae, Anne-Sofie

AU - Risis, Steve

AU - Goldenbaum, Julie

AU - Quistorff, Bjørn

AU - Larsen, Steen

AU - Vienberg, Sara Gry

AU - Treebak, Jonas Thue

PY - 2018/4

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N2 - Nicotinamide adenine dinucleotide (NAD+) can be synthesized by nicotinamide phosphoribosyltransferase (NAMPT). We aimed to determine the role of NAMPT in maintaining NAD+ levels, mitochondrial function, and metabolic homeostasis in skeletal muscle cells. We generated stable Nampt knockdown (shNampt KD) C2C12 cells using a shRNA lentiviral approach. Moreover, we applied gene electrotransfer to express Cre recombinase in tibialis anterior muscle of floxed Nampt mice. In shNampt KD C2C12 myoblasts, Nampt and NAD+ levels were reduced by 70% and 50%, respectively, and maximal respiratory capacity was reduced by 25%. Moreover, anaerobic glycolytic flux increased by 55%, and 2-deoxyglucose uptake increased by 25% in shNampt KD cells. Treatment with the NAD+ precursor nicotinamide riboside restored NAD+ levels in shNampt cells and increased maximal respiratory capacity by 18% and 32% in control and shNampt KD cells, respectively. Expression of Cre recombinase in muscle of floxed Nampt mice reduced NAMPT and NAD+ levels by 38% and 43%, respectively. Glucose uptake increased by 40%, and mitochondrial complex IV respiration was compromised by 20%. Hypoxiainducible factor (HIF)-1α-regulated genes and histone H3 lysine 9 (H3K9) acetylation, a known sirtuin 6 (SIRT6) target, were increased in shNampt KD cells. Thus, we propose that the shift toward glycolytic metabolism observed, at least in part, is mediated by the SIRT6/ HIF1α axis. Our findings suggest that NAMPT plays a key role for maintaining NAD+ levels in skeletal muscle and that NAMPT deficiency compromises oxidative phosphorylation capacity and alters energy homeostasis in this tissue.

AB - Nicotinamide adenine dinucleotide (NAD+) can be synthesized by nicotinamide phosphoribosyltransferase (NAMPT). We aimed to determine the role of NAMPT in maintaining NAD+ levels, mitochondrial function, and metabolic homeostasis in skeletal muscle cells. We generated stable Nampt knockdown (shNampt KD) C2C12 cells using a shRNA lentiviral approach. Moreover, we applied gene electrotransfer to express Cre recombinase in tibialis anterior muscle of floxed Nampt mice. In shNampt KD C2C12 myoblasts, Nampt and NAD+ levels were reduced by 70% and 50%, respectively, and maximal respiratory capacity was reduced by 25%. Moreover, anaerobic glycolytic flux increased by 55%, and 2-deoxyglucose uptake increased by 25% in shNampt KD cells. Treatment with the NAD+ precursor nicotinamide riboside restored NAD+ levels in shNampt cells and increased maximal respiratory capacity by 18% and 32% in control and shNampt KD cells, respectively. Expression of Cre recombinase in muscle of floxed Nampt mice reduced NAMPT and NAD+ levels by 38% and 43%, respectively. Glucose uptake increased by 40%, and mitochondrial complex IV respiration was compromised by 20%. Hypoxiainducible factor (HIF)-1α-regulated genes and histone H3 lysine 9 (H3K9) acetylation, a known sirtuin 6 (SIRT6) target, were increased in shNampt KD cells. Thus, we propose that the shift toward glycolytic metabolism observed, at least in part, is mediated by the SIRT6/ HIF1α axis. Our findings suggest that NAMPT plays a key role for maintaining NAD+ levels in skeletal muscle and that NAMPT deficiency compromises oxidative phosphorylation capacity and alters energy homeostasis in this tissue.

KW - Journal Article

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DO - 10.1152/ajpendo.00213.2017

M3 - Journal article

C2 - 29208611

SN - 0193-1849

VL - 314

SP - E377-E395

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

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